A Knitted Scaffold for Tendon Engineering Using Poly (Lactic Acid) Fibers

Guo, Zheng; Chen, Jin Jing; Zhang, Pei Hua

doi:10.4028/www.scientific.net/amr.197-198.164

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…4 Ideal tendon tissue engineering scaffold materials should exhibit good biocompatibility and biodegradability, strong mechanical properties, high porosity, adjustable pore sizes, and the ability to mimic the basic structures and ECM environments of native tendons to promote cell growth and tissue formation. 5,6 Both natural (collagen, 7 chitin, 8 and silk 9 ) and biodegradable synthetic [poly(lactic acid), 10 poly(lactide-co-glycolide), 11 and poly (glycolic acid) 12 ] materials have been used as fibrous scaffolds for tendon regeneration. Although past studies have produced promising results, the scaffold architectures differ from the structure and inherent nanoscale organization of native tendons.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Electrospun Poly(L-Lactide-co-ɛ-Caprolactone)/Collagen Nanoyarn Network as a Novel, Three-Dimensional, Macroporous, Aligned Scaffold for Tendon Tissue Engineering

Wang

et al. 2013

Tissue Engineering Part C: Methods

111

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Tissue engineering techniques using novel scaffolding materials offer potential alternatives for managing tendon disorders. An ideal tendon tissue engineered scaffold should mimic the three-dimensional (3D) structure of the natural extracellular matrix (ECM) of the native tendon. Here, we propose a novel electrospun nanoyarn network that is morphologically and structurally similar to the ECM of native tendon tissues. The nanoyarn, random nanofiber, and aligned nanofiber scaffolds of a synthetic biodegradable polymer, poly(l-lactide-co-ecaprolactone) [P(LLA-CL)], and natural collagen I complex were fabricated using electrospinning. These scaffolds were characterized in terms of fiber morphology, pore size, porosity, and chemical and mechanical properties for the purpose of culturing tendon cells (TCs) for tendon tissue engineering. The results indicated a fiber diameter of 632 -81 nm for the random nanofiber scaffold, 643 -97 nm for the aligned nanofiber scaffold, and 641 -68 nm for the nanoyarn scaffold. The yarn in the nanoyarn scaffold was twisted by many nanofibers similar to the structure and inherent nanoscale organization of tendons, indicating an increase in the diameter of 9.51 -3.62 mm. The nanoyarn scaffold also contained 3D aligned microstructures with large interconnected pores and high porosity. Fourier transform infrared analyses revealed the presence of collagen in the three scaffolds. The mechanical properties of the sample scaffolds indicated that the scaffolds had desirable mechanical properties for tissue regeneration. Further, the results revealed that TC proliferation and infiltration, and the expression of tendon-related ECM genes, were significantly enhanced on the nanoyarn scaffold compared with that on the random nanofiber and aligned nanofiber scaffolds. This study demonstrates that electrospun P(LLA-CL)/collagen nanoyarn is a novel, 3D, macroporous, aligned scaffold that has potential application in tendon tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Electrospun Poly(L-Lactide-co-ɛ-Caprolactone)/Collagen Nanoyarn Network as a Novel, Three-Dimensional, Macroporous, Aligned Scaffold for Tendon Tissue Engineering

Wang

et al. 2013

Tissue Engineering Part C: Methods

111

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“…The applicable techniques include, for example, non-woven methods 6 - 8 and knitting 9 - 14 to produce porous structures for guided tissue ingrowth and tissue engineering purposes. Though textiles can be used as such, they can also be used as preforms to prepare three-dimensional structures, like scaffolds, e.g., for bone reconstruction 15 and for small joint reconstructions 16 - 19 …”

Section: Introductionmentioning

confidence: 99%

Knitted polylactide 96/4 L/D structures and scaffolds for tissue engineering: Shelf life, in vitro and in vivo studies

