A label-free electrochemical biosensor for the detection of alpha-thalassemia 1 (SEA deletion) carriers using screen-printed carbon electrodes

Thamwarokun, Areenuch; Kaset, Chollanot; Karuwan, Chanpen; Kamsong, Wichayaporn; Attapong, Jirapat; Chomean, Sirinart

doi:10.1016/j.biosx.2023.100385

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…Common bio‐sensing technologies include Surface Plasmon Resonance (SPR), voltammetric electrochemistry, and electrochemiluminescence techniques, among others 101–103 . These biological sensing technologies play important roles in medical diagnostics, life science research, environmental monitoring, and food safety.…”

Section: Bio‐sensing Technologymentioning

confidence: 99%

Intelligent point of care testing for medicine diagnosis

Gao,

2024

Interdisciplinary Medicine

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Intelligent point‐of‐care testing (iPOCT) is a rapidly advancing technology that combines molecular detection and bio‐sensing techniques to achieve instant and accurate analysis through advanced sensors and intelligent algorithms. Molecular detection technologies, such as nucleic acid isothermal amplification, biochip and microfluidics, enable rapid analysis of the chemical composition and concentration of samples, thereby determining their presence. Bio‐sensing technology utilizes the recognition properties of biomolecules in conjunction with sensors to detect specific biomolecules or biological processes. iPOCT technology holds broad prospects for application in the field of healthcare and is expected to enhance detection sensitivity, accuracy, and intelligence through further development. In this review, we delineate the developmental journey of iPOCT, followed by a discussion on the principles of molecular detection and bio‐sensing technology, as well as the design of iPOCT devices for health monitoring and disease diagnosis. Furthermore, we showcased illustrative examples of iPOCT applications in disease diagnosis, highlighting the integration of iPOCT technology with wearable devices and smartphones for attaining more precise and personalized diagnostic results.

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Section: Bio‐sensing Technologymentioning

confidence: 99%

Intelligent point of care testing for medicine diagnosis

Gao,

2024

Interdisciplinary Medicine

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“…These devices are highly desirable for their ability to provide sequence-specific information in a faster, simpler, and less costly manner than conventional assays such as PCR or RT-PCR. Genosensors find application across a diverse array of fields, including but not limited to the following: neurodegenerative diseases [19,20]; cancerous pathologies encompassing breast cancer [21][22][23], cervical cancer [24,25], gastric cancer [26,27] and colorectal cancer [28]; inherited diseases like coronary artery diseases [29,30], sickle cell anemia [31] and thalassemia [32,33]; and the detection of infectious pathogens (SARS-CoV-2 [34,35], Influenza A virus [36,37], Haemophilus influenza [38], and Mycobacterium tuberculosis [39,40]).…”

Section: Introductionmentioning

confidence: 99%

Designing a Simple Electrochemical Genosensor for the Detection of Urinary PCA3, a Prostate Cancer Biomarker

Mokni,

Tlili,

Khalij

et al. 2024

Micromachines

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This study investigates the feasibility of a simple electrochemical detection of Prostate Cancer Antigen 3 (PCA3) fragments extracted from patients’ urine, using a thiolated single-strand DNA probe immobilized on a gold surface without using a redox probe. To enhance the PCA3 recognition process, we conducted a comparative analysis of the hybridization location using two thiolated DNA probes: Probe 1 targets the first 40 bases, while Probe 2 targets the fragment from bases 47 to 86. Hybridization with PCA3 followed, using square wave voltammetry. The limit of detection of the designed genosenors were of the order of (2.2 ng/mL), and (1.6 ng/mL) for Probes 1 and 2, respectively, and the subsequent sensitivities were of the order of (0.09 ± 0.01) µA−1 · µg−1 · mL and (0.10 ± 0.01) µA−1 · µg−1 · mL. Specificity tests were then conducted with the sensor functionalized with Probe 2, as it presents better analytical performances. The electrochemical results indicate that the designed sensor can clearly discriminate a complementary target from a non-complementary one. A further modeling of the calibration curves with the Power Law/Hill model indicates that the dissociation constant increases by one order of magnitude, confirming the ability of the designed sensor to perfectly discriminate complementary targets from non-complementary ones.

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A label-free electrochemical biosensor for the detection of alpha-thalassemia 1 (SEA deletion) carriers using screen-printed carbon electrodes

Cited by 2 publications

References 19 publications

Intelligent point of care testing for medicine diagnosis

Intelligent point of care testing for medicine diagnosis

Designing a Simple Electrochemical Genosensor for the Detection of Urinary PCA3, a Prostate Cancer Biomarker

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