A Hybrid Bioprinting Approach for Scale-Up Tissue Fabrication

Yu, Yancun; Zhang, Yahui; Özbolat, İbrahim T.

doi:10.1115/1.4028511

Cited by 75 publications

(55 citation statements)

References 25 publications

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“…Drop-on-demand (DOD) technologies for micro-droplet deposition have been widely used in various fields owing to high precision and automation [1][2][3][4][5]; applications range from additive manufacturing [6][7][8][9][10] and electronics applications [11,12] to emerging fields such as pharmacology [13], pathology [14], tissue engineering [15][16][17][18], and biosensor manufacturing [19]. For instance, DOD mode micro-droplet deposition has been employed for high-throughput screening (HTS) applications to increase the efficiency of drug screening and delivery [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

Zhao

Yan

Yao

et al. 2015

Journal of Manufacturing Science and Engineering

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AbstractsDrop-on-demand (DOD) micro-droplet formation and deposition play an important role in additive manufacturing, particularly in printing of 3D in vitro biological models for pharmacological and pathological studies, for tissue engineering and regenerative medicine applications, and for building of cell-integrated micro-fluidic devices. In development of a DOD based micro-droplet deposition process for 3D cell printing, the droplet formation, controlled on-demand deposition and at the single cell level, and most importantly, maintaining the viability and functionality of the cells during and after the printing, are all remaining to be challenged. This report presents our recent study on developing a novel DOD based micro-droplet deposition process for 3D Printing by utilization of an alternating viscous & inertial force jetting mechanism. The results include an analysis of droplet formation mechanism, the system configuration, and experimental study of the effects of process parameters on micro-droplet formation. Sodium Alginate solutions are used for micro-droplet formation and deposition. Key process parameters include actuation signal waveforms, nozzle dimensional features, and solution SUN A c c e p t e d M a n u s c r i p t N o t C o p y e d i t e dviscosity. Sizes of formed micro-droplets are examined by measuring the droplet diameter and velocity. Resultsshow that by utilizing a nozzle at a 45μm diameter, the size of the formed micro-droplets are in the range of 52~72μm in diameter and 0.4~2.0m/s in jetting speed, respectively. Reproducibility of the system is also examined and the results show that the deviation of the formed micro-droplet diameter and the droplet deposition accuracy are within 6% and 6.2μm range, respectively. Experimental results demonstrate a high controllability and precision for the developed DOD micro-droplet deposition system with a potential for precise cell printing.

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Section: Introductionmentioning

confidence: 99%

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

Zhao

Yan

Yao

et al. 2015

Journal of Manufacturing Science and Engineering

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“…Droplet-based bioprinting has several advantages and disadvantages with respect to other bioprinting techniques, including extrusion-based bioprinting (mechanical [105][106][107][108][109], pneumatic [110][111][112][113][114] or valve-based [115][116][117][118]) or laser-based bioprinting (stereolithography [119] and its modifications [120], laser-guidance direct writing [18,121] and laser-induced forward transfer [19,122,123]). …”

Section: A Comparative Evaluation: Droplet-based Bioprinting Versus Omentioning

confidence: 99%

“…Angiogenic sprouting is a step towards addressing that challenge [114]. Figure 7D shows sprouted micro-capillaries out of bioprinted human umbilical vein endothelial cells (HUVECs) transfected with green fluorescent protein (GFP) and between the vascular channels seeded with HUVECs transfected with red fluorescent protein (RFP), 14 days post-bioprinting.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

A comprehensive review on droplet-based bioprinting: Past, present and future

2016

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Droplet-based bioprinting (DBB) offers greater advantages due to its simplicity and agility with precise control on deposition of biologics including cells, growth factors, genes, drugs and biomaterials, and has been a prominent technology in the bioprinting community. Due to its immense versatility, DBB technology has been adopted by various application areas, including but not limited to, tissue engineering and regenerative medicine, transplantation and clinics, pharmaceutics and high-throughput screening, and cancer research. Despite the great benefits, the technology currently faces several challenges such as a narrow range of available bioink materials, bioprinting-induced cell damage at substantial levels, limited mechanical and structural integrity of bioprinted constructs, and restrictions on the size of constructs due to lack of vascularization and porosity. This paper presents a first-time review of DBB and comprehensively covers the existing DBB modalities including inkjet, electrohydrodynamic, acoustic, and micro-valve bioprinting. The recent notable studies are highlighted, the relevant bioink biomaterials and bioprinters are expounded, the application areas are presented, and the future prospects are provided to the reader.

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“…Co-axial extrusion technique is another novel printing method that has been actively investigated lately 9, 26, 100 . Co-axial extrusion is an application of micro-extrusion printing.…”

Section: Cell Printing Technologiesmentioning

confidence: 99%

“…utilized two types of extrusion nozzles for scale-up tissue fabrication: a co-axial nozzle for printing tubular vasculature; and a traditional micro-nozzle for printing tissue strands with cell aggregates 100 . Two units of cell printing platform were used to simultaneously control printing processes of two distinct materials.…”

Section: Cell Printing Technologiesmentioning

confidence: 99%

Printing of Three-Dimensional Tissue Analogs for Regenerative Medicine

Lee

Dai

2016

Ann Biomed Eng

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3-D cell printing, which can accurately deposit cells, biomaterial scaffolds and growth factors in precisely defined spatial patterns to form biomimetic tissue structures, has emerged as a powerful enabling technology to create live tissue and organ structures for drug discovery and tissue engineering applications. Unlike traditional 3-D printing that uses metals, plastics and polymers as the printing materials, cell printing has to be compatible with living cells and biological matrix. It is also required that the printing process preserves the biological functions of the cells and extracellular matrix, and to mimic the cell-matrix architectures and mechanical properties of the native tissues. Therefore, there are significant challenges in order to translate the technologies of traditional 3-D printing to cell printing, and ultimately achieve functional outcomes in the printed tissues. So it is essential to develop new technologies specially designed for cell printing and in-depth basic research in the bioprinted tissues, such as developing novel biomaterials specifically for cell printing applications, understanding the complex cell-matrix remodeling for the desired mechanical properties and functional outcomes, establishing proper vascular perfusion in bioprinted tissues, etc. In recent years, many exciting research progresses have been made in the 3-D cell printing technology and its application in engineering live tissue constructs. This review paper summarized the current development in 3-D cell printing technologies; focus on the outcomes of the live printed tissues and their potential applications in drug discovery and regenerative medicine. Current challenges and limitations are highlighted, and future directions of 3-D cell printing technology are also discussed.

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A Hybrid Bioprinting Approach for Scale-Up Tissue Fabrication

Cited by 75 publications

References 25 publications

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

Alternating Force Based Drop-on-Demand Microdroplet Formation and Three-Dimensional Deposition

A comprehensive review on droplet-based bioprinting: Past, present and future

Printing of Three-Dimensional Tissue Analogs for Regenerative Medicine

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