A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation

Chi, Zhanyou; Pyle, Denver; Wen, Zhiyou; Frear, Craig; Chen, Shulin

doi:10.1016/j.procbio.2007.08.008

Cited by 333 publications

(194 citation statements)

References 27 publications

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“…The closest species genetically to those studied in this work are Rhodotorula and Rhodosporidium (97%), both characterized as lipid and carotenoid producers (Saenge et al 2011, Freitas et al 2014. A more distant similarity (81%) was found with species of the Thraustochytriaceae family, characterized by high levels of DHA production (Chi et al 2007). The strains C22, C24, C30, C3 and C36 share the same cluster with Rhodotorula sp.…”

Section: Discussionmentioning

confidence: 52%

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Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Pino¹,

Socias²,

González³

2015

Rev. biol. mar. oceanogr.

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Resumen.-La creciente demanda de ácidos grasos poliinsaturados (AGPI) provocada por la escasez de pesca extractiva, ha impulsado la obtención de fuentes alternativas sustentables para abastecer su mercado. Los Thraus tochytridos y levaduras marinas (fungoides marinos), son potenciales productores de lípidos y carotenoides de importancia comercial. Desde una colección de 41 cepas de fungoides marinos aisladas en el Sistema de Corriente de Humboldt, se determinó la capacidad de producir DHA (ácido docosahexanoico), EPA (ácido eicosapentanoico) y CT (carotenoides totales) en cultivos líquidos comercial (MS) y alternativos (PDB, GAM, BAM y MCM). Los resultados indican que el medio MS y PDB, presentaron las mayores velocidades de crecimiento (0,02 h -1 ). No obstante, el medio MS presenta estos parámetros cinéticos a 16ºC y el PDB a 37°C. Trece de las cepas analizadas presentaron una alta capacidad para producir DHA (hasta 23% peso seco) y CT (hasta 18% peso seco), comparables a los niveles observados en Schizochytrium sp. KH105 y Rhodosporidium toruloides. Adicionalmente, todas las cepas ensayadas también produjeron pequeñas cantidades de EPA (hasta 0,3% peso seco). La microscopia electrónica revela que la cepa C36 es morfológicamente consistente con levaduras y su secuencia parcial del gen ribosomal 18s presenta un 97% de similitud con el género Rhodotorula, el que hasta ahora no ha sido reportado como productor de DHA y EPA. Finalmente, las cepas C36, C22 y C4 son interesantes objetos de estudio para escalar su producción y proyectar su uso comercial, como por ejemplo para suplementar en omega-3 y carotenoides alimentos para consumo humano, animales de corral o para estadios larvales de peces cultivados y pigmentar la carne del salmón, entre otros.Palabras clave: Fungoides marinos, DHA, EPA, carotenoides totales, Rhodotorula, parámetros cinéticos Abstract.-Declining fishing yields have pushed the search for sustainable alternative sources for polyunsatura ted fatty acids (PUFAs). Thraustochytrids and marine yeasts (marine fungoid protists) are potential commercial sources of lipids and carotenoids. It was determined the capacity of a collection of 41 strains of marine fungoid isol ated in the Humboldt Current System, to produce DHA (docosahexanoic acid), EPA (eicosapentanoic acid) and CT (total carotenoids) in commercial growing media (MS) and alternative growing mediums (PDB, GAM, BAM and MCM). The media MS and PDB exhibited the highest growth rate (0.02 h -1 ), at 16 and 37°C, respectively. Thirteen of the studied strains showed high capacity to produce DHA (up to 23% dry weight) and CT (up to 18% dry weight), comparable to levels observed in Schizochytrium sp. KH105 and Rhodosporidium toruloides. Additionally, all studied strains produce small amounts of EPA (up to 0.3% of dry weight). Scanning electron microscopy reveals that strain C36 is morphologically consistent with yeasts, while partial sequencing of the 18s ribosomal gene shows 97% similarity to the genus Rhodotorula, which has not been reported until n...

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Section: Discussionmentioning

confidence: 52%

“…The strains C36 and C22 for their high DHA concentrations, which were higher than what is described in the literature (Bajpai et al 1991, Burja et al 2006, Yamasaki et al 2006, Chi et al 2007. Rhodosporidium toruloides and Rhodotorula glutinis produce around 70 and 33.7% of total lipids in dry weight, respectively.…”

Section: Discussionmentioning

confidence: 73%

Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Pino¹,

Socias²,

González³

2015

Rev. biol. mar. oceanogr.

