A keratinocyte and integrated fibroblast culture model for studying particulate matter-induced skin lesions and therapeutic intervention of fucosterol

Fernando, Ilekuttige Priyan Shanura; Jayawardena, Thilina U.; Kim, Hyunsoo; Vaas, A.P.J.P.; Silva, H.I.C. De; Nanayakkara, C.M.; Abeytunga, D.T.U.; Lee, Won Woo; Ahn, Ginnae; Lee, Daesung; Yeo, In-Kyu; Jeon, You‐Jin

doi:10.1016/j.lfs.2019.116714

Cited by 43 publications

(42 citation statements)

References 24 publications

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“…It was shown that PM induces mitochondrial-dependent apoptosis, indicated by an increased permeability of the mitochondrial membrane [123,168,175,177,183,184], increased cellular cytochrome c [177], and activation of caspase-3, caspase-9 and poly (ADP-ribose) polymerase (PARP) [123,125,133,168,175,177,[182][183][184]186]. Furthermore, PM-induced apoptosis was observed by and upregulation of apoptosis regulator BAX, a downregulation of anti-apoptotic proteins Bcl-2 and Mcl-1 [123,168,175,182], DNA fragmentation [123,128,168,175,177,[182][183][184], and the formation of apoptotic bodies [123,137,168,172,175,177,[182][183][184]186]. ER stress is also related to autophagy, and PM exposure was shown to promote autophagy in vitro.…”

Section: Pm-induced Apoptosis and Autophagymentioning

confidence: 99%

“…This, in turn, initiates the signaling cascade, and upon activation of NF-κB, the p65 subunit translocates to the nucleus, where it regulates the expression of its target genes that are involved in various processes of the inflammatory response [241]. Indeed, PM induces the phosphorylation and nuclear translation of the p65 unit [128,130,136,137,145,169,179,181,231,233]. The PM-induced activation of NF-κB leads to the increased transcription of its target genes and their protein expressions such as IL-1α, IL-1β, IL-6, IL-8, ICAM1, COX2 and TNF-α [124,128,130,133,135,138,139,142,169,173,181,227,229,230,243].…”

Section: Pm-induced Activation Of Nf-κbmentioning

confidence: 99%

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Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Dijkhoff¹,

Drašler²,

Karakoçak³

et al. 2020

Part Fibre Toxicol

126

116

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Background: Air pollution is killing close to 5 million people a year, and harming billions more. Air pollution levels remain extremely high in many parts of the world, and air pollution-associated premature deaths have been reported for urbanized areas, particularly linked to the presence of airborne nano-sized and ultrafine particles. Main text: To date, most of the research studies did focus on the adverse effects of air pollution on the human cardiovascular and respiratory systems. Although the skin is in direct contact with air pollutants, their damaging effects on the skin are still under investigation. Epidemiological data suggested a correlation between exposure to air pollutants and aggravation of symptoms of chronic immunological skin diseases. In this study, a systematic literature review was conducted to understand the current knowledge on the effects of airborne particulate matter on human skin. It aims at providing a deeper understanding of the interactions between air pollutants and skin to further assess their potential risks for human health. Conclusion: Particulate matter was shown to induce a skin barrier dysfunction and provoke the formation of reactive oxygen species through direct and indirect mechanisms, leading to oxidative stress and induced activation of the inflammatory cascade in human skin. Moreover, a positive correlation was reported between extrinsic aging and atopic eczema relative risk with increasing particulate matter exposure.

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Section: Pm-induced Apoptosis and Autophagymentioning

confidence: 99%

Section: Pm-induced Activation Of Nf-κbmentioning

confidence: 99%

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Dijkhoff¹,

Drašler²,

Karakoçak³

et al. 2020

Part Fibre Toxicol

126

116

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“…Intracellular ROS levels were measured after incubating for an hour (DCFH2-DA assay) and cell viability was measured by MTT assay after incubating for 24 h [1]. SHC4 that indicated superior antioxidant and cytoprotective effects were further verified by DCFH2-DA staining of cells and analysis by fluorescence microscopy (EVOS FL Auto 2 Imaging, ThermoFisher Scientific, CA, USA) and flow cytometry (CytoFLEX, Beckman Coulter, PA, USA) based on methods described in our previous publications [8].…”

