A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer

Liu, Feng; Ding, Ailing; Zheng, Jiushang; Chen, Jiucun; Wang, Bin

doi:10.3390/s18061769

Cited by 30 publications

(20 citation statements)

References 37 publications

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“…This presented MNPs-SPEs sensor offers higher sensitivity as compared with other methods in the detection of OTA, including fluorescence methods [ 33 , 34 , 35 ], enzyme immunoassay [ 30 ], fluorescence polarization (FP) analysis [ 36 ], and other different sensor methods [ 37 , 38 , 39 , 40 , 41 ]. Compared with other ultrasensitive techniques, such as fluorescence resonance energy transfer (FRET) aptasensor [ 42 ] and impedimetric aptasensor [ 43 ], our MNPs-SPEs sensor offers a similar detection limit, but in a shorter detection time.…”

Section: Resultsmentioning

confidence: 99%

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Zhang

Wang

Xie

et al. 2018

Toxins

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A rapid and sensitive electrochemical biosensor based on magnetic nanoparticles and screen-printed electrodes (MNPs-SPEs sensor) was developed for the detection of ochratoxin A (OTA) in cereal and feed samples. Different types of magnetic nanoparticles-based ELISA (MNPs-ELISA) were optimized, and the signal detection, as well as sensitivity, was enhanced by the combined use of screen-printed electrodes (SPEs). Under the optimized conditions, the calibration curve of the MNPs-SPEs sensor was y = 0.3372x + 0.8324 (R2 = 0.9805). The linear range of detection and the detection limit were 0.01–0.82 ng/mL and 0.007 ng/mL, respectively. In addition, 50% inhibition (IC50) was detectable at 0.10 ng/mL. The limit of detection (LOD) of this MNPs-SPEs sensor in cereal and feed samples was 0.28 μg/kg. The recovery rates in spiked samples were between 78.7% and 113.5%, and the relative standard deviations (RSDs) were 3.6–9.8%, with the coefficient of variation lower than 15%. Parallel analysis of commercial samples (corn, wheat, and feedstuff) showed a good correlation between MNPs-SPEs sensor and liquid chromatography tandem mass spectrometry (LC/MS-MS). This new method provides a rapid, highly sensitive, and less time-consuming method to determine levels of ochratoxin A in cereal and feedstuff samples.

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Section: Resultsmentioning

confidence: 99%

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Zhang

Wang

Xie

et al. 2018

Toxins

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show abstract

“…Highly sensitive assays for the detection of AFB 1 , OTA, ZEN, or FB 1 have been established by other researchers [ 39 , 40 , 41 , 42 , 43 , 44 ], but for the detection of co-existing mycotoxins, multiple quantitative test assays, such as the microarray immunoassay described herein, will be more efficient at satisfying the need of detection. The assay we devised can detect four different mycotoxins in one single run, which is a pivotal advantage of this new method.…”

Section: Discussionmentioning

confidence: 99%

Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Zhang

Wang

Fang

et al. 2018

Toxins

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We developed and tested a prototype of an antibody microarray immunoassay for simultaneous quantitative detection of four typical mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1) in corn samples. The test kit consisted of a nitrocellulose membrane layered with immobilized monoclonal antibodies against mycotoxins. During the assay, the mycotoxin-protein conjugates were biotinylated. The signal detection was enhanced by a combination of the biotin-streptavidin system and enhanced chemiluminescence (ECL). This improved the sensitivity of the assay. Under the optimized conditions, four calibration curves with goodness of fit (R2 > 0.98) were plotted. The results showed that the detection limits for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 were 0.21, 0.19, 0.09, and 0.24 ng/mL, with detection ranges of 0.47–55.69, 0.48–127.11, 0.22–31.36, and 0.56–92.57 ng/mL, respectively. The limit of detection (LOD) of this antibody microarray for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 in corn was 5.25, 4.75, 2.25, and 6 μg/kg, respectively. The recovery rates from the spiked samples were between 79.2% and 113.4%, with coefficient of variation <10%. The results of the analysis of commercial samples for mycotoxins using this new assay and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) were comparable and in good agreement. This assay could also be modified for the simultaneous detection of other multiple mycotoxins, as well as low-weight analytes, hazardous to human health.

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“…G‐quadruplex‐based sensing devices have also been used for the detection of small molecules,[91c], [91e], proteins and cells . The nucleotide ATP was detected using an aptamer‐forming DNA sequence that only assembled to a G‐quadruplex structure upon ATP binding.…”

Section: Ppix Derivatives For Chemical Biology Applicationsmentioning

confidence: 99%

“…A similar method was used for the detection of the mycotoxin ochratoxin A. G‐quadruplex formation upon binding of ochratoxin A to the DNA aptamer and recruitment of Zn(II)PPIX effected the fluorescent signal readout. [98a] A local surface plasmon resonance (LSPR)‐based ochratoxin sensor was designed that included DNA‐aptamers immobilized on LSPR‐active gold nanorods with Zn(II)PPIX again acting as signal enhancer. The aptamers were used to bring the plasmonic sensor and the analyte closer together, thereby improving the signal intensity.…”

Section: Ppix Derivatives For Chemical Biology Applicationsmentioning

confidence: 99%

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The Red Color of Life Transformed – Synthetic Advances and Emerging Applications of Protoporphyrin IX in Chemical Biology

Sitte

Senge

2020

Eur J Org Chem

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Protoporphyrin IX (PPIX) is the porphyrin scaffold of heme b, a ubiquitous prosthetic group of proteins responsible for oxygen binding (hemoglobin, myoglobin), electron transfer (cytochrome c) and catalysis (cytochrome P450, catalases, peroxidases). PPIX and its metallated derivatives frequently find application as therapeutic agents, imaging tools, catalysts, sensors and in light harvesting. The vast toolkit of accessible porphyrin functionalization reactions enables easy synthetic modification of PPIX to meet the requirements for its multiple uses. In the past few years, particular interest has arisen in exploiting the interaction of PPIX and its synthetic derivatives with biomolecules such as DNA and heme‐binding proteins to evolve molecular devices with new functions as well as to uncover potential therapeutic toeholds. This review strives to shine a light on the most recent developments in the synthetic chemistry of PPIX and its uses in selected fields of chemical biology.

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A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer

Cited by 30 publications

References 37 publications

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

The Red Color of Life Transformed – Synthetic Advances and Emerging Applications of Protoporphyrin IX in Chemical Biology

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