A label-free continuous total-internal-reflection-fluorescence-based immunosensor

Engström, Henrik A.; Andersson, Per Ola; Ohlson, Sten

doi:10.1016/j.ab.2006.03.058

Cited by 29 publications

(16 citation statements)

References 29 publications

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“…As a consequence, anti-carbohydrate polyclonal or monoclonal antibodies typically have poor affinity even though monoclonal antibody was used to build an immunosensor for the detection of panose (Glcα1-6Glcα1-4Glc), and maltose (Glcα1-4Glc) with a sensitivity in the range of 1-5 μM [102]. Lectins bind to cell surfaces through specific carbohydrate-containing receptor sites [103].…”

Section: Lectin-based Biosensors For Detecting Carbohydratesmentioning

confidence: 99%

Carbohydrate–protein interactions and their biosensing applications

Zeng

Andrade²,

Oliveira³

et al. 2011

Anal Bioanal Chem

105

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Carbohydrate recognition is clearly present throughout nature, playing a major role in the initial attachment of one biological entity to another. The important question is whether these prevalent interactions could provide a real suitable alternative to the use of antibodies or nucleic acid for detection and identification. Currently, examples of carbohydrates being employed in biological detection systems are limited. The challenges of using carbohydrate recognition for detection mainly come from the weak affinity of carbohydrate-protein interactions, the lack of versatile carbohydrate scaffolds with well-defined structures, and the less developed high-information-content, real-time, and label-free assay technology. In this review, we focus on discussing the characteristics of carbohydrate-protein interactions in nature and the methods for carbohydrate immobilization based on surface coupling chemistry in terms of their general applicability for developing carbohydrate- and lectin-based label-free sensors. Furthermore, examples of innovative design of multivalent carbohydrate-protein interactions for sensor applications are given. We limit our review to show the feasibility of carbohydrate and lectin as recognition elements for label-free sensor development in several representative cases to formulate a flexible platform for their use as recognition elements for real-world biosensor applications.

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Section: Lectin-based Biosensors For Detecting Carbohydratesmentioning

confidence: 99%

Carbohydrate–protein interactions and their biosensing applications

Zeng

Andrade²,

Oliveira³

et al. 2011

Anal Bioanal Chem

105

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show abstract

“…When further modification of GE with BSA (curve c), followed by antigen (curves dek) were carried out, the semicircle diameter of EIS in Nyquist plot increased steadily. This is the result of the increase in charge transfer resistance for redox probe to access the gold electrode (GE) surface, owing to the adsorption of bio-molecules which retards the electron transfer kinetics between the redox probe and the electrode surface [8]. The curves (dek) in Fig.…”

Section: Impedimetric Detection Of Antigeneantibody Interactionmentioning

confidence: 99%

“…Several techniques like chemiluminescence [1,2], surface plasmon resonance [3,4], quartz crystal microbalance [5,6], absorption and fluorescence spectroscopy [7,8] and electrochemical techniques [9,10], have been developed and are widely used in immunoassay.…”

Section: Introductionmentioning

confidence: 99%

Simple and sensitive electrochemical impedimetric approach towards analysis of biophysical interaction

Gupta

Mitra

2015

Biochemical and Biophysical Research Communications

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“…Molecules with fluorescence characteristics are excited in the evanescent field of the waveguide creating a fluorescent evanescent wave. This evanescent wave allows quantitative measurements of adsorbed molecules on the surface without influence of any moderate bulk concentration of the same analyte (Engström et al, 2006). The advantages of the format are its robustness, availability, versatility, low cost, and portability, which allow it to be used in the field, while its main drawback is the need for labels (González-Martínez et al, 2006).…”

Section: Total Internal Reflection Fluorescence (Tirf)mentioning

confidence: 99%