Activity of topical antimicrobial agents against multidrug-resistant bacteria recovered from burn patients

Glasser, Jessie S.; Guymon, Charles H; Mende, Katrin; Wolf, Steven E.; Hospenthal, Duane R.; Murray, Clinton K.

doi:10.1016/j.burns.2010.05.013

Cited by 81 publications

(59 citation statements)

References 62 publications

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“…It has been reported in the literatures that the MIC of mafenide HCl against these bacteria is above 1,024 lg/ml [38], which is similar to the MIC of eluted mafenide from Lap/Maf samples ( Table 4). The area of inhibited zone showed that Lap/Maf gel extract generated comparable inhibitory zone to mafenide solution as control, which were in accordance with those reported in other studies [38,39]. The negative control (Laponite without mafenide) did not demonstrate any bactericidal effect, demonstrating that the materials alone without antibiotic do not have any antimicrobial properties.…”

Section: Anti-microbial Efficacysupporting

confidence: 62%

Antibiotic eluting clay mineral (Laponite®) for wound healing application: an in vitro study

Ghadiri

Chrzanowski

Rohanizadeh

2014

J Mater Sci: Mater Med

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Different materials in form of sponge, hydrogel and film have been developed and formulated for treating and dressing burn wounds. In this study, the potential of Laponite, a gel forming clay, in combination with an antimicrobial agent (mafenide), as a wound dressing material was tested in vitro. Laponite/mafenide (Lap/Maf) hydrogel was formulated in three different ratios of Lap/Maf 1:1, 1:2, 1:3. Laponite/mafenide/alginate (Lap/Maf/Alg) film was also formulated by combining Lap/Maf gel (1:1) with alginate. Intercalation rate of mafenide into the layers of Laponite nanoparticles and physico-chemical properties, including wound dressing characteristics of materials were studied using various analytical methods. Furthermore, the degradation of materials and the release profile of mafenide were investigated in simulated wound exudates fluid and antibacterial effectiveness of the eluted mafenide was tested on a range of bacterial species. The cytotoxicity of materials was also evaluated in skin fibroblast culture. The results showed that mafenide molecules were intercalated between the nano-sized layers of Laponite. The eluted mafenide showed active antibacterial effects against all three tested bacteria. All intercalated mafenide released from Lap/Maf 1:1 and 1:2 gel formulations and nearly 80% release from 1:3 formulation during test period. No significant difference was observed in release profile of mafenide between Lap/Maf/Alg film and Lap/Maf formulations. Wound dressing tests on Lap/Maf/Alg film showed it is a breathable dressing and has capacity to absorb wound exudates. The study showed that prepared Lap/Maf composite has the potential to be used as an antibiotic eluting gel or film for wound healing application. Additionally, Laponite has shown benefits in wound healing processes by releasing Mg(2+) ions and thereby reducing the cytotoxic effect of mafenide on fibroblast cells.

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Section: Anti-microbial Efficacysupporting

confidence: 62%

Antibiotic eluting clay mineral (Laponite®) for wound healing application: an in vitro study

Ghadiri

Chrzanowski

Rohanizadeh

2014

J Mater Sci: Mater Med

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“…These microorganisms have come into prominence as a cause of sepsis in many burn units. [11] Antibiotics should not be used for prophylaxis in burn, because it is well known that prophylactic antibiotic usage leads to colonization of resistant strains. [12] In cases where sepsis is strongly suspected, the initial antibiotic regimen to be delivered until the laboratory results are obtained should cover these organisms.…”

Section: Discussionmentioning

confidence: 99%

Clinical Infection in Burn Patients and the Consequences

Güldoğan

Kendirci²,

Tikici³

et al. 2017

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: Burn injuries facilitate invasive infections and sepsis not only by destroying the continuity of the protective skin barrier but also through systemic effects. The burn wound, blood, and urine samples are frequently cultured to determine the pathogen agent. The aim of this study was to analyze pathogen growth in patients' cultures confirmed as "infection positive" by the hospital Infection Control Committee and to assess the clinical implications of these growths.

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“…The same film then rapidly released NO when exposed to short pulses of visible light. In addition, when a PUX-NO film was placed on top of an agar plate containing the Griess reagent and incubated at 37 C for 16 h in the dark, no pink coloration (due to formation of azo dye) was observed. PUX-NO films thus exhibit no thermal release of NO at physiological temperatures.…”

Section: Discussionmentioning

confidence: 99%

“…The plates were then incubated as described above. The ''suspension assay'' was performed by diluting 100 lL of the E. coli working stock with 900 lL of TSB and incubating this culture at 37 C until an A 600 of 0.7 was reached. 50 lL of this solution was added to 100 mL of 0.8% (w/v) agar with 1% NaCl, which had been autoclaved and cooled to 47 C before the addition of the culture.…”

Section: Antimicrobial Effects Of Pux-no Filmsmentioning

confidence: 99%

Synthesis, characterization, and light‐controlled antibiotic application of a composite material derived from polyurethane and silica xerogel with embedded photoactive manganese nitrosyl

Heilman¹,

Halpenny²,

Mascharak³

2011

J Biomed Mater Res

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The synthesis of a light-sensitive polyurethane-based composite material (PUX-NO) is described. In its polyurethane medium, PUX-NO contains entrapped silica xerogel particles in which a photoactive manganese nitrosyl has been incorporated. Green flexible films of PUX-NO readily release nitric oxide (NO) only when exposed to low power (mW) visible light. Incorporation of the nitrosyl in the xerogel not only retains the nitrosyl (NO donor) within the composite material but also provides the right extent of hydration. Pre-swelled films of PUX-NO have water content close to 30 Wt % and such films can be stored for months under slightly moist condition without loss in NO-delivering capacity. The NO-releasing parameters of the film have been determined. The NO-releasing capacity of PUX-NO films can be conveniently altered by changing the amount of the nitrosyl as well as the thickness of the films. Patches of PUX-NO film have been successfully employed to reduce drastically bacterial loads of both gram-positive and gram-negative bacteria including methicillin-resistant Staphylococcus aureus and Acinetobacter baumannii under the total control of light. Effective control of infections by these bacterial pathogens via delivery of proper doses of NO only to the sites of infection appears feasible with PUX-NO films.

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Activity of topical antimicrobial agents against multidrug-resistant bacteria recovered from burn patients

Cited by 81 publications

References 62 publications

Antibiotic eluting clay mineral (Laponite®) for wound healing application: an in vitro study

Antibiotic eluting clay mineral (Laponite®) for wound healing application: an in vitro study

Clinical Infection in Burn Patients and the Consequences

Synthesis, characterization, and light‐controlled antibiotic application of a composite material derived from polyurethane and silica xerogel with embedded photoactive manganese nitrosyl

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