A Human Novel Gene DERPC Located on 16q22.1 Inhibits Prostate Tumor Cell Growth and Its Expression Is Decreased in Prostate and Renal Tumors

Abstract

Background: Deletion of chromosome 16q is frequently associated with diverse tumors. Numerous studies strongly suggest the presence of one or more tumor suppressor genes on chromosome 16q22 to 16qter including the widely studied cadherin gene family. However, the specific tumor suppressor genes residing in this region need better definition and characterization. Material and Methods: Standard molecular biology approaches have been used to clone and characterize the DERPC cDNA and its protein product on chromosome 16q22.1. Northern blotting was used to define the expression pattern in a multiple human tissue blots. DERPC expression was examined in multi-tumor array (Clontech, CA, USA) dot blot as well as in laser capture microdissection (LCM) derived prostate cancer (CaP) specimens by quantitative RT-PCR. Western blot analysis and a fluorescent microscopy were used to characterize the molecular size and the cellular location of green

Integration Site Preference of Xenotropic Murine Leukemia Virus-Related Virus, a New Human Retrovirus Associated with Prostate Cancer

“…This region contains 115 RefSeq genes, many of these associated with cancer. Among these genes, the CDH1 (Ecadherin), DERPC (decreased expression in renal and prostate cancer), NFATc3 (nuclear factor of activated T-cell, cytoplasmic, calcineurin-dependent 3), and HAS3 (hyaluronan synthase 3) genes have been directly linked to prostate cancer development (10,57,93,96). Therefore, genetic instability in this region may have an important contribution to prostate cancer.…”

Loss of expression of chromosome 16q genes DPEP1 and CTCF in lobular carcinoma in situ of the breast

“…If DPEP1 expression is reduced less glutathione is produced, increasing oxidative stress, which may increase carcinogenesis [31]. Although loss of expression of the novel gene DERPC has been found in renal and prostate tumours [21], no link has been found with breast cancer. The results using RT-PCR show no association between DERPC and LCIS.…”

“…It is ubiquitously expressed with abundant expression in kidney, skeletal muscle, testis, liver, ovary and heart, with moderate expression in prostate [21]. Expression of DERPC has inhibitory potential on prostate cancer cell growth.…”

“…Expression of DERPC has inhibitory potential on prostate cancer cell growth. It has been found to be significantly decreased in renal tumours and prostate tumours [21]. Its role in breast cancer is unknown.…”

Methylthioadenosine (MTA) Regulates Liver Cells Proteome and Methylproteome: Implications in Liver Biology and Disease

“…For instance, hnRNP-K inhibition increases apoptosis in response to cytotoxic agents (80), while hnRNP-AB promotes proliferation and metastasis of pancreatic cancer cells (81). In addition to RBPs, other proteins whose methylation has been demonstrated in this study for the first time (CTF8A and TFG) might also explain the enhanced survival capacity of liver tumor cells (82,83) and how it might be regulated by MTA.…”