A Hydrogen Peroxide Biosensor Based on the Immobilization of the Highly Stable Royal Palm Tree Peroxidase (Roystonea regia) with Chitosan and Glutaraldehyde on Screen-printed Graphene Electrodes

Abstract

<p>This paper present the fabrication and electrochemical stud-ies of a screen-printed graphene electrode (SPGE) modified with Roy-al palm tree peroxidase (RPTP) in combination with chitosan (CS) and cross-linking of glutaraldehyde (GA) for detection of hydrogen perox-ide. Cyclic voltammograms in the presence of potassium ferrocyanide ([Fe(CN)6]3-/4-) as a redox species demonstrated an increasing of 50mA evidenced by the electron transfer process of SPGE modified with CS-GA-RPTP. The graphene modified electrode exhibits excellent electrocatalytic activity to the reduction of H2O2, with a linear response in the 100 mM to 5 mM concentration range and a detection limit of 87 mM. The new sensor based on the modification of graphene electrode with the high stable RPTP will provide a potential amperometric detection system for determination of H2O2 in real samples with some biomedical or environmental importance.</p>

Highly Sensitive Hydrogen Peroxide Biosensor Based on Tobacco Peroxidase Immobilized on p‐Phenylenediamine Diazonium Cation Grafted Carbon Nanotubes: Preventing Fenton‐like Inactivation at Negative Potential

“…Table 1 shows a comparison between the analytical characteristics of the proposed H 2 O 2 biosensor with previously reported modified electrodes based on different types of plant peroxidases and/or different electrochemical platforms operating in direct electron transfer mode. It is possible to observe that the proposed r‐TOP/ p ‐PD/MWCNTs/G based biosensor shows a wider linear range compared with respect to some of the considered electrode platforms, comparable detection limit, a good sensitivity and a lower response time compared to other H 2 O 2 biosensors previously reported in the literature [78–82] . Moreover, it should be highlighted the reduced overpotential applied to perform H 2 O 2 detection, which could potentially reduce the interfering effect of some electroactive molecules (some molecules can act as diffusing electron donors).…”

“…It is possible to observe that the proposed r-TOP/p-PD/MWCNTs/G based biosensor shows a wider linear range compared with respect to some of the considered electrode platforms, comparable detection limit, a good sensitivity and a lower response time compared to other H 2 O 2 biosensors previously reported in the literature. [78][79][80][81][82] Moreover, it should be highlighted the reduced overpotential applied to perform H 2 O 2 detection, which could potentially reduce the interfering effect of some electroactive molecules (some molecules can act as diffusing electron donors).…”

Rapid electrochemical detection of Staphylococcus aureus based on screen-printed gold electrodes modified with cysteine and Guinea grass (Panicum maximum) peroxidase

“…There was no appreciable change in the current reduction peak for the bare gold electrode and, as expected, when GGP sensed H 2 O 2 we observed a decrease in the reduction peak around -457 mV ( Figure 2B) thus demonstrating the role of PODs as an efficient biocatalyst. Most of the electrochemical biosensors based on plant peroxidases have shown similar potential for bioelectrocatalytic reduction of H 2 O 2 Castillo, et al, 2006;Villamizar, et al, 2016). These results suggest that the electrocatalytic reduction of H 2 O 2 by SPGE-cys-GGP has potential utility for monitoring the decrease in hydrogen peroxide concentration to detect catalase-positive bacteria.…”

Design of an Electrochemical Biosensor for the Quantification of H2O2 for Application to Cardiovascular Diseases

“…In table I, shows the results obtained from the electroanalytical parameters values for the different platforms of the biosensor for H2O2 quantification. These results were compared with other sensors dates repotted in the literature (9)(10)(11)(12)(13)(14).…”