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“…Control of nitrogen source availability is a well-documented form of manipulation (Flynn et al 1992;Navarro-Angulo & Robledo 1999). In some instances, using microalgal heterotrophic potential has been shown to be of value (Xu et al 2006;Chi et al 2007); microalgae are useful within some fermentation systems because, being photosynthetic, they have unique biochemical pathways not present in non-carbon fixing organisms. Our understanding, and hence our ability, to model the commercial viability of such approaches is weak.…”

Section: Optimization Of Microalgal Growth and Production Of Specificmentioning

confidence: 99%

Placing microalgae on the biofuels priority list: a review of the technological challenges

Greenwell

Laurens

Shields

et al. 2009

J. R. Soc. Interface.

721

359

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Microalgae provide various potential advantages for biofuel production when compared with 'traditional' crops. Specifically, large-scale microalgal culture need not compete for arable land, while in theory their productivity is greater. In consequence, there has been resurgence in interest and a proliferation of algae fuel projects. However, while on a theoretical basis, microalgae may produce between 10-and 100-fold more oil per acre, such capacities have not been validated on a commercial scale. We critically review current designs of algal culture facilities, including photobioreactors and open ponds, with regards to photosynthetic productivity and associated biomass and oil production and include an analysis of alternative approaches using models, balancing space needs, productivity and biomass concentrations, together with nutrient requirements. In the light of the current interest in synthetic genomics and genetic modifications, we also evaluate the options for potential metabolic engineering of the lipid biosynthesis pathways of microalgae. We conclude that although significant literature exists on microalgal growth and biochemistry, significantly more work needs to be undertaken to understand and potentially manipulate algal lipid metabolism. Furthermore, with regards to chemical upgrading of algal lipids and biomass, we describe alternative fuel synthesis routes, and discuss and evaluate the application of catalysts traditionally used for plant oils. Simulations that incorporate financial elements, along with fluid dynamics and algae growth models, are likely to be increasingly useful for predicting reactor design efficiency and life cycle analysis to determine the viability of the various options for largescale culture. The greatest potential for cost reduction and increased yields most probably lies within closed or hybrid closed -open production systems.

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“…Response surface methodology is an approach that combines various statistical and mathematical techniques, and is useful for developing, improving and optimizing a process [11,15]. There are many applications of response surface methodology related to optimization of the composition of the growth media for microalgal growth [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Peyniraltı Suyu ve Mısır Islatma Şurubunun Heterotrofik Mikroalg Crypthecodinium cohnii ile Biyokütle ve Yağ Üretimi Amacıyla Biyolojik Olarak Değerlendirilmesi

İşleten-Hoşoğlu¹,

Elibol²

2017

Akademik Gıda

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Many different wastewater and by-products derived from industrial activities potentially support microalgal growth by providing a cost-effective and sustainable solutions. In this present study, it was aimed to biologically utilize cheese whey (CW) and corn steep liquor (CSL) for microalgal biomass and lipid production by using these wastes in culture media for heterotrophic microalga Crypthecodinium cohnii cultivation. To determine nutrient requirements for C. cohnii growing in a medium prepared with CSL and in CW, statistical screening tools were used. CSL significantly enhanced microalgal growth and it could be an alternative to yeast extract as the primary nutrient source. As for CW, it served as a good culture medium for C. cohnii with the supplement of some of nutrients and eliminated the need for fresh water. Thus, a new culture medium was developed by combining undiluted CW and CSL and optimized for the growth of C. cohnii. Lastly, in a scale-up attempt by using this new medium, microalgal production was performed in a 3 L stirred tank bioreactor. C. cohnii yielded relatively high biomass productivity (2.28 g/L.d) and lipid content (28.7% dry weight) in the optimized medium. Although C. cohnii was known for its ability to accumulate high amounts of docosahexaenoic acid (DHA), it transformed its fatty acid composition to an increased proportion of saturated and monounsaturated fatty acids (C16:0-C18:1) that comprise ~70% of total fatty acids (TFA) when it was cultivated in CW mainly supplemented with CSL. Thus, C. cohnii seemed to be more feasible for biodiesel production than any other purposes when it was cultivated in this new medium. Gıda 15(3) (2017) 233-241 Keywords 234üretimler gerçekleştirilmiştir. Optimizasyonu yapılan kültür ortamında kısmen yüksek biyokütle verimliliği (2.28 g/L.gün) ve yağ oranı (% 28.7 kuru ağırlık) sağlanmıştır. C. cohnii biyokütlede son derece yüksek oranlarda dokozahekzanoik asit (DHA) üreticisi bir tür olarak bilinmesine rağmen, CSL ile zenginleştirilmiş CW ortamında biyokütlesindeki yağ asidi kompozisyonunu değiştirerek, daha çok tekli doymuş ve tekli doymamış yağ asitlerince (C16:0-C18:1) zengin bir yağ profiline (toplam yağ asidi kompozisyonunun ~70%'i) sahip olduğu saptanmıştır. Buna göre, endüstriyel atıkların değerlendirildiği bu kültür ortamında yetiştirilen C. cohnii'nin biyodizel üretimi için uygun bir kaynak olabileceği sonucuna varılmıştır.

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A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation

Cited by 333 publications

References 27 publications

Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Placing microalgae on the biofuels priority list: a review of the technological challenges

Peyniraltı Suyu ve Mısır Islatma Şurubunun Heterotrofik Mikroalg Crypthecodinium cohnii ile Biyokütle ve Yağ Üretimi Amacıyla Biyolojik Olarak Değerlendirilmesi

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