A ingestão de prébioticos previne a malabsorção de ferro e anemia induzidas pela gastrectomia?: Estudo experimental em ratos

Abstract

headings -Prebiotics. Gastrectomy. Iron. Anemia. Rats.abstRact -Background -The ingestion of two prebiotics, galactooligosaccharide (GOS) and polydextrose (PDX), leads to an increase in iron absorption and promotes recovery from anemia in gastrectomized rats. Aim -To study whether GOS + PDX stimulate iron absorption in gastrectomized and normals rats. Methods -Rats were laparotomized (sham-operated control) and partially gastrectomized (Billroth II) in groups of 20 each. Animals from both treatments were subsequently fed a control diet (AIN-93M) or the same diet supplemented with GOS and PDX (25 g/Kg of diet each) for eight weeks. They were divided into four subgroups, i.e., sham-operated and gastrectomized without GOS + PDX, sham-operated and gastrectomized with GOS + PDX. Two rats died during the experiment. All gastrectomized rats received an intramuscular injection of vitamin B-12 every two weeks. Hematocrit (HCT) and hemoglobin concentration (HGB) were measured at the start and on day 30 and 56 days after the start of feeding. On the final day of the study, total blood was collected for determination of serum iron concentration. Results -In the diet with GOS + PDX, iron excretion in feces was significantly lower than without the prebiotics. Apparent iron absorption and serum iron was higher in the GOS + PDX fed groups (both sham operated and gastrectomized) than in the non-GOS + PDX fed groups. The HCT and HGB concentrations were significantly lower in gastrectomized rats than in the control group, however, GOS + PDX feeding improved HGB levels in this group. ConclusionThe association of the GOS + PDX increase absorption of iron in sham-operated and gastrectomized rats and still prevent postgastrectomy anemia.Resumo -Racional -Ratos gastrectomizados desenvolvem anemia, e a ingestão de galactooligossacarídeo (GOS) e a polidextrose (PDX) aumenta a absorção de ferro e promove a recuperação da anemia em ratos com deficiência de ferro. Objetivo -Avaliar se GOS e PDX estimulam a absorção de ferro em ratos gastrectomizados e normais. Métodos -Os ratos foram laparotomizadas (controle falso-operado) e parcialmente gastrectomizados (Billroth II) em grupos de 20 animais. Os animais de ambos os tratamentos foram posteriormente submetidos à dieta controle (AIN-93M) ou a mesma dieta suplementada com GOS e PDX (25 g/kg de dieta cada) durante oito semanas e divididos em quatro subgrupos: sham-operados e gastrectomizados sem GOS e PDX, sham-operados e gastrectomizados com GOS e PDX. Dois animais morreram durante o experimento. Todos os ratos gastrectomizados receberam uma injeção intramuscular de vitamina B-12 a cada duas semanas. Hematócrito (HCT) e concentração de hemoglobina (HGB) foram dosados no início e nos dias 30 e 56 dias após o início da alimentação. No último dia do estudo, o sangue total foi coletado para determinação da concentração de ferro sérico. Resultados -Na dieta com GOS e PDX a excreção de ferro nas fezes foi significativamente menor do que no grupo sem prebióticos. Absorção aparente de ferro e ferro sé...

Effect of fructo-oligosaccharide supplementation in soya beverage on the intestinal absorption of calcium and iron in newly weaned rats

“…The Hb and Ht concentrations after the 7 d of prebiotic ingestion presented higher Hb recovery in the groups that received cows’ milk and soya beverage (CMF and SBF). These results are in agreement with the literature, showing that prebiotics can be effective in the regeneration of Hb mass ( 17 , 42 , 43 ) . The evaluation of Fe absorption by apparent absorption and hepatic Fe content did not confirm the results obtained for Hb and Ht concentrations.…”

Intake of Polydextrose Alters Hematology and the Profile of Short Chain Fatty Acids in Partially Gastrectomized Rats

“…One week after surgery, gastrectomized rats began to receive vitamin B12 supplementation (Bedoze ® SM Register: 014/75, São Paulo, SP, Brazil). This was given intramuscularly at a dose of 0.5 mg/kg every 15 days until the end of the experiment [ 41 , 42 ], to prevent the development of megaloblastic anemia. The animals of the Sham group received 0.9% sodium chloride (Arboreto ® Ltda.-Lot: 15125715, Juiz de Fora, MG, Brazil) to simulate the same stress.…”

Influência da incorporação de polidextrose como substituto de gordura na qualidade de pão de queijo

“…As fibras possuem baixo valor calórico e a polidextrose, como já demonstrado em estudos, pode influenciar positivamente na absorção dos minerais cálcio, ferro, magnésio e fósforo, além de ajudar no controle do apetite, promovendo saciedade, o que a longo prazo poderia trazer benefícios à saúde, com o controle e a prevenção de doenças como a obesidade, diabetes mellitus tipo 2, hipertensão arterial e dislipidemias (Gibson et al, 2004;Santos et al, 2011;Tiihonen et al, 2011;Konings et al, 2014;Carmo et al, 2016).…”