A label-free electrochemical immunosensor based on poly(thionine)–SDS nanocomposites for CA19-9 detection

Jiang, Zhouqian; Zhao, Chengfei; Lin, Lin; Weng, Shaohuang; Liu, Qicai; Lin, Xinhua

doi:10.1039/c5ay00576k

Cited by 21 publications

(10 citation statements)

References 23 publications

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“…The corresponding calibration equation is ∆I (µA) = 1.58 + 5.39 log C (U•mL −1 ), having the regression coefficient (R 2 ) of 0.9974 and the limit of detection of 0.82 U•mL −1 based on a signal-to-noise ratio of 3 (S/N = 3). To better further evaluate the electrochemical immunosensors, the performance of our Zn-Co-S@G nanocomposite electrochemical immunosensor is compared with that of other electrochemical immunosensors for detecting the biomarker CA19-9, according to previous reports, which was better or comparable to that of the previous reports, as shown in Table 1 [1,6,[26][27][28][29].…”

Section: Resultsmentioning

confidence: 81%

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Construction of a Label-Free Electrochemical Immunosensor Based on Zn-Co-S/Graphene Nanocomposites for Carbohydrate Antigen 19-9 Detection

Tian

et al. 2021

Nanomaterials

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Nanocomposites of the binary transition metal sulfide Zn-Co-S/graphene (Zn-Co-S@G) were synthesized through a one-step hydrothermal method. They may be useful in the construction of an electrochemical immunosensor for carbohydrate antigen 19-9 (CA19-9) detection. Zn-Co-S dot-like nanoparticles uniformly covered the surface of graphene to form an interconnected conductive network, ensuring strong interaction between transition metal sulfide and graphene, which can expose numerous electroactive sites leading to the improvement of the amplified electrochemical signal toward a direct reduction of H2O2. Thus, the construction of an electrochemical immunosensor using Zn-Co-S@G nanocomposites showed outstanding sensing properties for detecting CA19-9. The constructed electrochemical immunosensor exhibited a good linear relationship in the range of 6.3 U·mL−1–300 U·mL−1, with the limit of detection at 0.82 U·mL−1, which makes it a promising candidate for an electrochemical immunosensor.

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Section: Resultsmentioning

confidence: 81%

“…(b) The linear range of BSA/anti-CA19-9/GA/CHIT/Zn-Co-S@G/GCE in Figure 8a. Polythionine-Au 0.26 6.5-520 [6] Polythionine/sodium dodecyl sulphate 0.45 5-400 [26] AuPt nanocalliandras 0.03 0.05-50 [27] Carbon nanoonions/grphene oxide 0.12 0.3-100 [28] Carbon black/polyelectrolytes 0.07 0.01-40 [29] Zn-Co-S/graphene 0.82 6.3-300 This work…”

Section: Resultsmentioning

confidence: 98%

Construction of a Label-Free Electrochemical Immunosensor Based on Zn-Co-S/Graphene Nanocomposites for Carbohydrate Antigen 19-9 Detection

Tian

et al. 2021

Nanomaterials

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“…2 In the past few years, various sensing methods have been developed for HBsAg detection, such as radioimmunoassay, 3 enzyme-linked immunosorbent assay, 4 chemiluminescence 5 methods and so on. 8,9 Meanwhile, this technique has been widely applied in product safety, 10 environmental detection, 11 clinical medicine, 12,13 etc. It shows obviously the advantages of high sensitivity, low cost and a simple pretreatment procedure.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical immunosensor for ultrasensitive detection of HBsAg based on platinum nanoparticles loaded on natural montmorillonite

