A human salivary protein which promotes adhesion of Streptococcus mutans serotype c strains to hydroxyapatite

Kishimoto, Etsuo; Hay, D.I.; Gibbons, R. J.

doi:10.1128/iai.57.12.3702-3707.1989

Cited by 57 publications

(20 citation statements)

References 47 publications

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“…Nonspecific hydrophobic (7, 14, 24, 40, 44) and ionic (7,9,24,44) interactions are involved. In additioti, adhesion to S-HA of Streptococcus mutatis JBP has been found to be tnediated by high-molecular-weight components from the salivary mucin fraction (15,22), whereas the adhesion of S. .sobrinus 6715 was augtnetited by the presence of glucans in the pellicles (13). For S. sanguis.…”

Section: Discussionmentioning

confidence: 96%

In vitro modulation of oral bacterial adhesion to saliva‐coated hydroxyapatite beads by milk casein derivatives

Neeser

Golliard

Woltz

et al. 1994

Oral Microbiology and Immunology

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Bovine caseinate, derivatives of its glycosylated moiety [caseinoglycomacropeptide (CGP)], and caseinophosphopeptides were evaluated as inhibitors of adhesion of oral bacteria to saliva-coated hydroxyapatite beads (S-HA). All milk casein-derived components behaved as potent inhibitors of Streptococcus sanguis OMZ 9 and Streptococcus sobrinus OMZ 176 adhesion to S-HA, whereas neither bovine serum albumin nor polyethyleneglycol were able to interfere with the adhesion of these strains. By contrast, none of the molecular species tested was able to inhibit the attachment of Actinomyces viscosus Ny 1 to S-HA. On the other hand, casein derivatives were shown to displace human serum albumin from S-HA beads. They were also able to bind to the bacterial cell surface of all strains examined. Collectively, these findings suggest that interactions between acidic casein-derived milk components and the biological surfaces involved in bacterial adhesion to S-HA result in an inhibitory effect that is selective for the oral streptococci examined.

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Section: Discussionmentioning

confidence: 96%

In vitro modulation of oral bacterial adhesion to saliva‐coated hydroxyapatite beads by milk casein derivatives

Neeser

Golliard

Woltz

et al. 1994

Oral Microbiology and Immunology

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“…Possible mechanisms, which may lead to root surface colonization by cariogenic organisms, have been studied in vitro. Streptococcus mutans serotype c is the most cotnmon species of mutans streptococci encountered in human plaque, and Gibbons et al have indicated that this organistn can attach to experitnental salivary pellicles (17,27). Salivary mucins and the fatnily of acidic proline-rich proteins (PRP) adsorbed onto apatitic surfaces have been shown to promote their attachment (17).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the distribution of Actinomyces in dental plaque on inserted enamel and natural tooth surfaces in periodontal health and disease

Liljemark

Bloomquist

Bandt

et al. 1993

Oral Microbiology and Immunology

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The distribution of Actinomyces naeslundii, Actinomyces viscosus and Actinomyces odontolyticus in healthy and diseased adult populations was studied in 3 different ways. First, supragingival plaque formation at 2 through 72 h was examined in 12 periodontally healthy adults using a removable pre-measured surface of enamel bonded to molars and premolars. Second, a cross-sectional examination of the composition of both supragingival and subgingival plaque of unknown age was conducted in 205 adults exhibiting periodontal health to moderate disease. Third, the effects of oral hygiene instruction and root planing on the subgingival microflora of a subset of 19 subjects with moderate periodontitis were examined. The evaluation of 12 adults revealed that the predominant species in early plaque formation (2, 4 and 8 h) was A. odontolyticus. A. viscosus and A. naeslundii were present in developing plaques in almost all subjects in 2-h plaque, but absent in half the subjects when 4-, 8- or 24-h plaque was examined. These two species significantly increased in numbers per mm2 enamel surface area in 48- and 72-h plaques. A. odontolyticus was not related to clinical signs of periodontal disease in 205 adults, and its subgingival proportions in plaque did not change following periodontal treatment of 19 individuals. A. naeslundii was found in significantly higher numbers in supragingival than subgingival plaques in the 205 adults examined. The mean proportion of A. naeslundii significantly decreased as the magnitude of probing depth and attachment loss increased. The proportions of A. naeslundii and A. viscosus were found to be significantly increased in subgingival plaques following periodontal treatment.

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“…Such an interaction would facilitate the removal of these aggregated streptococci from the oral cavity by a nonimmune mechanism. Alternatively, the salivary agglutinin can be adsorbed onto a solid support such as hydroxyapatite beads to simulate a tooth surface coated by a salivary pellicle (18,21,34,39,40). In this situation, the streptococcal cell surface protein molecules have been reported to function as adhesins.…”

mentioning

confidence: 99%

Differentiation of salivary agglutinin-mediated adherence and aggregation of mutans streptococci by use of monoclonal antibodies against the major surface adhesin P1

et al. 1992

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The ability to adhere to salivary agglutinin-coated hydroxyapatite beads and to aggregate in the presence of fluid-phase salivary agglutinin was tested by using 25 isolates of mutans streptococci representing eight serotypes. Both adherence and aggregation activity correlated with expression of the Mr-185,000 cell surface antigen P1 on Streptococcus mutans serotype c, e, and f strains. In addition, it was shown that the P1 molecule itself served as the adhesin of S. mutans serotype c, since adherence was significantly inhibited by the presence of recombinant-specified Mr-150,000 P1. The ability of S. sobrinus strains to adhere or aggregate did not correlate with expression of the P1 cross-reactive antigen SpaA. There was also evidence for interaction with salivary agglutinin, as manifested by aggregation but not adherence of S. rattus serotype b, which does not express a P1 cross-reactive antigen. To understand the interaction of Pl with salivary agglutinin at the molecular level, a panel of 11 anti-Pt monoclonal antibodies was tested for inhibitory activity in adherence and aggregation inhibition assays. Overlapping, but not identical, subsets of monoclonal antibodies were found to inhibit adherence and aggregation, indicating that the interactions of P1 with salivary agglutinin which mediate these two phenomena are different. The localization of functional domains of P1 which may mediate the aggregation and adherence reactions is discussed.

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A human salivary protein which promotes adhesion of Streptococcus mutans serotype c strains to hydroxyapatite

Cited by 57 publications

References 47 publications

In vitro modulation of oral bacterial adhesion to saliva‐coated hydroxyapatite beads by milk casein derivatives

In vitro modulation of oral bacterial adhesion to saliva‐coated hydroxyapatite beads by milk casein derivatives

Comparison of the distribution of Actinomyces in dental plaque on inserted enamel and natural tooth surfaces in periodontal health and disease

Differentiation of salivary agglutinin-mediated adherence and aggregation of mutans streptococci by use of monoclonal antibodies against the major surface adhesin P1

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