A label-free DNA sensor based on impedance spectroscopy

Kafka, J. D.; Pänke, Oliver; Abendroth, Barbara; Lisdat, Fred

doi:10.1016/j.electacta.2008.01.031

Cited by 138 publications

(98 citation statements)

References 36 publications

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“…In nucleic acid detection, a common approach is to use the negatively charged redox couple potassium ferri-ferrocyanide for the measurement of R CT upon DNA recognition 10 . EIS has been used to detect DNA-DNA recognition at an electrode surface and numerous biosensors have been developed using this measurement approach in concert with a wide range of electrode types [11][12][13][14] . Specifically, for the detection of nucleic acids, studies have demonstrated the successful fabrication of microelectode devices using photolithographic methods and the development of nucleic acid sensors which use EIS as the measurement technique.…”

Section: Introductionmentioning

confidence: 99%

Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Piper

Schmueser

et al. 2017

Analyst

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Due to their electroanalytical advantages, microelectrodes are a very attractive technology for sensing and monitoring applications. One highly important application is measurement of DNA hybridisation to detect a wide range of clinically important phenomena, including single nucleotide polymorphisms (SNPs), mutations and drug resistance genes. The use of electrochemical impedance spectroscopy (EIS) for measurement of DNA hybridisation is well established for large electrodes but as yet remains relatively unexplored for microelectrodes due to difficulties associated with electrode functionalisation and impedimetric response interpretation. To shed light on this, microelectrodes were initially fabricated using photolithography and characterised electrochemically to ensure their responses matched established theory. Electrodes with different radii (50, 25, 15 and 5 µm) were then functionalised with a mixed film of 6-mercapto-1-hexanol and a thiolated single stranded ssDNA capture probe for a specific gene from the antibiotic resistant bacterium MRSA. The complementary oligonucleotide target from the mecA MRSA gene was hybridised with the surface tethered ssDNA probe. The EIS response was evaluated as a function of electrode radius and it was found that charge-transfer (R CT ) was more significantly affected by hybridisation of the mecA gene than the non-linear resistance (R NL ) which is associated with the steady state current. The discrimination of mecA hybridisation improved as electrode radius reduced with the R CT component of the response becoming increasingly dominant for smaller radii. It was possible to utilise these findings to produce a real time measurement of oligonucleotide binding where changes in R CT were evident one minute after nanomolar target addition. These data provide a systematic account of the effect of microelectrode radius on the measurement of hybridisation, providing insight into critical aspects of sensor design and implementation for the measurement of clinically important DNA sequences. The findings open up the possibility of developing rapid, sensitive DNA based measurements using microelectrodes.

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Section: Introductionmentioning

confidence: 99%

Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Piper

Schmueser

et al. 2017

Analyst

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“…No degradation over 30 cycles of DNA hybridization/denaturation can be detected. The overall performance of the diamond nano-wire DNA sensor in comparison to published data available in the literature for Au, [43][44][45][46] and polycrystalline diamond, [47] using comparable DNA mere structures are shown in Fig. 5e [47]).…”

Section: Resultsmentioning

confidence: 99%

Diamond nano-wires, a new approach towards next generation electrochemical gene sensor platforms

Nebel

Yang²,

Uetsuka³

et al. 2009

Diamond and Related Materials

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A novel bio-sensing platform is introduced by combination of a) geometrically controlled DNA bonding using vertically aligned diamond nano-wires and b) the superior electrochemical sensing properties of diamond as transducer material. Ultra-hard vertically aligned diamond nano-wires are electrochemically modified to bond phenyl linker-molecules to their tips which provide mesospacing for DNA molecules on the transducer. The nano-wires are generated by reactive ion etching of metallically boron doped atomically smooth single crystalline CVD diamond. Surface properties are characterized by atomic force, scanning electron and scanning tunneling microcopy. Electro-and bio-chemical sensor properties are investigated using cyclic and differential pulse voltammetry as well as impedance spectroscopy with Fe(CN) 6 3-/4-as redox mediators which reveal sensitivities of 2 pM on 3 mm 2 sensor areas and superior DNA bonding stability over 30 hybridization/ denaturation cycles. The fabrication of "all diamond" ultra-micro-electrode (UME) arrays and multi-gene sensors are discussed taking into account the unique properties of diamond.

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“…The repulsion between the negative charge of [Fe(CN) 6 ] 3À/4À and the negative charge of the DNA phosphate backbone serves as the basis for these types of DNA sensors [8,9]. Electrochemical impedance spectroscopy (EIS) is a technique which allows us to monitor the extent of DNA hybridization due to its sensitivity to electron transfer, which occurs at the interface between the solid electrode and the liquid solution.…”

Section: Introductionmentioning

confidence: 99%

Single‐Walled Carbon Nanotubes Modified Gold Electrodes as an Impedimetric DNA Sensor

2010

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A gold surface modified with a self-assembled monolayer of 11-amino-1-undecanethiol (AUT) was used for the covalent immobilization of oxidized single-walled carbon nanotubes (SWNTs). The as-described SWNTs-modified substrate was subsequently used to attach single-stranded deoxyribonucleic acid (ssDNA) used as a substrate for DNA hybridization. Electrochemical impedance spectroscopy measurements were performed to follow the DNA hybridization process by using the redox couple [Fe(CN) 6 ] 3À/4À as a marker ion. Specifically, changes in charge transfer resistance obtained from the Nyquist plots were used as the sensing parameter of DNA hybridization. The substrate sensitivity towards changes in target DNA concentration, its selectivity toward different DNA sequences and its reusability are successfully demonstrated in this report.

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A label-free DNA sensor based on impedance spectroscopy

Cited by 138 publications

References 36 publications

Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Impedimetric measurement of DNA–DNA hybridisation using microelectrodes with different radii for detection of methicillin resistant Staphylococcus aureus (MRSA)

Diamond nano-wires, a new approach towards next generation electrochemical gene sensor platforms

Single‐Walled Carbon Nanotubes Modified Gold Electrodes as an Impedimetric DNA Sensor

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