A human monoclonal autoantibody isolated from a patient with infectious mononucleosis reactive with both self antigens and Epstein-Barr virus nuclear antigen (EBNA)

Garzelli, Carlo; Pacciardi, Anna; Carmignani, M.; Conaldi, Pier Giulio; Basolo, Fulvio; Falcone, Giuseppe

doi:10.1016/0165-2478(89)90193-4

Cited by 6 publications

(2 citation statements)

References 10 publications

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“…Anti-p542 reacts directly with EBNA-1, and this reactivity is inhibited by the gly/ala peptide P62. In similar findings, Garzelli et al (27) (29), each showed cross reactivity of immunoaffinity purified anti-gly/ala antibodies with keratin. The mimicry between p542 and EBNA-1 is, however, complicated.…”

Section: Discussionsupporting

confidence: 56%

Epstein-Barr virus-induced autoimmune responses. I. Immunoglobulin M autoantibodies to proteins mimicking and not mimicking Epstein-Barr virus nuclear antigen-1.

Vaughan

Valbracht²,

Nguyen³

et al. 1995

J. Clin. Invest.

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In previous studies of infectious mononucleosis, we found IgM autoantibodies which react with hematopoietic cell antigens. Many of these were inhibited by synthetic glycine/ alanine peptides representing the glycine/alanine repeat of Epstein-Barr virus nuclear antigen-i. We have cloned and expressed fragments of genes encoding two of these autoantigens. One gene (p542) encodes a protein containing a glycine-rich 28-mer, which is its chief autoantigenic epitope and which represents a newly identified class of evolutionarily conserved autoepitopes. The other gene (p554) encodes a protein that is not demonstrably cross-reactive with EpsteinBarr virus nuclear antigen-1 or with any other EBV protein, but forms complexes with other proteins. Immunoaffinitypurified anti-p542 and anti-p554 have relatively high binding affinities, as evidenced by inhibition at 106-108 M-', and neither autoantibody showed polyreactivity with other common antigens. The data thus suggest that neither autoantibody is simply an expression of polyclonal B cell activation. We conclude that the two autoantigens stimulate autoantibody synthesis by different mechanisms. One autoantigen shares homology to a viral protein which generates cross-reacting antibodies to the autoantigenic epitope. The other has no recognizable cross-reaction with the infecting pathogen and may become immunogenic through complexing with other proteins. (J. Clin. Invest. 1995Invest. . 95:1306Invest. -1315

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Section: Discussionsupporting

confidence: 56%

Epstein-Barr virus-induced autoimmune responses. I. Immunoglobulin M autoantibodies to proteins mimicking and not mimicking Epstein-Barr virus nuclear antigen-1.

Vaughan

Valbracht²,

Nguyen³

et al. 1995

J. Clin. Invest.

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“…EBV infections have been implicated in several autoimmune diseases such as multiple sclerosis, systemic lupus erythematosus, rheumatoid arthritis, and others (8). Autoimmune reactions are assumed to be triggered by antigenic mimicry (9), and antiviral drugs have been shown to cause remission of autoimmune disorders in some patients (10).…”

Section: Pstein-barr Virus (Ebv) Also Called Human Herpesvirus 4 (Hhv4)mentioning

confidence: 99%

CD4⁺and CD8⁺T-Cell Responses to Latent Antigen EBNA-1 and Lytic Antigen BZLF-1 during Persistent Lymphocryptovirus Infection of Rhesus Macaques

Leskowitz

Zhou

Villinger

et al. 2013

J Virol

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Epstein-Barr virus (EBV) infection leads to lifelong viral persistence through its latency in B cells. EBV-specific T cells controlreactivations and prevent the development of EBV-associated malignancies in most healthy carriers, but infection can sometimes cause chronic disease and malignant transformation. Epstein-Barr nuclear antigen 1 (EBNA-1) is the only viral protein consistently expressed during all forms of latency and in all EBV-associated malignancies and is a promising target for a therapeutic vaccine. Here, we studied the EBNA-1-specific immune response using the EBV-homologous rhesus lymphocryptovirus (rhLCV) infection in rhesus macaques. We assessed the frequency, phenotype, and cytokine production profiles of rhLCV EBNA-1 (rhEBNA-1)-specific T cells in 15 rhesus macaques and compared them to the lytic antigen of rhLCV BZLF-1 (rhBZLF-1). We were able to detect rhEBNA-1-specific CD4؉ and/or CD8 ؉ T cells in 14 of the 15 animals screened. In comparison, all 15 animals had detectable rhBZLF-1 responses. Most peptide-specific CD4؉ T cells exhibited a resting phenotype of central memory (TCM), while peptide-specific CD8؉ T cells showed a more activated phenotype, belonging mainly to the effector cell subset. By comparing our results to the human EBV immune response, we demonstrate that the rhLCV model is a valid system for studying chronic EBV infection and for the preclinical development of therapeutic vaccines.

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Epstein-Barr Virus and Autoimmunity

Garzelli

1996

Infectious Agents and Pathogenesis

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A human monoclonal autoantibody isolated from a patient with infectious mononucleosis reactive with both self antigens and Epstein-Barr virus nuclear antigen (EBNA)

Cited by 6 publications

References 10 publications

Epstein-Barr virus-induced autoimmune responses. I. Immunoglobulin M autoantibodies to proteins mimicking and not mimicking Epstein-Barr virus nuclear antigen-1.

Epstein-Barr virus-induced autoimmune responses. I. Immunoglobulin M autoantibodies to proteins mimicking and not mimicking Epstein-Barr virus nuclear antigen-1.

CD4⁺and CD8⁺T-Cell Responses to Latent Antigen EBNA-1 and Lytic Antigen BZLF-1 during Persistent Lymphocryptovirus Infection of Rhesus Macaques

Epstein-Barr Virus and Autoimmunity

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A human monoclonal autoantibody isolated from a patient with infectious mononucleosis reactive with both self antigens and Epstein-Barr virus nuclear antigen (EBNA)

Cited by 6 publications

References 10 publications

Epstein-Barr virus-induced autoimmune responses. I. Immunoglobulin M autoantibodies to proteins mimicking and not mimicking Epstein-Barr virus nuclear antigen-1.

Epstein-Barr virus-induced autoimmune responses. I. Immunoglobulin M autoantibodies to proteins mimicking and not mimicking Epstein-Barr virus nuclear antigen-1.

CD4+and CD8+T-Cell Responses to Latent Antigen EBNA-1 and Lytic Antigen BZLF-1 during Persistent Lymphocryptovirus Infection of Rhesus Macaques

Epstein-Barr Virus and Autoimmunity

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Product

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CD4⁺and CD8⁺T-Cell Responses to Latent Antigen EBNA-1 and Lytic Antigen BZLF-1 during Persistent Lymphocryptovirus Infection of Rhesus Macaques