APhellinus baumiiExtract Reduces Obesity in High-Fat Diet-Fed Mice and Absorption of Triglyceride in Lipid-Loaded Mice

Abstract

This study evaluated the anti-obesity effects of Phellinus baumii extract (PBE) in high-fat diet (HFD)-fed mice. Male 8-week-old C57BL/6 mice were randomly divided into four groups: control, normal chow diet plus vehicle; HFD-control, high-fat plus vehicle; HFD plus orlistat (Xenical(®), Roche, Basel, Switzerland) (50 mg/kg); and HFD plus PBE (500 mg/kg). PBE was administered daily by oral gavage for 12 weeks. Oral administration of PBE (500 mg/kg) significantly reduced body weight gain, hepatic lipid concentrations, and fat accumulation in epididymal adipocytes compared with mice fed HFD alone (P < .05). mRNA expression of genes related to triglyceride (TG) synthesis was suppressed in the PBE groups, and fatty acid synthase activity was also significantly inhibited (P < .05). Furthermore, we evaluated the effect of PBE on TG absorption and detected marked reduction in TG absorption in Xenical- and PBE-treated mice compared with the control group (P < .05). To determine the active compound of PBE, fractionation was conducted, and interfungin A, davallialactone, and hypholomine B were identified as the main compounds. Among the three identified compounds, as a representative compound, davallialactone was also shown to suppress fat accumulation in an in vitro model system. These anti-obesity and hypolipidemic effects appear to be partly mediated by suppressing plasma and hepatic fat accumulation through the inhibition of enzymes associated with hepatic and intestinal lipid absorption and synthesis.

Antiatherogenic Effect of Antioxidant Polyphenols from <i>Phellinus baumii </i> in Apolipoprotein E-Deficient Mice

“…Regarding this, it has been reported that PBE shows hypolipidemic effects in the context of TG metabolism. It appears to be partly mediated by suppression of plasma TG accumulation through the inhibition of enzymes associated with intestinal TG absorption and synthesis [24]. From this, it was assumed that PBE might not have a regulatory role in cholesterol metabolism, indicating that the atherosclerosis inhibition effect of PBE is probably related to the antioxidant and anti-inflammatory activity without affecting the regulation of cholesterol.…”

Effects ofPhellinus spp. Extract on Alcohol Metabolic Enzymes in Alcohol-treated Rats

“…Mushrooms are not only a nutritious food, but also are a therapeutic medicine (Zjawiony, 2004;Lee et al, 2010;Wang et al, 2014). Of the wild mushrooms studied so far, P. baumii was reported for anti-obesity (Noh et al, 2011), free radical scavenging activity (Lee et al, 2010) and hypoglycemic (Hwang et al, 2005) functions.…”

Phellinus baumii ethyl acetate extract inhibits lipopolysaccharide-induced iNOS, COX-2, and proinflammatory cytokine expression in RAW264.7 cells

“…Mushrooms are valuable sources of biologically active compounds possessing anticancer [17,19], antiplatelet [25,26], antiobesity [22], and anti-inflammatory [23,26] properties. In particular, and most importantly, medicinal mushrooms represent an unlimited source of polysaccharides which are primarily modifiers of biological response where these polymers interact with the immune system to up-or downregulate specific aspects of the response of the host and this may result in various therapeutic effects [19,27].…”

“…The mushroom, P. baumii has shown inhibitory effects on pulmonary inflammation induced by LPS in rats [23]. Noh et al [22] reported the antiobesity effect of P. baumii extract in mice. Recently, we reported the antiplatelet activity of P. baumii methanol extract [25].…”

“…Pretreatment with P. baumii extracts showed an antiobesity effect [22] and reduction in the level of total white cells, neutrophils, and IL-1b in LPS-induced rat pulmonary inflammation [23]. Moreover, isolated polysaccharides from P. baumii significantly enhanced lymphocyte proliferations and suppressed LPSinduced NO production in RAW264.7 cells [24].…”