Section: Uvb Exposure and Oxidative Stress Analysismentioning

confidence: 99%

Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

et al. 2020

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Ultraviolet B (UVB) radiation-induced oxidative skin cell damage is a major cause of photoaging. In the present study, a low molecular weight fucoidan fraction (SHC4) was obtained from Sargassum horneri by Celluclast-assisted extraction, followed by step gradient ethanol precipitation. The protective effect of SHC4 was investigated in human keratinocytes against UVB-induced oxidative stress. The purified fucoidan was characterized by Fourier-transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (NMR), agarose gel-based molecular weight analysis and monosaccharide composition analysis. SHC4 had a mean molecular weight of 60 kDa, with 37.43% fucose and 28.01 ± 0.50% sulfate content. The structure was mainly composed of α-L-Fucp-(1→4) linked fucose units. SHC4 treatment dose-dependently reduced intracellular reactive oxygen species (ROS) levels and increased the cell viability of UVB exposed HaCaT keratinocytes. Moreover, SHC4 dose-dependently inhibited UVB-induced apoptotic body formation, sub-G1 accumulation of cells and DNA damage. Inhibition of apoptosis was mediated via the mitochondria-mediated pathway, re-establishing the loss of mitochondrial membrane potential. The UVB protective effect of SHC4 was facilitated by enhancing intracellular antioxidant defense via nuclear factor erythroid 2–related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling. Further studies may promote the use of SHC4 as an active ingredient in cosmetics and nutricosmetics.

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“…Although several studies recommend the use of cell lines in this kind of experiments [66][67][68][69][70][71], it is of paramount importance to indicate that in vitro studies have evidenced that in a standard cell culture, fibroblast positively influence keratinocyte growth, most likely due to the fact that these cells secrete soluble growth factors. In natural skin, the interaction is relevant as well.…”

Section: Histological Analysismentioning

confidence: 99%

“…The amniotic membrane has structures, which are histologically similar to the skin, i.e., composed of a multilayer epithelium and the basic membrane, and the structure might be considered as a good support for wound healing, reepithelialization and inhibition of scar formation and bacterial growth [50,52,75,76]. Although several studies recommend the use of cell lines in this kind of experiments [66][67][68][69][70][71], it is of paramount importance to indicate that in vitro studies have evidenced that in a standard cell culture, fibroblast positively influence keratinocyte growth, most likely due to the fact that these cells secrete soluble growth factors. In natural skin, the interaction is relevant as well.…”

Section: Histological Analysismentioning

confidence: 99%

Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin

et al. 2020

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Decellularized membranes (DM) were obtained from bovine amniotic membranes (BAM) using four different decellularization protocols, based on physical, chemical, and mechanical treatment. The new material was used as a biological scaffold for in vitro skin cell culture. The DM were characterized using hematoxylin-eosin assay, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR-ATR), and differential scanning calorimetry (DSC). The in vitro cytotoxicity of DM was evaluated using MTT. The efficacy of decellularization process was assessed through DNA quantification and electrophoresis. All the used protocols showed a high effectiveness in terms of elimination of native cells, confirmed by DNA extraction and quantification, electrophoresis, and SEM, although protocol IV removes the cellular contents and preserve the native extracellular matrix (ECM) architecture which it can be considered as the most effective in terms of decellularization. FTIR-ATR and DSC on the other hand, revealed the effects of decellularization on the biochemical composition of the matrices. There was no cytotoxicity and the biological matrices obtained were a source of collagen for recellularization. The matrices of protocols I, II, and III were degraded at day 21 of cell culture, forming a gel. The biocompatibility in vitro was demonstrated; hence these matrices may be deemed as potential scaffold for epithelial tissue regeneration.

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A keratinocyte and integrated fibroblast culture model for studying particulate matter-induced skin lesions and therapeutic intervention of fucosterol

Cited by 43 publications

References 24 publications

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies

Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin

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