A Laboratory Assessment of Factors That Affect Bacterial Adhesion to Contact Lenses

Abstract

Adhesion of pathogenic microbes, particularly bacteria, to contact lenses is implicated in contact lens related microbial adverse events. Various in vitro conditions such as type of bacteria, the size of initial inoculum, contact lens material, nutritional content of media, and incubation period can influence bacterial adhesion to contact lenses and the current study investigated the effect of these conditions on bacterial adhesion to contact lenses. There was no significant difference in numbers of bacteria that adhered to hydrogel etafilcon A or silicone hydrogel senofilcon A contact lenses. Pseudomonas aeruginosa adhered in higher numbers compared to Staphylococcus aureus. Within a genera/species, adhesion of different bacterial strains did not differ appreciably. The size of initial inoculum, nutritional content of media, and incubation period played significant roles in bacterial adhesion to lenses. A set of in vitro assay conditions to help standardize adhesion between studies have been recommended.

Study of the Effectiveness of Multipurpose Solutions on the Bacterial Disinfection of Silicone Hydrogel Contact Lenses In Vitro

“…Both of these characteristics are possible reasons for the finding that P. aeruginosa has more adherence than S. aureus , and it is a predominant causative agent in contact lens–induced infectious keratitis. 26 , 27 …”

Fabrication of a Stable and Efficient Antibacterial Nanocoating of Zn‐CuO on Contact Lenses

“…Several factors influence bacterial adhesion to contact lenses, of which the most influential have been found in our experiments to be the inoculum size, incubation period and assay media. [22] An inoculumo f1 0 6 CFU mL À1 brings as ubstantial amount of bacteria in contactw ith the lenses, resulting in mediumt oh igh levels of bacterial adhesion. [22] The incubation time neededt om aximize adhesion is ap eriod of 24 h, [23] and av ery effective assay medium is the nutrient-rich TSB.…”

“…[22] An inoculumo f1 0 6 CFU mL À1 brings as ubstantial amount of bacteria in contactw ith the lenses, resulting in mediumt oh igh levels of bacterial adhesion. [22] The incubation time neededt om aximize adhesion is ap eriod of 24 h, [23] and av ery effective assay medium is the nutrient-rich TSB. [22] P. aeruginosa is ab iofilm-formingb acterium.…”

“…[22] The incubation time neededt om aximize adhesion is ap eriod of 24 h, [23] and av ery effective assay medium is the nutrient-rich TSB. [22] P. aeruginosa is ab iofilm-formingb acterium. The resultsp resented in the current work are of high importance since P. aeruginosa is the predominant causativea gent in contact lens-induced microbial keratitis.…”

Antimicrobial Role of Human Meibomian Lipids at the Ocular Surface

“…These strains have been used in earlier studies, [28][29][30] and characteristics of these bacterial strains are given in the Table. Bacterial cultures were always freshly streaked on LB (Luria-Bertani) plates and allowed to grow at 378C overnight before use. For long-term storage, glycerol cultures were maintained at À808C.…”