Fabrication of a novel impedimetric sensor based on l-Cysteine/Cu(II) modified gold electrode for sensitive determination of ampyra

Hashemi, Pegah; Afkhami, Abbas; Bagheri, Hasan; Amidi, Salimeh; Madrakian, Tayyebeh

doi:10.1016/j.aca.2017.06.038

Cited by 33 publications

(12 citation statements)

References 49 publications

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“…R CT evaluates the semicircle diameter domain at a higher frequency of the impedance spectra . For accurate estimation of R CT at the electrode surface, an equivalent circuit was used for fitting of the EIS and the obtained results from the Nyquist plot . The impedance spectra were fitted to the modified Randles equivalent circuit composed of the ohmic resistance of the electrolyte solution ( R s ) and the charge transfer resistance ( R CT ), in series with the diffusion-limited Warburg impedance ( W ) and in parallel to a constant phase element (CPE or Q ). , …”

Section: Results and Discussionmentioning

confidence: 99%

“…CV is a useful technique to probe the features of modified electrode surfaces. The changes in peak current along with the peak separation in CVs at various steps of surface modification are theoretically related to the electron transfer rate constant . To study the blocking behavior of various surface modifications, [Fe (CN) 6 ] 3–/4– was used as a redox probe.…”

Section: Results and Discussionmentioning

confidence: 99%

“…22 For accurate estimation of R CT at the electrode surface, an equivalent circuit was used for fitting of the EIS and the obtained results from the Nyquist plot. 30 The impedance spectra were fitted to the modified Randles equivalent circuit composed of the ohmic resistance of the electrolyte solution (R s ) and the charge transfer resistance (R CT ), in series with the diffusion-limited Warburg impedance (W) and in parallel to a constant phase element (CPE or Q). 30,31 Figure 3a illustrates the obtained Nyquist plots from various modification steps of the different impedimetric layers.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…30 The impedance spectra were fitted to the modified Randles equivalent circuit composed of the ohmic resistance of the electrolyte solution (R s ) and the charge transfer resistance (R CT ), in series with the diffusion-limited Warburg impedance (W) and in parallel to a constant phase element (CPE or Q). 30,31 Figure 3a illustrates the obtained Nyquist plots from various modification steps of the different impedimetric layers. The line through the data points in Figure 3a demonstrates the fitting curve of the modified Randles equivalent circuit.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Well-Orientation Strategy for Direct Immobilization of Antibodies: Development of the Immunosensor Using the Boronic Acid-Modified Magnetic Graphene Nanoribbons for Ultrasensitive Detection of Lymphoma Cancer Cells

et al. 2020

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This work presents an effective strategy for the well-oriented immobilization of antibodies in which boronic acid is directly attached to the surface and with no need of the long and flexible spacer. A magnetic graphene nanoribbon−boronic-acid-based immunosensor was developed and tested for the impedimetric detection of lymphoma cancer cells, a blood cancer biomarker. Magnetic graphene nanoribbons (MGNRs) were modified with boronic acid (BA) to create a supporting matrix that is utilized by immobilizing anti-CD20 antibodies with good orientation. The prepared biosensing layer (MGNR/BA/Ab) with well-oriented antibodies was premixed into whole blood samples to interact with lymphoma cancer cell receptors. In the presence of target cell receptors, an immunocomplex was formed between anti-CD20 antibodies and lymphoma cancer cell receptors. Then, the biosensing layer was magnetically collected on a screen-printed carbon electrode (SPCE) and placed in a homemade electrochemical cell configuration to measure impedimetric signals. The fabrication steps of the immunosensor were characterized by various techniques, such as resonance light scattering, fluorescence, electrochemical impedance spectroscopy, and cyclic voltammetry. The assay is highly sensitive: the calculated limit of detection of lymphoma cancer cells was as low as 38 cells/mL, and the detection was linear from 100 to 1 000 000 cells/mL. The specificity of the immunosensor is also very high, and there is no interference effect with several potential interferents, such as the breast cancer (MCF-7), human embryonic kidney (HEK293), and leukemia (HL-60 and KCL-22) cell lines. The performance of the immunosensor for lymphoma cancer cells in clinical blood samples is consistent with that of commercial flow cytometric assays.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

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Well-Orientation Strategy for Direct Immobilization of Antibodies: Development of the Immunosensor Using the Boronic Acid-Modified Magnetic Graphene Nanoribbons for Ultrasensitive Detection of Lymphoma Cancer Cells

et al. 2020

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“…As a powerful technique for probing the features of electrode surface, the EIS was applied to investigate the interfacial properties of electrode surface within the stepwise modication process. [34][35][36] . As it can be seen, signicant changes in the impedance data were obtained at bare GCE, PMel/GCE* and PMel-Au nano /GCE*.…”

Section: Eis Studiesmentioning

confidence: 99%

Sensitive electrochemical determination of rifampicin using gold nanoparticles/poly-melamine nanocomposite

et al. 2017

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Biofilm Formation in Water Supply Pipes

Xiao¹,

Waheed²

2019

Encyclopedia of Water

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Biofilm formation in water supply pipes is ubiquitous despite the practice of keeping a certain level of disinfectant residual. Biofilms in water supply pipes play a dominant role in shaping the microbial community and governing the microbial quality of tap water. However, this role has long been overlooked largely due to the unavailability of in situ biofilm monitoring tools and complexity of biofilm ecology. Recent development in molecular biology techniques has opened up a new window for biofilm studies, and thus sparked a new boom in research on biofilms in water supply pipes. This article introduces the new knowledge of occurrence and significance of biofilms in water supply pipes, their impacts on water quality and relationships with pipe corrosion. Then the factors affecting biofilm formation and methods to control it are elaborated. Furthermore, new insights into the microbial ecology of biofilms are presented, which include the dominant microorganisms and their potential roles in biofilms, the diversity and richness of the microbial communities, etc. At the end, future perspectives of research on biofilms in water supply pipes are discussed.

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Fabrication of a novel impedimetric sensor based on l-Cysteine/Cu(II) modified gold electrode for sensitive determination of ampyra

Cited by 33 publications

References 49 publications

Well-Orientation Strategy for Direct Immobilization of Antibodies: Development of the Immunosensor Using the Boronic Acid-Modified Magnetic Graphene Nanoribbons for Ultrasensitive Detection of Lymphoma Cancer Cells

Well-Orientation Strategy for Direct Immobilization of Antibodies: Development of the Immunosensor Using the Boronic Acid-Modified Magnetic Graphene Nanoribbons for Ultrasensitive Detection of Lymphoma Cancer Cells

Sensitive electrochemical determination of rifampicin using gold nanoparticles/poly-melamine nanocomposite

Biofilm Formation in Water Supply Pipes

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