A Hyperpolarization-Activated Proton Channel in Zebrafish Sperm

Abstract

revealed association of hERG1a, hERG1b, and SCN5A mRNAs and their corresponding proteins hERG1a, hERG1b and Na V 1.5, indicative of a cotranslational complex. Furthermore, the transcripts can be co-regulated in iPSC-CMs as indicated by a coordinate decrease of SCN5A, hERG1a and hERG1b transcript levels upon hERG1b-specific silencing. Whole-cell patch clamp revealed a corresponding reduction in the magnitude of the potassium I Kr and sodium I Na currents. The co-translational association and coregulation of transcripts may represent a general mechanism by which cardiac cells coordinate expression and thus activity of different ion channel types. This mechanism may shed new light on cardiac rhythm maintenance and arrhythmogenic disorders, as well as other ion channel-related diseases.