A laboratory method for selection of topical antimicrobial agents to treat infected burn wounds

Nathan, Paul; Law, Edward; Murphy, Daniel F.; MacMillan, Bruce G.

doi:10.1016/s0305-4179(78)80006-0

Cited by 113 publications

(55 citation statements)

References 8 publications

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“…Although studies by Nathan and colleagues (317) as well as others (193,207,367) have described an agar diffusion method for this purpose, its use remains experimental and it has not been widely adopted due to lack of reproducibility and standardization. Pathogenic burn wound isolates are spread over the surface of an agar plate with 6-mm wells cut into the agar that contain various concentrations of the topical antibiotic being tested (317). The size of the zone of inhibition is predictive of the agent's specific antibacterial activity.…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Burn Wound Infections

Church

Elsayed

Reid³

et al. 2006

Clin Microbiol Rev

1,620

1,345

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SUMMARYBurns are one of the most common and devastating forms of trauma. Patients with serious thermal injury require immediate specialized care in order to minimize morbidity and mortality. Significant thermal injuries induce a state of immunosuppression that predisposes burn patients to infectious complications. A current summary of the classifications of burn wound infections, including their diagnosis, treatment, and prevention, is given. Early excision of the eschar has substantially decreased the incidence of invasive burn wound infection and secondary sepsis, but most deaths in severely burn-injured patients are still due to burn wound sepsis or complications due to inhalation injury. Burn patients are also at risk for developing sepsis secondary to pneumonia, catheter-related infections, and suppurative thrombophlebitis. The introduction of silver-impregnated devices (e.g., central lines and Foley urinary catheters) may reduce the incidence of nosocomial infections due to prolonged placement of these devices. Improved outcomes for severely burned patients have been attributed to medical advances in fluid resuscitation, nutritional support, pulmonary and burn wound care, and infection control practices.

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Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Burn Wound Infections

Church

Elsayed

Reid³

et al. 2006

Clin Microbiol Rev

1,620

1,345

View full text Add to dashboard Cite

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“…Prior studies assessing topical agent antimicrobial resistance profiles have used agar well diffusion and broth microdilution methods with data indicating an increase in resistance of MDR pathogens to topical antimicrobials as compared to non-MDR pathogens [8,[22][23][24]. However, there are limited data comparing strain variability and systemic antimicrobial resistance to topical antimicrobial activity tested by various methods.…”

Section: Introductionmentioning

confidence: 99%

Activity of topical antimicrobial agents against multidrug-resistant bacteria recovered from burn patients

Glasser

Guymon

Mende

et al. 2010

Burns

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“…It has long been known that wound infection can delay wound healing and cause increased morbidity and associated health care costs (3), and this has led to the introduction of medicated dressings. Due to concerns over the increased incidence of microbial resistance particularly associated with nosocomial infections, antimicrobial-impregnated dressings tend to utilize broad-spectrum antiseptic agents and numerous methods have been described to assess their antimicrobial efficacy (13,24,30).…”

mentioning

confidence: 99%

Use of a Bioluminescent Pseudomonas aeruginosa Strain within an In Vitro Microbiological System, as a Model of Wound Infection, To Assess the Antimicrobial Efficacy of Wound Dressings by Monitoring Light Production

Thorn

Nelson

Greenman

2007

Antimicrob Agents Chemother

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A bioluminescent Pseudomonas aeruginosa was incorporated into an in vitro static diffusion method to determine whether light output could be used as a measure of wound dressing efficacy. A significant linear correlation was observed between viable counts and bioluminescence during exponential growth in planktonic culture (r 2 ‫؍‬ 0.969). Exponential-phase cells were used to inoculate cellulose discs for integration into an in vitro wound model that incorporated a reservoir of serum. A significant linear correlation was found between bioluminescence (photon counts monitored by a low-light camera) and viable counts in this growth environment (r 2 ‫؍‬ 0.982). Three antimicrobial wound dressings were applied to the surface of freshly prepared sample discs within the wound model, and the kill kinetics were codetermined by photon and viable counts. Quantifiable kill rates gave the same order of efficacy for the three wound dressings using both types of measurement, and a significant linear correlation was shown between photon and viable counts (r 2 ‫؍‬ 0.873) within this killing environment. Under all defined conditions, a significant linear correlation between bioluminescence and viable counts was shown but the actual slope of the correlation was different, depending on the physicochemical environment of the cells. Hence, significantly more light per cell (P < 0.0001) was produced when cells in discs were exposed to a killing environment compared to a growing environment. As long as defined conditions are employed, the resulting linear correlation enables the state of the system to be continually monitored without disturbance, allowing more immediate and accurate calculations of kill rates without the need for viable counting.

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A laboratory method for selection of topical antimicrobial agents to treat infected burn wounds

Cited by 113 publications

References 8 publications

Burn Wound Infections

Burn Wound Infections

Activity of topical antimicrobial agents against multidrug-resistant bacteria recovered from burn patients

Use of a Bioluminescent Pseudomonas aeruginosa Strain within an In Vitro Microbiological System, as a Model of Wound Infection, To Assess the Antimicrobial Efficacy of Wound Dressings by Monitoring Light Production

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