A label-free and high-efficient GO-based aptasensor for cancer cells based on cyclic enzymatic signal amplification

Xiao, Kunyi; Liu, Juan; Chen, Hui; Zhang, Song; Kong, Jilie

doi:10.1016/j.bios.2016.11.057

Cited by 47 publications

(10 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the non-specific displacement caused by other molecules and incomplete interaction between aptamers and cells reduced the sensitivity of this method. For this view, they further developed a GO-based cytosensor through cyclic enzymatic signal amplification [91]. As shown in Figure 3B, hairpin aptamers were firstly bound to cells and initiated nicking endonuclease-assisted signal amplification.…”

Section: Nanoquencher-based Fluorescent Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

View full text Add to dashboard Cite

The accurate analysis of circulating tumor cells (CTCs) holds great promise in early diagnosis and prognosis of cancers. However, the extremely low abundance of CTCs in peripheral blood samples limits the practical utility of the traditional methods for CTCs detection. Thus, novel and powerful strategies have been proposed for sensitive detection of CTCs. In particular, nanomaterials with exceptional physical and chemical properties have been used to fabricate cytosensors for amplifying the signal and enhancing the sensitivity. In this review, we summarize the recent development of nanomaterials-based optical and electrochemical analytical techniques for CTCs detection, including fluorescence, colorimetry, surface-enhanced Raman scattering, chemiluminescence, electrochemistry, electrochemiluminescence, photoelectrochemistry and so on.

show abstract

Section: Nanoquencher-based Fluorescent Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

View full text Add to dashboard Cite

show abstract

“…This study used aptamer-carbon dot probes to detect CA125-positive cells and measured the FRET signals caused by the interaction of the carbon dots and AuNPs, which acted as nanoquenchers [ 87 ]. Xiao et al used graphene-oxide (GO), which binds to single-stranded DNA, as a FRET quencher in a similar manner [ 88 ]. When the analyte was not present, fluorescent labels on the aptamer were brought in close proximity to GO and the signal was quenched, providing very low background signal.…”

Section: Recent Progress In Aptamer-based Biosensor Technologymentioning

confidence: 99%

Current Advances in Aptamers for Cancer Diagnosis and Therapy

Hori

Herrera

Rossi

et al. 2018

Cancers

143

103

View full text Add to dashboard Cite

Nucleic acid aptamers are single-stranded oligonucleotides that interact with target molecules with high affinity and specificity in unique three-dimensional structures. Aptamers are generally isolated by a simple selection process called systematic evolution of ligands by exponential enrichment (SELEX) and then can be chemically synthesized and modified. Because of their high affinity and specificity, aptamers are promising agents for biomarker discovery, as well as cancer diagnosis and therapy. In this review, we present recent progress and challenges in aptamer and SELEX technology and highlight some representative applications of aptamers in cancer therapy.

show abstract

“…Graphene oxide (GO) has been reported to adsorb single-stranded nucleic acid chains by cooperative van der Waals' force, π-π stacking interaction, and hydrogen bonding interaction (Antony and Grimme, 2008; Varghese et al, 2009; Wu et al, 2011; Chen et al, 2014; Liu et al, 2016). Recently, some aptamer-adsorbed GO have been demonstrated for protein detection and cell labeling (Pu et al, 2011; Gao et al, 2015; Kim et al, 2015; Xiao et al, 2017). Most aptamers adopt specific conformational structures, which enable high specificity toward the targeting molecule (Rowsell et al, 1998; Tucker et al, 2012; Zavyalova et al, 2016b,c; Krauss et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Supramolecular Aptamers on Graphene Oxide for Efficient Inhibition of Thrombin Activity

et al. 2019

View full text Add to dashboard Cite

Graphene oxide (GO), a two-dimensional material with a high aspect ratio and polar functional groups, can physically adsorb single-strand DNA through different types of interactions, such as hydrogen bonding and π-π stacking, making it an attractive nanocarrier for nucleic acids. In this work, we demonstrate a strategy to target exosites I and II of thrombin simultaneously by using programmed hybrid-aptamers for enhanced anticoagulation efficiency and stability. The targeting ligand is denoted as Supra-TBA 15/29 (supramolecular TBA 15/29 ), containing TBA 15 (a 15-base nucleotide, targeting exosite I of thrombin) and TBA 29 (a 29-base nucleotide, targeting exosite II of thrombin), and it is designed to allow consecutive hybridization of TBA 15 and TBA 29 to form a network of TBAs (i.e., supra-TBA 15/29 ). The programmed hybrid-aptamers (Supra-TBA 15/29 ) were self-assembled on GO to further boost anticoagulation activity by inhibiting thrombin activity, and thus suppress the thrombin-induced fibrin formation from fibrinogen. The Supra-TBA 15/29 -GO composite was formed mainly through multivalent interaction between poly(adenine) from Supra-TBA 15/29 and GO. We controlled the assembly of Supra-TBA 15/29 on GO by regulating the preparation temperature and the concentration ratio of Supra-TBA 15/29 to GO to optimize the distance between TBA 15 and TBA 29 units, aptamer density, and aptamer orientation on the GO surfaces. The dose-dependent thrombin clotting time (TCT) delay caused by Supra-TBA 15/29 -GO was >10 times longer than that of common anticoagulant drugs including heparin, argatroban, hirudin, and warfarin. Supra-TBA 15/29 -GO exhibits high biocompatibility, which has been proved by in vitro cytotoxicity and hemolysis assays. In addition, the thromboelastography of whole-blood coagulation and rat-tail bleeding assays indicate the anticoagulation ability of Supra-TBA 15/29 -GO is superior to the most widely used anticoagulant (heparin). Our highly biocompatible Supra-TBA 15/29 -GO with strong multivalent interaction with thrombin [dissociation constant ( K d ) = 1.9 × 10 −11 M] shows great potential as an effective direct thrombin inhibitor for the treatment of hemostatic disorders.

show abstract

A label-free and high-efficient GO-based aptasensor for cancer cells based on cyclic enzymatic signal amplification

Cited by 47 publications

References 37 publications

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

Current Advances in Aptamers for Cancer Diagnosis and Therapy

Supramolecular Aptamers on Graphene Oxide for Efficient Inhibition of Thrombin Activity

Contact Info

Product

Resources

About