A label-free electrochemical DNA biosensor based on thionine functionalized reduced graphene oxide

Ye, Yongkang; Xie, Jingqi; Ye, Yingwang; Cao, Xiaodong; Zheng, Haisong; Xu, Xuan; Zhang, Qiang

doi:10.1016/j.carbon.2017.12.060

Cited by 62 publications

(20 citation statements)

References 34 publications

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“…Several approaches for DNA analysis systems such as optical (fluorescent, surface plasmon resonance luminescent, colorimetric, etc.) [1][2][3][4][5], piezoelectric [6], or electrochemical [7][8][9] have been proposed. The electrochemical approach is promising because it can provide the above advantages whilst also offering the possibility of self-powered miniaturized devices relevant for point-of-care applications [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Influence of Graphene Oxide Concentration when Fabricating an Electrochemical Biosensor for DNA Detection

et al. 2019

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We have investigated the influence exerted by the concentration of graphene oxide (GO) dispersion as a modifier for screen printed carbon electrodes (SPCEs) on the fabrication of an electrochemical biosensor to detect DNA hybridization. A new pretreatment protocol for SPCEs, involving two successive steps in order to achieve a reproducible deposition of GO, is also proposed. Aqueous GO dispersions of different concentrations (0.05, 0.1, 0.15, and 0.2 mg/mL) were first drop-cast on the SPCE substrates and then electrochemically reduced. The electrochemical properties of the modified electrodes were investigated after each modification step by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), while physicochemical characterization was performed by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy. Finally, the sensing platform was obtained by the simple adsorption of the single-stranded DNA probe onto the electrochemically reduced GO (RGO)-modified SPCEs under optimized conditions. The hybridization was achieved by incubating the functionalized SPCEs with complementary DNA target and detected by measuring the change in the electrochemical response of [Fe(CN)6]3–/4– redox reporter in CV and EIS measurements induced by the release of the newly formed double-stranded DNA from the electrode surface. Our results showed that a higher GO concentration generated a more sensitive response towards DNA detection.

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Section: Introductionmentioning

confidence: 99%

Influence of Graphene Oxide Concentration when Fabricating an Electrochemical Biosensor for DNA Detection

et al. 2019

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“…So far there is a wide utilization of graphene and graphene-related nanomaterials in sensors and medical science [21,22,23,24] due to their small size, excellent biocompatibility, excellent conductivity, ease to be functionalized, high specific surface area, and mechanical strength [25,26,27]. Carboxyl graphene (CG) as a low-dimensional carrier for the growth of gold nanoparticles (Au NPs) enhances the electronic properties of graphene because of their space constraints and synergistic interactions.…”

Section: Introductionmentioning

confidence: 99%

Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera

Tao

Shui

et al. 2019

Biosensors

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The electrochemical aptamer sensor has been designed for detecting tau381, a critical biomarker of Alzheimer′s disease in human serum. The aptasensor is obtained by immobilizing the aptamer on a carboxyl graphene/thionin/gold nanoparticle modified glassy-carbon electrode. As a probe and bridge molecule, thionin connected carboxyl graphene and gold nanoparticles, and gave the electrical signal. Under optimal conditions, the increment of differential pulse voltammetry signal increased linearly with the logarithm of tau381 concentration in the range from 1.0 pM to 100 pM, and limit of detection was 0.70 pM. The aptasensor reliability was evaluated by determining its selectivity, reproducibility, stability, detection limit, and recovery. Performance analysis of the tau381 aptasensor in 10 patients’ serum samples showed that the aptasensor could screen patients with and without Alzheimer′s disease. The proposed aptasensor has potential for use in clinically diagnosing Alzheimer′s disease in the early stage.

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“…TH, diğer fenotiyazin boyaları gibi, iletken bir polimer oluşturmak için elektrokimyasal olarak polimerleştirilebilir. Son zamanlarda poli(tiyonin) (PTH), sensör ve biyosensörlerde hem iletken polimer hem de redoks medyatörü olarak kullanılan bir polimerdir [22][23][24]. Grafen tabakaları yüksek yüzey alanı, yüksek mekanik direnç ve mükemmel iletkenliğe sahip ultra hassas sensörlerin ve biyosensörlerin hazırlanmasında kullanılır [25][26].…”

Section: Abstract-dopamine Nadh Ascorbic Acid Electrooxidationunclassified

Dopamin, NADH ve Askorbik Asitin Elektroyükseltgenmesine Elektrokimyasal Olarak İndirgenmiş Grafen Oksit, Politiyonin ve Altın Nanopartiküllerin Etkisi

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2021

Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi

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Bu çalışmada, yüzey baskılı karbon elektrotlar (SPCE) elektrokimyasal olarak indirgenmiş grafen oksit (ERGO) ile modifiye edilmiş (SPCE/ERGO), ardından elektropolimerizasyon tekniği ile politiyonin (PTH) filmi SPCE/ERGO'lar üzerinde oluşturulmuştur (SPCE/ERGO/PTH). PTH'nin SPCE/ERGO'lar üzerindeki elektropolimerizasyonu için optimum döngü sayısı belirlenmiştir. SPCE/ERGO/PTH elektrotları üzerinde elektrodepozisyon metodu ile altın nanopartiküller (AuNP) oluşturulmuş ve elde edilen SPCE/ERGO/PTH/AuNP elektrotlarının elektrokimyasal karakterizasyonları dönüşümlü voltametri (CV) ile yapılmıştır. Dopamin (DA), nikotinamid adenin dinükleotitin indirgenmiş formu (NADH) ve askorbik asitin (AA) elektroyükseltgenmesine ERGO, PTH ve AuNP'nin elektrokatalitik etkileri incelenmiş ve PTH'nin üç analit için de medyatör olarak rol oynadığı gözlenmiştir. ERGO, PTH ve AuNP'nin birlikte gösterdikleri sinerjik etki ile DA, NADH ve AA'nın anodik pik potansiyelleri negatif yöne kaymış ve anodik pik akımları artmıştır. DA, NADH ve AA'nın difüzyon katsayıları (D) sırasıyla 3.37×10 −8 , 7.79×10 −9 ve 1.51×10 −8 olarak, elektron transfer katsayıları (α) ise sırasıyla 0.327, 0.701 ve 0.373 olarak hesaplanmıştır. pH'nın DA, NADH ve AA'nın elektroyükseltgenmesine etkisi de incelenmiş ve pH arttıkça her üç analit için de anodik pik potansiyellerinin azaldığı gözlenmiştir.

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A label-free electrochemical DNA biosensor based on thionine functionalized reduced graphene oxide

Cited by 62 publications

References 34 publications

Influence of Graphene Oxide Concentration when Fabricating an Electrochemical Biosensor for DNA Detection

Influence of Graphene Oxide Concentration when Fabricating an Electrochemical Biosensor for DNA Detection

Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera

Dopamin, NADH ve Askorbik Asitin Elektroyükseltgenmesine Elektrokimyasal Olarak İndirgenmiş Grafen Oksit, Politiyonin ve Altın Nanopartiküllerin Etkisi

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