Ellä

Annala²,

Länsman

et al. 2011

Biomatter

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This study covers the whole production cycle, from biodegradable polymer processing to an in vivo tissue engineered construct. Six different biodegradable polylactide 96/4 L/D single jersey knits were manufactured using either four or eight multifilament fiber batches. The properties of those were studied in vitro for 42 weeks and in 0- to 3-year shelf life studies. Three types (Ø 12, 15 and 19 mm) of cylindrical scaffolds were manufactured from the knit, and the properties of those were studied in vitro for 48 weeks. For the Ø 15 mm scaffold type, mechanical properties were also studied in a one-year in vivo experiment. The scaffolds were implanted in the rat subcutis. All the scaffolds were g-irradiated prior to the studies. In vitro, all the knits lost 99% of their mechanical strength in 30 weeks. In the three-year follow up of shelf life properties, there was no decrease in the mechanical properties due to the storage time and only a 12% decrease in molecular weight. The in vitro and in vivo scaffolds lost their mechanical properties after 1 week. In the case of the in vivo samples, the mechanical properties were restored again, stepwise, by the presence of growing/maturing tissue between weeks 3 and 12. Faster degradation was observed with in vitro scaffolds compared to in vivo scaffolds during the one-year follow up.

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“…During the last two decades, many biodegradable structures were elaborated and proposed as scaffold for replacing tissues such as ligaments and tendons [3,[5][6][7][8]. Nevertheless, to authors' knowledge few studies have investigated the time dependency of scaffolds' mechanical properties evolution in culture conditions in vitro and/or in vivo [9][10][11]. Indeed, usually when degradation is investigated, it is either polymer alone or a scaffold structure in phosphate buffer solution (PBS) conditions without changing the culture medium every two or three days [12][13][14].…”

mentioning

confidence: 98%

Mechanical properties evolution of a PLGA-PLCL composite scaffold for ligament tissue engineering under static and cyclic traction-torsionin vitroculture conditions

Kahn

Ziani

Zhang

et al. 2012

Journal of Biomaterials Science, Polymer Edition

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This study aims to investigate the in vitro degradation of a poly(L-lactic-co-glycolic acid)-poly(L-lactic-co-ϵ-caprolactone) (PLGA-PLCL) composite scaffold's mechanical properties under static culture condition and 2 h period per day of traction-torsion cyclic culture conditions of simultaneous 10% uniaxial strain and 90° of torsion cycles at 0.33 Hz. Scaffolds were cultured in static conditions, during 28 days, with or without cell seeded or under dynamic conditions during 14 days in a bioreactor. Scaffolds' biocompatibility and proliferation were investigated with Alamar Blue tests and cell nuclei staining. Scaffolds' mechanical properties were tested during degradation by uniaxial traction test. The PLGA-PLCL composite scaffold showed a good cytocompatibility and a high degree of colonization in static conditions. Mechanical tests showed a competition between two process of degradation which have been associated to hydrolytic and enzymatic degradation for the reinforce yarn in poly(L-lactic-co-glycolic acid) (PLGA). The enzymatic degradation led to a decrease effect on mechanical properties of cell-seeded scaffolds during the 21st days, but the hydrolytic degradation was preponderant at day 28. In conclusion, the structure of this scaffold is adapted to culture in terms of biocompatibility and cell orientation (microfiber) but must be improved by delaying the degradation of it reinforce structure in PLGA.

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A Knitted Scaffold for Tendon Engineering Using Poly (Lactic Acid) Fibers

Cited by 4 publications

References 10 publications

Fabrication of Electrospun Poly(L-Lactide-co-ɛ-Caprolactone)/Collagen Nanoyarn Network as a Novel, Three-Dimensional, Macroporous, Aligned Scaffold for Tendon Tissue Engineering

Fabrication of Electrospun Poly(L-Lactide-co-ɛ-Caprolactone)/Collagen Nanoyarn Network as a Novel, Three-Dimensional, Macroporous, Aligned Scaffold for Tendon Tissue Engineering

Knitted polylactide 96/4 L/D structures and scaffolds for tissue engineering: Shelf life, in vitro and in vivo studies

Mechanical properties evolution of a PLGA-PLCL composite scaffold for ligament tissue engineering under static and cyclic traction-torsionin vitroculture conditions

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