Simple and sensitive electrochemical impedimetric approach towards analysis of biophysical interaction

Gupta, Ajay Kumar; Mitra, Chanchal K.

doi:10.1016/j.bbrc.2015.08.041

Cited by 7 publications

(2 citation statements)

References 26 publications

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“…In general, R et increases when a biomolecule is immobilized on the electrode surface because it hinders the electron transfer. 49 The Nyquist plots were investigated from 0.1 to 10 6 Hz at 0.27 V as shown in Figure 4C. The bare GCE (curve a) displays a large R et value.…”

Section: Resultsmentioning

confidence: 99%

Green Synthesis of Reduced Graphene Oxide Decorated with Few-Layered MoS₂-Nanoroses and Au/Pd/Ag Trimetallic Nanoparticles for Ultrasensitive Label-Free Immunosensing Platforms

Sharifuzzaman

Barman

Zahed

et al. 2019

J. Electrochem. Soc.

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The conventional synthetic methods for graphene oxide are based on the reaction of graphite with mixed strong oxidants, which involves the risk of explosion and toxic gases. Herein, we report a facile, safe, and green method to synthesize green graphene oxide (denoted as gGO) using sodium periodate as an effective oxidizing agent feature with lower Raman intensity (I D /I G = 0.65) and higher C/O atomic ratio (∼5.15). We also discuss the mechanism underlying the direct growth of MoS 2 nanoroses (MoS 2 -NRs) on the in situ synthesized green reduced graphene oxide (grGO), in which the MoS 2 -NRs are assembled from few-layered MoS 2 nanosheets. Due to the highly exposed active edge sites of MoS 2 -NRs and good electrical coupling with the underlying grGO, the resulting grGO/MoS 2 -NRs nanocomposite exhibits excellent electrocatalytic activity. Furthermore, the addition of L-proline assisted Au/Pd/Ag trimetallic nanoparticles to the nanocomposite provide a suitable label-free platform for direct antibody immobilization and increase the effective surface area. The as-prepared grGO/MoS 2 -NRs/AuPdAg nanocomposite was employed as a label-free immunosensing platform for the ultrasensitive detection of ochratoxin A, a food mycotoxin. The proposed immunosensor exhibits an excellent high detection range from 10 fg mL −1 to 150 ng mL −1 and limit of detection (LOD) of 5 fg mL −1 (S/N = 3).

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Section: Resultsmentioning

confidence: 99%

Green Synthesis of Reduced Graphene Oxide Decorated with Few-Layered MoS₂-Nanoroses and Au/Pd/Ag Trimetallic Nanoparticles for Ultrasensitive Label-Free Immunosensing Platforms

Sharifuzzaman

Barman

Zahed

et al. 2019

J. Electrochem. Soc.

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“…Impedimetric sensors measure impedance, including resistance and capacitance parameters that originate from the result of an interaction with a small amplitude voltage signal as a function of frequency [16]. Measurements of R CT in antigen-antibody interaction analysis are more significant than measurements of current or potential changes [17]. …”

Section: Introductionmentioning

confidence: 99%

Fabrication of a Novel Highly Sensitive and Selective Immunosensor for Botulinum Neurotoxin Serotype A Based on an Effective Platform of Electrosynthesized Gold Nanodendrites/Chitosan Nanoparticles

Sorouri

Bagheri

Afkhami

et al. 2017

Sensors

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In this work, a novel nanocomposite consisting of electrosynthesized gold nanodendrites and chitosan nanoparticles (AuNDs/CSNPs) has been prepared to fabricate an impedimetric immunosensor based on a screen printed carbon electrode (SPCE) for the rapid and sensitive immunoassay of botulinum neurotoxin A (BoNT/A). BoNT/A polyclonal antibody was immobilized on the nanocomposite-modified SPCE for the signal amplification. The structure of the prepared nanocomposite was investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The charge transfer resistance (RCT) changes were used to detect BoNT/A as the specific immuno-interactions at the immunosensor surface that efficiently limited the electron transfer of Fe(CN)63−/4− as a redox probe at pH = 7.4. A linear relationship was observed between the %∆RCT and the concentration logarithm of BoNT/A within the range of 0.2 to 230 pg·mL−1 with a detection limit (S/N = 3) of 0.15 pg·mL−1. The practical applicability of the proposed sensor was examined by evaluating the detection of BoNT/A in milk and serum samples with satisfactory recoveries. Therefore, the prepared immunosensor holds great promise for the fast, simple and sensitive detection of BoNT/A in various real samples.

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Fabrication of a novel impedimetric sensor based on l-Cysteine/Cu(II) modified gold electrode for sensitive determination of ampyra

Hashemi

Afkhami

Bagheri

et al. 2017

Analytica Chimica Acta

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Simple and sensitive electrochemical impedimetric approach towards analysis of biophysical interaction

Cited by 7 publications

References 26 publications

Green Synthesis of Reduced Graphene Oxide Decorated with Few-Layered MoS₂-Nanoroses and Au/Pd/Ag Trimetallic Nanoparticles for Ultrasensitive Label-Free Immunosensing Platforms

Green Synthesis of Reduced Graphene Oxide Decorated with Few-Layered MoS₂-Nanoroses and Au/Pd/Ag Trimetallic Nanoparticles for Ultrasensitive Label-Free Immunosensing Platforms

Fabrication of a Novel Highly Sensitive and Selective Immunosensor for Botulinum Neurotoxin Serotype A Based on an Effective Platform of Electrosynthesized Gold Nanodendrites/Chitosan Nanoparticles

Fabrication of a novel impedimetric sensor based on l-Cysteine/Cu(II) modified gold electrode for sensitive determination of ampyra

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Simple and sensitive electrochemical impedimetric approach towards analysis of biophysical interaction

Cited by 7 publications

References 26 publications

Green Synthesis of Reduced Graphene Oxide Decorated with Few-Layered MoS2-Nanoroses and Au/Pd/Ag Trimetallic Nanoparticles for Ultrasensitive Label-Free Immunosensing Platforms

Green Synthesis of Reduced Graphene Oxide Decorated with Few-Layered MoS2-Nanoroses and Au/Pd/Ag Trimetallic Nanoparticles for Ultrasensitive Label-Free Immunosensing Platforms

Fabrication of a Novel Highly Sensitive and Selective Immunosensor for Botulinum Neurotoxin Serotype A Based on an Effective Platform of Electrosynthesized Gold Nanodendrites/Chitosan Nanoparticles

Fabrication of a novel impedimetric sensor based on l-Cysteine/Cu(II) modified gold electrode for sensitive determination of ampyra

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Green Synthesis of Reduced Graphene Oxide Decorated with Few-Layered MoS₂-Nanoroses and Au/Pd/Ag Trimetallic Nanoparticles for Ultrasensitive Label-Free Immunosensing Platforms

Green Synthesis of Reduced Graphene Oxide Decorated with Few-Layered MoS₂-Nanoroses and Au/Pd/Ag Trimetallic Nanoparticles for Ultrasensitive Label-Free Immunosensing Platforms