A lateral flow immunoassay for rapid evaluation of vitellogenin levels in plasma and surface mucus of the copper redhorse (<i>Moxostoma hubbsi</i>)

Maltais, Domynick

doi:10.1897/06-481r.1

Cited by 10 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7), as previously shown by Maltais and Roy (2007) for copper redhorse VTG. Samples of plasma and surface mucus extracts from E2B-induced shorthead redhorse were also subjected to reducing SDS-PAGE and Western blot with the anti-carp VTG as primary antibody.…”

Section: Amino Acid Analysissupporting

confidence: 78%

“…In a previous study, we demonstrated that commercially available anti-carp VTG antibodies could be used to measure VTG in plasma and surface mucus of copper redhorse (Maltais and Roy 2007). In this study, we describe the purification and partial characterization of VTGs from both redhorse species and show antigenic cross-reactivity of the anti-carp VTG with plasma and mucus from the shorthead redhorse.…”

Section: Introductionmentioning

confidence: 71%

“…Sampling of blood and surface mucus Sampling of mucus and blood was performed as described in Maltais and Roy (2007). Samples of blood (500 ll before induction and as much as possible when the fish was sacrificed) and surface mucus were collected prior to the first E2B injection and 2-4 days after the last E2B injection.…”

Section: Source Of Fish and Induction With E2bmentioning

confidence: 99%

“…Western blots were performed as described in Maltais and Roy (2007). Briefly, proteins were electroblotted from the gel onto a polyvinylidene fluoride (PVDF) membrane (Immobilon-P, Millipore) using an XCell II TM Blot Module.…”

Section: Western Blotmentioning

confidence: 99%

“…In contrast, there can be a lack of crossreactivity even between closely related species (Campbell and Idler 1980;So et al 1985;Watts et al 2003). However, it has been demonstrated that antibodies against carp (Cyprinus carpio) VTG show good cross-reactivity with VTGs of several other cypriniformes (Tyler and Sumpter 1990a;Tyler et al 1996;Nilsen et al 2004) including the copper redhorse (Maltais and Roy 2007).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Purification and partial characterization of vitellogenin from shorthead redhorse (Moxostoma macrolepidotum) and copper redhorse (Moxostoma hubbsi) and detection in plasma and mucus with a heterologous antibody

Maltais

2008

Fish Physiol Biochem

View full text Add to dashboard Cite

Vitellogenin (VTG), the egg yolk precursor protein, was purified from plasma of estradiol-3-benzoate (E2B)-treated male shorthead redhorse (Moxostoma macrolepidotum) and immature copper redhorse (Moxostoma hubbsi) by a two-step chromatographic procedure without precipitation. Intact VTGs appeared as dimers with apparent molecular masses, determined by gel filtration, of approximately 425 kDa (copper redhorse) and approximately 450 kDa (shorthead redhorse). In native polyacrylamide gel electrophoresis (PAGE), dimeric redhorse VTGs appeared as a 520 kDa band. Both VTGs were reduced to a single monomer of approximately 150 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing and nonreducing conditions, indicating that monomers are not linked by disulfide bonds in the dimer form. The purified proteins were characterized as phospholipoglycoproteins. Isoelectric focusing of both VTGs revealed components with isoelectric points ranging from 5.3 to 6.0, suggesting charge heterogeneity. The amino acid composition of both VTGs contains a high proportion of nonpolar amino acids and was similar to those of other teleosts. An antibody developed against carp (Cyprinus carpio) VTG showed cross-reactivity with VTG from both redhorse species. Using this antibody, VTG was detected in plasma and surface mucus of E2B-treated redhorse. This is the most extensive report on purification and characterization of vitellogenin from catostomidid species.

show abstract

Section: Amino Acid Analysissupporting

confidence: 78%

Section: Introductionmentioning

confidence: 71%

Section: Source Of Fish and Induction With E2bmentioning

confidence: 99%

Section: Western Blotmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Purification and partial characterization of vitellogenin from shorthead redhorse (Moxostoma macrolepidotum) and copper redhorse (Moxostoma hubbsi) and detection in plasma and mucus with a heterologous antibody

Maltais

2008

Fish Physiol Biochem

View full text Add to dashboard Cite

show abstract

Purification and partial characterization of vitellogenin from spotted wolffish (Anarhichas minor) and development of an enzyme-linked immunosorbent assay for the determination of gender and sexual maturity

Maltais

Cyr

François

2013

Fish Physiol Biochem

View full text Add to dashboard Cite

Vitellogenin (VTG) from spotted wolffish, Anarhichas minor, a candidate species for cold-water marine aquaculture, was purified by MgCl₂/EDTA precipitation followed by a two-step chromatographic procedure. VTG had an apparent molecular mass of 470 kDa, as determined by gel filtration, and an amino acid composition similar to those of other teleosts. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of the purified VTG revealed a major band with a relative molecular weight of 166 kDa and some minor bands. Spotted wolffish VTG (sw-VTG) is relatively robust to in vitro degradation, as shown when samples of purified VTG and plasma from mature females subjected to various storage conditions or multiple freeze/thaw cycles were analyzed by Western blot. We developed an indirect competitive enzyme-linked immunosorbent assay (ELISA) using an antibody against Atlantic wolffish (Anarhichas lupus) VTG and purified sw-VTG. The ELISA had a detection limit of 6.7 ng/ml and a working range of 16.2-787.5 ng/ml, with intra- and inter-assay coefficients of variation ranging from 1.5 to 7.3 % and 7.1 to 14.3 %, respectively. The assay could distinguish males from immature females and discriminate maturing females at different stage of oocyte development. These results suggest that the sw-VTG ELISA would be useful in spotted wolffish aquaculture to determine sex and monitor female maturation.

show abstract

Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes)

Vázquez

Meijide

Cuña

et al. 2009

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

A lateral flow immunoassay for rapid evaluation of vitellogenin levels in plasma and surface mucus of the copper redhorse (Moxostoma hubbsi)

Cited by 10 publications

References 24 publications

Purification and partial characterization of vitellogenin from shorthead redhorse (Moxostoma macrolepidotum) and copper redhorse (Moxostoma hubbsi) and detection in plasma and mucus with a heterologous antibody

Purification and partial characterization of vitellogenin from shorthead redhorse (Moxostoma macrolepidotum) and copper redhorse (Moxostoma hubbsi) and detection in plasma and mucus with a heterologous antibody

Purification and partial characterization of vitellogenin from spotted wolffish (Anarhichas minor) and development of an enzyme-linked immunosorbent assay for the determination of gender and sexual maturity

Exposure to waterborne 4-tert-octylphenol induces vitellogenin synthesis and disrupts testis morphology in the South American freshwater fish Cichlasoma dimerus (Teleostei, Perciformes)

Contact Info

Product

Resources

About