A human T cell‐specific cDNA clone (YT16) encodes a protein with extensive homology to a family of protein‐tyrosine kinases

Koga, Yuichi; Caccia, Nicolette; Toyonaga, Barry; Spolski, Rosanne; Yanagi, Yusuke; Yoshikai, Yasunobu; Mak, Tak W.

doi:10.1002/eji.1830161229

Cited by 87 publications

(52 citation statements)

References 9 publications

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“…Figure 7 demonstrates that this is, in fact, the case. By using a probe complementary to sequences in the 5' untranslated region of the human lck cDNA YT16 (12), transcripts were easily identified in both normal thymus RNA and in RNA derived from the human T cell line Jurkat (Fig. 7A).…”

Section: Resultsmentioning

confidence: 99%

Structure of the murine lck gene and its rearrangement in a murine lymphoma cell line.

Garvin¹,

Pawar²,

Marth³

et al. 1988

Mol. Cell. Biol.

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The lck gene encodes a lymphocyte-specific protein-tyrosine kinase that is implicated in neoplastic transformation. We have determined the germ line organization of the murine lck gene and have isolated and characterized a rearranged lck allele in the murine lymphoma cell line LSTRA. The overall exon-intron organization of the normal lck gene is almost identical to that of avian c-src. In LSTRA DNA, an internally rearranged Moloney murine leukemia virus genome is interposed between two distinct promoters that normally generate lck transcripts differing only in 5' untranslated regions. The rearrangement appears to have been selected to permit splicing of transcripts that initiate from the Moloney virus promoter to an acceptor site located within the first exon 3' to the downstream promoter, thus generating an lck mRNA with a novel 5' untranslated region that may be more efficiently translated.

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Section: Resultsmentioning

confidence: 99%

Structure of the murine lck gene and its rearrangement in a murine lymphoma cell line.

Garvin¹,

Pawar²,

Marth³

et al. 1988

Mol. Cell. Biol.

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“…Briefly, oligonucleotide primers used for rat cytokine genes IFN-␥ and IL-10 were those described (7,16,17). GAPDH primers served as the internal control.…”

Section: Semiquantitive Rt-pcr For Cytokine Genesmentioning

confidence: 99%

DNA Vaccination Against Specific Pathogenic TCRs Reduces Proteinuria in Active Heymann Nephritis by Inducing Specific Autoantibodies

Walters

Knight

et al. 2003

The Journal of Immunology

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We have previously identified potential pathogenic T cells within glomeruli that use TCR encoding Vβ5, Vβ7, and Vβ13 in combination with Jβ2.6 in Heymann nephritis (HN), a rat autoimmune disease model of human membranous nephritis. Vaccination of Lewis rats with naked DNA encoding these pathogenic TCRs significantly protected against HN. Proteinuria was reduced at 6, 8, 10, and 12 wk after immunization with Fx1A (p < 0.001). Glomerular infiltrates of macrophages and CD8+ T cells (p < 0.005) and glomerular IFN-γ mRNΑ expression (p < 0.01) were also significantly decreased. DNA vaccination (DV) causes a loss of clonality of T cells in the HN glomeruli. T lymphocytes with surface binding of Abs were found in DNA vaccinated rats. These CD3+/IgG+ T cells expressed Vβ5 and Vβ13 that the DV encoded. Furthermore, FACS shows that these CD3+/IgG+ cells were CD8+ T cells. Analysis of cytokine mRNA expression showed that IL-10 and IFN-γ mRNA were not detected in these CD3+/IgG+ T cells. These results suggest that TCR DNA vaccination produces specific autoantibodies bound to the TCRs encoded by the vaccine, resulting in blocking activation of the specific T cells. In this study, we have shown that treatment with TCR-based DV, targeting previously identified pathogenic Vβ families, protects against HN, and that the mechanism may involve the production of specific anti-TCR Abs.

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“…Briefly, oligonucleotide primers used for rat cytokine genes IL-10, IL-4, IFN-␥, and TGF-␤ were those previously described (11)(12)(13). GAPDH gene primers served as the internal control.…”

Section: Semiquantitive Rt-pcr For Cytokine Genesmentioning

confidence: 99%

Infiltration of Canonical Vγ4/Vδ1 γδ T Cells in an Adriamycin-Induced Progressive Renal Failure Model

Ando

Watson

et al. 2001

The Journal of Immunology

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We have previously reported an infiltration of renal interstitial γδ T cells in Adriamycin-induced progressive glomerulosclerosis in the rat kidney. The TCR repertoire and sequences used by these γδ T cells have now been studied. Two injections of Adriamycin 14 days apart caused segmental glomerulosclerosis, massive interstitial infiltration of mononuclear cells, and end-stage renal failure. Flow cytometry of lymphocyte subpopulations with Abs to CD3, the γδ TCR, and the αβ TCR showed that γδ T cells as a proportion of CD3+ cells were increased in Adriamycin-treated kidneys (8.5 ± 5.4%), but not in lymph nodes (1.3 ± 0.4%). A semiquantitative score of glomerular damage (r = 0.65; p < 0.01) and creatinine (r = 0.62; p < 0.01) correlated significantly with the presence of γδ T cells. TCR Vγ repertoire analysis by RT-PCR and Southern blotting showed that Vγ2 was the dominant subfamily in lymph nodes, whereas Vγ4 became the predominant subfamily in advanced stages of the rat Adriamycin-treated kidney. Sequencing of the Vγ4-Jγ junctional region showed an invariant sequence. The amino acid sequence of the junctional region of the Vγ4 TCR was the same as the reported mouse canonical Vγ4 TCR sequence. Analysis of the kidney Vδ repertoire showed dominant expression of Vδ1, and sequencing again revealed the selective expression of a canonical Vδ1 gene. Semiquantitative RT-PCR for cytokine gene expression showed that γδ T cells from the kidneys expressed TGF-β, but not IL-4, IL-10, or IFN-γ. These results suggest that the predominant γδ T cells in the Adriamycin kidney use an invariant Vγ4/Vδ1 receptor.

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A human T cell‐specific cDNA clone (YT16) encodes a protein with extensive homology to a family of protein‐tyrosine kinases

Cited by 87 publications

References 9 publications

Structure of the murine lck gene and its rearrangement in a murine lymphoma cell line.

Structure of the murine lck gene and its rearrangement in a murine lymphoma cell line.

DNA Vaccination Against Specific Pathogenic TCRs Reduces Proteinuria in Active Heymann Nephritis by Inducing Specific Autoantibodies

Infiltration of Canonical Vγ4/Vδ1 γδ T Cells in an Adriamycin-Induced Progressive Renal Failure Model

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