A Kinetic Study of Analyte–Receptor Binding and Dissociation, and Dissociation Alone, for Biosensor Applications: A Fractal Analysis

A Mathematical Analysis Using Fractals for Binding Interactions of Nuclear Estrogen Receptors Occurring on Biosensor Surfaces

“…The details are not repeated here, except that just the equations are given to permit an easier reading. These equations have been applied to other biosensor systems (14,17). Here we will attempt to apply these equations to (a) the influence of two different ligands on the binding of and dissociation rate coefficients for the interaction between clone 31 protein immobilized on the surface of a CM5 sensor chip and ER␣ in solution (10), (b) influence of different ligands on the binding and dissociation rate coefficients for different analyte-nuclear receptors on a SPR biosensor (11), and (c) binding and dissociation rate coefficients for different concentrations of ER␣ in solution to GST-DRIP immobilized on a sensor chip surface (15).…”

“…The details of the theory and the equations involved for the binding and the dissociation phases for analyte-receptor binding are available (14). The details are not repeated here, except that just the equations are given to permit an easier reading.…”

“…Fractals are disordered systems and the disorder is described by nonintegral dimensions (12). Fractals have previously been used to analyze the binding and dissociation kinetics of a variety of analyte-receptor systems (13,14). Fractals are particularly useful for this type of analysis because they help characterize the heterogeneity that exists on the surface by a lumped parameter, the fractal dimension.…”

Fractal binding and dissociation kinetics of lecithin cholesterol acyl transferase (LCAT), a heart-related compound, on biosensor surfaces

“…These equations have been applied to other biosensor systems. 12,13 For most applications, a single-or a dual-fractal analysis is often adequate to describe the binding and the dissociation kinetics. Peculiarities in the values of the binding and the dissociation rate coefficients, as well as in the values of the fractal dimensions with regard to the dilute analyte systems being analyzed will be carefully noted, if applicable.…”

SPR: Uma nova ferramenta para biossensores

“…A técnica de SPR é uma ferramenta poderosa para este fim, pois as análises podem ser feitas em tempo real. Análises cinéticas para avaliação e determinação de constantes de afinidades (associação, dissociação), ordem de reação, entre outros, foram apresentadas em diversos artigos [63][64][65][66][67][68][69][70][71][72][73] . Vários tipos de imunossensores, biossensores e sensores já foram desenvolvidos, utilizando SPR como elemento de transdução [74][75][76][77][78][79][80] .…”