Tian

Jia

et al. 2015

Anal. Methods

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A sensitive and facile electrochemical immunosensor for ultrasensitive detection of hepatitis B surface antigen (HBsAg) was designed, which was based on platinum nanoparticle decorated amino silane functionalized montmorillonite (Pt-NH 2 -MMT). As it is a luminosilicate clay mineral, the advantages of montmorillonite (MMT), such as a large specific surface area, good adsorption/ion exchange capacity and innocuousness, endow Pt-NH 2 -MMT with better sensing performance. The platinum nanoparticles (Pt NPs) also play an important role due to their good biological compatibility, electron transport rate and catalytic activity. Pt NPs and the available amine group of second HBsAg-antibody could be bound strongly to each other. And the covalent bond between NH 2 -MMT and the carboxyl group of primary HBsAg-antibody could be formed by the activation of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/ N-hydroxysuccinimide. Under optimal conditions, the Pt-NH 2 -MMT showed high electrocatalytic activity toward hydrogen peroxide reduction. Under optimal conditions, the immunosensor showed a wide linear response to HBsAg ranging from 0.5 pg mL À1 to 20 ng mL À1 with a low detection limit of 2.0 Â 10 À4 ng mL À1 . The designed immunosensor displayed high sensitivity, stability and reproducibility for the analysis of HBsAg.

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“…37,38 Regulation of polythionine can improve the ability and application performance in electrochemical biosensors. [39][40][41][42] Anionic surfactant-doped polythionine with sensitive response was recently prepared and used for the fabrication of label-free electrochemical immunosensor. 39 The previous work reported that polythionine and gold nanocomposites can be effectively synthesized and applied to detect biomarkers with high isoelectric point.…”

mentioning

confidence: 99%

“…[39][40][41][42] Anionic surfactant-doped polythionine with sensitive response was recently prepared and used for the fabrication of label-free electrochemical immunosensor. 39 The previous work reported that polythionine and gold nanocomposites can be effectively synthesized and applied to detect biomarkers with high isoelectric point. 40 Although reported works illustrated the fact that specific electrochemical biosensors for some targets based on polythionine or its composites can be obtained, the construction of facile label-free electrochemical sensing interface for large-scale analysis of biomarkers for variable clinical samples is still not available.…”

mentioning

confidence: 99%

Simple and effective label-free electrochemical immunoassay for carbohydrate antigen 19-9 based on polythionine-Au composites as enhanced sensing signals for detecting different clinical samples

Huang

Jiang

Zhao

et al. 2017

IJN

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Carbohydrate antigen 19-9 (CA19-9) is an important biomarker for the early diagnosis and clinical monitoring of pancreatic cancer. Reliable, simple, and accurate methods for the detection of CA19-9 are still urgently needed. In this study, polythionine-Au composites (AuNPs@ PThi) were designed and prepared through one-pot reaction using HAuCl 4 as the co-oxidant and raw material in thionine solution containing FeCl 3 as the oxidant. AuNPs@PThi-immobilized glassy carbon electrode was used as a sensitive redox probe for electrochemical interface. AuNPs@PThi not only favored the amplification of electrochemical signals but also facilitated excellent environmental friendliness for bioassay. Maximizing the electrochemical properties of AuNPs@PThi, an effective label-free electrochemical immunoassay for the ultrasensitive and reliable detection of CA19-9 was developed. Under optimal conditions, the linear range of the proposed immunosensor was estimated to range from 6.5 to 520 U/mL, with a detection limit of 0.26 U/mL at a signal-to-noise ratio of 3. The prepared immunosensor for CA19-9 detection showed high sensitivity, stability, and reproducibility. Furthermore, the fabricated immunosensor based on AuNPs@PThi can effectively detect and distinguish clinical serum samples of pancreatic cancer and normal control with accuracy and convenience.

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A label-free electrochemical immunosensor based on poly(thionine)–SDS nanocomposites for CA19-9 detection

Abstract: In this paper, poly(thionine) doped sodium dodecyl sulphate nanocomposites (PThi–SDS NCs) were synthesised for the first time.

Cited by 21 publications

References 23 publications

Construction of a Label-Free Electrochemical Immunosensor Based on Zn-Co-S/Graphene Nanocomposites for Carbohydrate Antigen 19-9 Detection

Construction of a Label-Free Electrochemical Immunosensor Based on Zn-Co-S/Graphene Nanocomposites for Carbohydrate Antigen 19-9 Detection

An electrochemical immunosensor for ultrasensitive detection of HBsAg based on platinum nanoparticles loaded on natural montmorillonite

Simple and effective label-free electrochemical immunoassay for carbohydrate antigen 19-9 based on polythionine-Au composites as enhanced sensing signals for detecting different clinical samples

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