A Homolog of Albino3/OxaI Is Essential for Thylakoid Biogenesis in the Cyanobacterium Synechocystis sp. PCC6803

Spence, Edward; Bailey, Shaun; Nenninger, Anja; Møller, Simon Geir; Robinson, Colin

doi:10.1074/jbc.m411041200

Cited by 49 publications

(34 citation statements)

References 34 publications

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“…Only a single Alb3 gene has been found in all cyanobacterial genomes sequenced to date (Srl1471 in Synechocystis). Analysis of Srl1471 deletion mutants has shown that its product plays important roles in both cell division and membrane biogenesis (Spence et al, 2004). These findings indicate that Alb3 is essential for thylakoid protein biogenesis and that it may have a broad spectrum of substrates.…”

Section: R E T R a C T E Dmentioning

confidence: 86%

Retracted: LPA2 Is Required for Efficient Assembly of Photosystem II in Arabidopsis thaliana

Peng

Guo

et al. 2007

Plant Cell

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To elucidate the molecular mechanism of photosystem II (PSII) assembly, we characterized the low psii accumulation2 (lpa2) mutant of Arabidopsis thaliana, which is defective in the accumulation of PSII supercomplexes. The levels and processing patterns of the RNAs encoding the PSII subunits are unaltered in the mutant. In vivo protein-labeling experiments showed that the synthesis of CP43 (for chlorophyll a binding protein) was greatly reduced, but CP47, D1, and D2 were synthesized at normal rates in the lpa2-1 mutant. The newly synthesized CP43 was rapidly degraded in lpa2-1, and the turnover rates of D1 and D2 were higher in lpa2-1 than in wild-type plants. The newly synthesized PSII proteins were assembled into PSII complexes, but the assembly of PSII was less efficient in the mutant than in wild-type plants. LPA2 encodes an intrinsic thylakoid membrane protein, which is not an integral subunit of PSII. Yeast two-hybrid assays indicated that LPA2 interacts with the PSII core protein CP43 but not with the PSII reaction center proteins D1 and D2. Moreover, direct interactions of LPA2 with Albino3 (Alb3), which is involved in thylakoid membrane biogenesis and cell division, were also detected. Thus, the results suggest that LPA2, which appears to form a complex with Alb3, is involved in assisting CP43 assembly within PSII.

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Section: R E T R a C T E Dmentioning

confidence: 86%

Retracted: LPA2 Is Required for Efficient Assembly of Photosystem II in Arabidopsis thaliana

Peng

Guo

et al. 2007

Plant Cell

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“…PCC6803 contains just a single Alb3 gene (slr1471). It was shown that this Alb3 homologue is essential for cell viability and that depletion of this protein results in severe damage to thylakoid biogenesis (39). These data indicate that Alb3 plays a general role in protein insertion in Synechocystis as in other bacteria.…”

Section: Discussionmentioning

confidence: 61%

A Second Thylakoid Membrane-localized Alb3/OxaI/YidC Homologue Is Involved in Proper Chloroplast Biogenesis in Arabidopsis thaliana

Gerdes

Bals

Klostermann

et al. 2006

Journal of Biological Chemistry

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The integral membrane proteins Alb3, OxaI, and YidC belong to an evolutionary conserved protein family mediating protein insertion into the thylakoid membrane of chloroplasts, the inner membrane of mitochondria, and bacteria, respectively. Whereas OxaI and YidC are involved in the insertion of a wide range of membrane proteins, the function of Alb3 seems to be limited to the insertion of a subset of the light-harvesting chlorophyllbinding proteins. In this study, we identified a second chloroplast homologue of the Alb3/OxaI/YidC family, named Alb4. Alb4 is almost identical to the Alb3/OxaI/YidC domain of the previously described 110-kDa inner envelope protein Artemis. We show that Alb4 is expressed as a separate 55-kDa protein and that Artemis was identified mistakenly. Alb4 is located in the thylakoid membrane of Arabidopsis thaliana chloroplasts. Analysis of an Arabidopsis mutant (Salk_136199) and RNA interference lines with a reduced level of Alb4 revealed chloroplasts with an altered ultrastructure. Mutant plastids are larger and more spherical in appearance, and the grana stacks within the mutant lines are less appressed than in the wild-type chloroplasts. These data indicate that Alb4 is required for proper chloroplast biogenesis.

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“…This idea is supported by the fact that it is by now not possible to fully segregate a deletion mutant of slr1471. Previous results with partially segregated knockdown mutants suggested that Slr1471p plays a role not only in cell division but in membrane biogenesis as well (Spence et al, 2004). Here, we show that Slr1471p function is significantly impaired by the fusion of green fluorescent protein (GFP) to its C terminus.…”

Section: Introductionmentioning

confidence: 57%

The Synechocystis sp PCC 6803 Oxa1 Homolog Is Essential for Membrane Integration of Reaction Center Precursor Protein pD1

et al. 2006

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Synechocystis sp PCC 6803 Slr1471p, an Oxa1p/Alb3/YidC homolog, is an essential protein for cell viability for which functions in thylakoid membrane biogenesis and cell division have been proposed. Using a fusion of green fluorescent protein to the C terminus of Slr1471p, we found that the mutant slr1471-gfp is photochemically inhibited when light intensities increase to 80 μmol·m−2·s−1. We show that photoinhibition correlates with an increased redox potential of the reaction center quinone QA − and a decreased redox potential of QB −. Analysis reveals that membrane integration of the D1 precursor protein is affected, leading to the accumulation of pD1 in the membrane phase. We show that Slr1471p interacts directly with the D1 protein and discuss why the accumulation of pD1 in two reaction center assembly intermediates is dependent on Slr1471p.

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A Homolog of Albino3/OxaI Is Essential for Thylakoid Biogenesis in the Cyanobacterium Synechocystis sp. PCC6803

Cited by 49 publications

References 34 publications

Retracted: LPA2 Is Required for Efficient Assembly of Photosystem II in Arabidopsis thaliana

Retracted: LPA2 Is Required for Efficient Assembly of Photosystem II in Arabidopsis thaliana

A Second Thylakoid Membrane-localized Alb3/OxaI/YidC Homologue Is Involved in Proper Chloroplast Biogenesis in Arabidopsis thaliana

The Synechocystis sp PCC 6803 Oxa1 Homolog Is Essential for Membrane Integration of Reaction Center Precursor Protein pD1

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