A immunosensor for the diagnosis of canine distemper virus infection using SPR and EIS

Abstract

Canine distemper virus (CDV) is a viral disease that affects dogs and many other carnivores. Clinical diagnosis of CDV is difficult due to the broad spectrum of signs that may be confounded with other respiratory and enteric diseases of dogs. Laboratory analysis is required to diagnose suspected cases. In this study, surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS) methodologies were developed for the detection of canine distemper virus simultaneously. The assay exhibited high specificity, as all the negative controls were not mistakenly detected. The CDV concentration was determined from successive injections into the apparatus, with a linear range from 1.1 to 116.0 ng mL À1 . The system exhibited good reproducibility with 4.5% variation between runs after regeneration of the coated surface with a solution of 0.1 M glycine-HCL (pH 3.0). The capacitance and resistance values of the modified interface were calculated from EIS data using an equivalent circuit. It was possible to measure CDV in highly concentrated viruses with good specificity and reproducibility.

Electrochemical aptamer-based biosensor developed to monitor PSA and VEGF released by prostate cancer cells

“…Real time monitoring of layer formation by SPR technique revealed a well disperse aptamer surface with density of 6.6 ng/mm 2 [30]. Subsequently, the surface was passivated through the backfilling technique, with MCH, which is a well-known methodology to work with thiolated DNA sensor surfaces [31].…”

Aptamer‐based Biosensor Developed to Monitor MUC1 Released by Prostate Cancer Cells

“…The results shown in Figure C evidence a typical voltammogram of gold surfaces presenting cathodic peak at +1.45 V and anodic peak at +0.5 V. These results indicate the presence of a well‐formed gold layer and of a good electron transfer; therefore, this gold layer can be used as working electrode in electrochemical biosensors. The surface density of aptamer monolayer was assessed by SPR instrument by real time monitoring of layer formation of 6.6 ng/mm 2 showing a well disperse layer with similar density to antibody based biosensors .…”

“…The chemical interactions between the thiolated aptamer and gold surface were analized using SPR (AutoLabSpringle, Eco Chemie, NL), in order to evaluate aptamer density over the electrode. Briefly, gold SPR discs were purchased from Autolab and were incubated with 1 μM aptamer solution and an increase in the plasmon resonance angle of 120 millidegrees corresponds to a deposition of 1 ng/mm 2 on top of the gold disc, the experimental set up is described in details elsewhere .…”

Surface Plasmon Resonance: Advances of Label-Free Approaches in The Analysis of Biological Samples

“…The concept of immobilizing lentiviral particles bearing the specific receptor allows the study of ligand interactions in the cell membrane in close to in vivo conditions. In the work by Basso et al [76] a sensor for detection of canine distemper virus was developed. The sensor was based on immobilization of antibody on the chip surface, and both SPR and electrochemical impedance spectroscopy methods were used.…”