A homogeneous electrochemical aptasensor based on <scp>DNA</scp> assembly for zearalenone detection

Qu, Chao; Xin, Lingkun; Yu, Songcheng; Wei, Min

doi:10.1002/jccs.202100136

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…The Chinese national standards specifics a maximum limit of less than 20 μg/kg in food in corn, corn flakes, and corn products. The common laboratory methods for detecting AFB1 are immunochromatography (Liu et al, 2020), chemiluminescence (Shim et al, 2014), high‐performance liquid chromatography (Zhang et al, 2020), chromatography–mass spectrometry (Dong et al, 2018), and so on (Qu et al, 2021). However, these approaches are time‐consuming and expensive and require sample preparation and trained personnel to operate the instruments.…”

Section: Introductionmentioning

confidence: 99%

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Detection of AFB1 in corn by MXene paper‐based unlabeled aptasensor

Wang,

Gu,

Rong

et al. 2024

J Food Process Engineering

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In this work, a paper‐based electrochemical aptamer sensor was developed for the detection of aflatoxin B1 (AFB1) using a combination of MXene–Ti3C2Tx and nucleic acid aptamers. The prepared single‐layer or few‐layer MXene suspension is suction‐filtered onto MXene paper, which is cut to prepare MXene electrodes. To accomplish AFB1 specific detection, an amino‐labeled AFB1 aptamer is mounted on the surface of the carboxy‐functionalized MXene electrode. When AFB1 is present, it particularly binds to the aptamer to form a 3D structure, reducing the efficiency of electron transmission on the sensor surface. The difference in impedance signal change at the electrode/electrolyte interface is used to quantify AFB1. The results indicated that the detection range is 0.05–100 ng/mL, the detection limit is 0.04 ng/mL, and the recovery rate of AFB1 in corn samples is 97.8%–111.52% with the optimal detection conditions. The MXene paper‐based label‐free aptasensor is versatile and can detect different targets by simply swapping out the aptamers of different targets. The sensor also has a wide range of applications in food analysis and environmental testing.Practical applicationsA paper‐based electrochemical aptamer sensor was developed to detect aflatoxin B1 using a combination of MXene–Ti3C2Tx and nucleic acid aptamers.The design is based on the preparation of MXene electrodes by pumping and filtering monolayer or multilayer MXene suspensions onto MXene paper and cutting.The MXene paper‐based label‐free aptamer sensor was designed to be versatile, allowing the detection of different targets by simply replacing the aptamer with one from a different targets.

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Section: Introductionmentioning

confidence: 99%

“…, chromatography-mass spectrometry (Dong et al, 2018), and so on (Qu et al, 2021). However, these approaches are timeconsuming and expensive and require sample preparation and trained personnel to operate the instruments.…”

mentioning

confidence: 99%

Detection of AFB1 in corn by MXene paper‐based unlabeled aptasensor

Wang,

Gu,

Rong

et al. 2024

J Food Process Engineering

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“…Since the aptamer for ZEN was screened in 2012, 8 it has been applied to the development of various aptasensors for ZEN. [9][10][11][12][13][14][15][16] In order to achieve efficient signal amplication, nucleic acid amplication strategies are powerful tools. 17 PCR is a classic nucleic acid amplication process, which requires complex thermal cycling.…”

Section: Introductionmentioning

confidence: 99%

An immobilization-free electrochemical aptamer-based assay for zearalenone based on target-triggered dissociation of DNA from polydopamine nanospheres with strand displacement amplification

et al. 2023

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“…Refs. [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 ] are cite in Supplementary Materials file.…”

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Evanescent Wave Optical-Fiber Aptasensor for Rapid Detection of Zearalenone in Corn with Unprecedented Sensitivity

et al. 2022

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Zearalenone (ZEN) is a common mycotoxin pollutant found in agricultural products. Aptamers are attractive recognition biomolecules for the development of mycotoxin biosensors. Even though numerous aptasensors have been reported for the detection of ZEN in recent years, many of them suffer from problems including low sensitivity, low specificity, tedious experimental steps, high-cost, and difficulty of automation. We report here the first evanescent wave optical-fiber aptasensor for the detection of ZEN with unprecedented sensitivity, high specificity, low cost, and easy of automation. In our aptasensor, a 40-nt ZEN-specific aptamer (8Z31) is covalently immobilized on the fiber. The 17-nt fluorophore Cy5.5-labeled complementary DNA strand and ZEN competitively bind with the aptamer immobilized on the fiber, enabling the signal-off fluorescent detection of ZEN. The coating of Tween 80 enhanced both the sensitivity and the reproducibility of the aptasensor. The sensor was able to detect ZEN spiked-in the corn flour extract with a semilog linear detection range of 10 pM-10 nM and a limit of detection (LOD, S/N = 3) of 18.4 ± 4.0 pM (equivalent to 29.3 ± 6.4 ng/kg). The LOD is more than 1000-fold lower than the maximum ZEN residue limits set by China (60 μg/kg) and EU (20 μg/kg). The sensor also has extremely high specificity and showed negligible cross-reactivity to other common mycotoxins. In addition, the sensor was able to be regenerated for 28 times, further decreasing its cost. Our sensor holds great potential for practical applications according to its multiple compelling features.

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A homogeneous electrochemical aptasensor based on DNA assembly for zearalenone detection

Cited by 5 publications

References 31 publications

Detection of AFB1 in corn by MXene paper‐based unlabeled aptasensor

Detection of AFB1 in corn by MXene paper‐based unlabeled aptasensor

An immobilization-free electrochemical aptamer-based assay for zearalenone based on target-triggered dissociation of DNA from polydopamine nanospheres with strand displacement amplification

Evanescent Wave Optical-Fiber Aptasensor for Rapid Detection of Zearalenone in Corn with Unprecedented Sensitivity

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