2023
DOI: 10.1021/acssynbio.3c00070
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A Hitchhiker’s Guide to Supplying Enzymatic Reducing Power into Synthetic Cells

Abstract: The construction from scratch of synthetic cells by assembling molecular building blocks is unquestionably an ambitious goal from a scientific and technological point of view. To realize functional life-like systems, minimal enzymatic modules are required to sustain the processes underlying the out-ofequilibrium thermodynamic status hallmarking life, including the essential supply of energy in the form of electrons. The nicotinamide cofactors NAD(H) and NADP(H) are the main electron carriers fueling reductive … Show more

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Cited by 5 publications
(3 citation statements)
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“…Particularly, Fdh SNO has recently been successfully used as a pivotal component of a minimal enzymatic pathway triggered by formate ensuring the redox regeneration in the field of bottom-up synthetic biology [14,63]. Furthermore, the manufacture of valuable enantiomers for different industries [25] could represent an application for this biocatalyst, both in in vivo and in vitro systems.…”
Section: Discussionmentioning
confidence: 99%
“…Particularly, Fdh SNO has recently been successfully used as a pivotal component of a minimal enzymatic pathway triggered by formate ensuring the redox regeneration in the field of bottom-up synthetic biology [14,63]. Furthermore, the manufacture of valuable enantiomers for different industries [25] could represent an application for this biocatalyst, both in in vivo and in vitro systems.…”
Section: Discussionmentioning
confidence: 99%
“…SCs then become competent for enzyme reactions, gene expression (as specified above), metabolic pathways for lipid synthesis [ 136 ], simple forms of control based on the activation of gene expression, the enzymatic production of signal molecules, sensing signal molecules, the formation of pores and gap junctions [ 137 , 138 ] in the membrane and periodic reaction–diffusion waves [ 139 ], genetic material replication, and the production of toxins [ 140 , 141 , 142 ]. Additional relevant functions, like bio-energy generation, have recently been achieved [ 105 , 143 , 144 , 145 ], while others are under preliminary analyses (e.g., reducing power for metabolism [ 146 ]; chemical neural networks for computation and control [ 114 ]). These functions have generally been implemented individually, one at a time.…”
Section: Bottom-up Scs: Principles Construction and Featuresmentioning
confidence: 99%
“…SCs with smaller internal compartments are used as models of eukaryotic cells, or just to achieve specific and more complex behaviors [ 131 ]. Interested readers should refer to recent reviews, e.g., [ 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 ].…”
Section: Appendix A1 Pioneering Work On Bottom-up Synthetic Cellsmentioning
confidence: 99%