A Homeostatic Sleep-Stabilizing Pathway in Drosophila Composed of the Sex Peptide Receptor and Its Ligand, the Myoinhibitory Peptide

Oh, Yangkyun; Yoon, Sung Eun; Zhang, Qi; Chae, Hyo Seok; Daubnerová, Ivana; Shafer, Orie T.; Choe, Joonho; Kim, Young-Joon

doi:10.1371/journal.pbio.1001974

Cited by 73 publications

(65 citation statements)

References 73 publications

(143 reference statements)

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“…We measured locomotor activity as previously described [41]. We collected adult males at eclosion and aged them in groups of 10–15 for 3 d. We loaded individual 3-d-old flies in 65 x 5 mm glass tubes containing 4% sucrose and 2% agar and measured their locomotor activity in 1 min bins with a DAM System monitor (Trikinetics) inside an incubator at 25°C and 50% humidity.…”

Section: Methodsmentioning

confidence: 99%

A fat-derived metabolite regulates a peptidergic feeding circuit in Drosophila

et al. 2017

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Here, we show that the enzymatic cofactor tetrahydrobiopterin (BH4) inhibits feeding in Drosophila. BH4 biosynthesis requires the sequential action of the conserved enzymes Punch, Purple, and Sepiapterin Reductase (Sptr). Although we observe increased feeding upon loss of Punch and Purple in the adult fat body, loss of Sptr must occur in the brain. We found Sptr expression is required in four adult neurons that express neuropeptide F (NPF), the fly homologue of the vertebrate appetite regulator neuropeptide Y (NPY). As expected, feeding flies BH4 rescues the loss of Punch and Purple in the fat body and the loss of Sptr in NPF neurons. Mechanistically, we found BH4 deficiency reduces NPF staining, likely by promoting its release, while excess BH4 increases NPF accumulation without altering its expression. We thus show that, because of its physically distributed biosynthesis, BH4 acts as a fat-derived signal that induces satiety by inhibiting the activity of the NPF neurons.

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Section: Methodsmentioning

confidence: 99%

A fat-derived metabolite regulates a peptidergic feeding circuit in Drosophila

et al. 2017

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“…Specifically, SPR is also expressed in the brains of larvae and adult males, and has similar affinity for both SP and myoinhibitory peptides (MIPs) [145,146]. A recent study showed that MIP-SPR interactions play a critical role in Drosophila sleep behavior [147]. Third, there is little evidence for co-evolution between the two genes.…”

Section: Seminal Fluid Proteinsmentioning

confidence: 99%

From molecules to mating: Rapid evolution and biochemical studies of reproductive proteins

Wilburn

Swanson

2016

Journal of Proteomics

109

132

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Sexual reproduction and the exchange of genetic information are essential biological processes for species across all branches of the tree of life. Over the last four decades, biochemists have continued to identify many of the factors that facilitate reproduction, but the molecular mechanisms that mediate this process continue to elude us. However, a recurring observation in this research has been the rapid evolution of reproductive proteins. In animals, the competing interests of males and females often result in arms race dynamics between pairs of interacting proteins. This phenomenon has been observed in all stages of reproduction, including pheromones, seminal fluid components, and gamete recognition proteins. In this article, we review how the integration of evolutionary theory with biochemical experiments can be used to study interacting reproductive proteins. Examples are included from both model and non-model organisms, and recent studies are highlighted for their use of state-of-the-art genomic and proteomic techniques. Significance Despite decades of research, our understanding of the molecular mechanisms that mediate fertilization remain poorly characterized. To date, molecular evolutionary studies on both model and non-model organisms have provided some of the best inferences to elucidating the molecular underpinnings of animal reproduction. This review article details how biochemical and evolutionary experiments have jointly enhanced the field for 40 years, and how recent work using high-throughput genomic and proteomic techniques have shed additional insights into this crucial biological process.

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“…Recently, MIPs have been shown to be required for stabilizing sleep in both males and females. 12 Mutant flies deficient for MIPs sleep less than control because of lacking sleep maintenance abilities. The SPR-deficient flies also exhibited sleep defects as observed in MIPs mutant flies, suggesting that MIPs regulate sleep stabilization via the SPR activation.…”

Section: Sp/spr-mediated Post Mating Responsesmentioning

confidence: 99%

Evolution of sex-peptide in Drosophila

Tsuda

Aigaki

2016

Fly

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The Drosophila sex-peptide (SP) has been identified as a seminal fluid component that induces postmating responses (PMRs) in the inseminated females, such as inhibition of remating and stimulation of egg-laying. SP has been thought to play a central role in sexual conflict and sexually antagonistic co-evolution. Most of the sequenced Drosophila genomes contain SP orthologs, but their functions have been poorly characterized. Recently, we have investigated cross-species activity of D. melanogaster SP by means of injection into virgin females of other species. Among 11 species examined, SP response was observed in 6 species belonging to the D. melanogaster species group only. These species females express SP receptor (SPR) in their oviducts at relatively high levels, which was visualized by using a GFP-tagged SP. Furthermore, females of this species group responded to their own SP orthologs. However, females of the species outside the group did not respond to their own SP orthologs, even though all of them were potent inducers of SP-response in D. melanogaster. Our results suggested that the SP/SPR-mediated PMR was established in the lineage of the D. melanogaster species group.

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A Homeostatic Sleep-Stabilizing Pathway in Drosophila Composed of the Sex Peptide Receptor and Its Ligand, the Myoinhibitory Peptide

Abstract: A ligand of the sex peptide receptor maintains sleep stability and homeostasis by inhibiting wakefulness-promoting neurons in Drosophila.

Cited by 73 publications

References 73 publications

A fat-derived metabolite regulates a peptidergic feeding circuit in Drosophila

A fat-derived metabolite regulates a peptidergic feeding circuit in Drosophila

From molecules to mating: Rapid evolution and biochemical studies of reproductive proteins

Evolution of sex-peptide in Drosophila

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