A Leishmania donovani gene that confers accelerated recovery from stationary phase growth arrest

Hoyer, Cornelia; Zander, Dorothea; Fleischer, Sandra; Schilhabel, Markus B.; Kroener, Manfred; Platzer, Mathias; Clos, Joachim

doi:10.1016/j.ijpara.2004.02.006

Cited by 17 publications

(17 citation statements)

References 34 publications

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“…The add back gene in the 18S rRNA locus shows a 25-fold higher LdSGT RNA level compared with LdSGT +/+ . This is in keeping with previous results with the pIRmcs3+ integration vector (Hoyer et al 2004).…”

Section: Replacement Of Sgt In L Donovanisupporting

confidence: 93%

“…The polymerase chain reaction (PCR) product was then ligated between the XbaI and BglII sites of the integration vector pIRmcs3+ (Hoyer et al 2004). After construction, the plasmids were amplified in E. coli, purified by cesium chloride density gradient ultracentrifugation, and linearized with SwaI (Fig.…”

Section: Construction and Preparation Of Overexpression Systemsmentioning

confidence: 99%

“…8). The eGFP coding sequence was amplified with Acc65I and BamHI sites at the 5′ and 3′ ends and fused between the Acc65I and BglII sites of pIRmcs3+ (Hoyer et al 2004) to yield pIReGFP. A 3,506-bp SacI-SalI fragment from pcosTL (Kelly et al 1994) was cloned between the SacI and SalI sites of pUC19 (Yanisch-Perron et al 1985).…”

Section: Construction and Preparation Of Overexpression Systemsmentioning

confidence: 99%

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The co-chaperone SGT of Leishmania donovani is essential for the parasite's viability

Ommen

Chrobak

Clos

2010

Cell Stress and Chaperones

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Molecular chaperone proteins play a pivotal role in the protozoan parasite Leishmania donovani, controlling cell fate and ensuring intracellular survival. In higher eukaryotes, the so-called co-chaperone proteins are required for client protein recognition and proper function of chaperones, among them the small glutamine-rich tetratricopeptide repeat proteins (SGT) which interact with both HSP70 and HSP90 chaperones. An atypical SGT homolog is found in the L. donovani genome, encoding a protein lacking the C-terminal glutamine-rich region, normally typical for SGT family members. The gene is expressed constitutively during the life cycle and is essential for survival and/or growth of the parasites. LdSGT forms large, stable complexes that also include another putative cochaperone, HSC70 interacting protein (HIP). The gene product forms cytoplasmic clusters, matching the subcellular distribution of HIP and partly that of the major cytoplasmic chaperones, HSP70 and HSP90, reflecting a direct molecular interaction with both chaperones.

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Section: Replacement Of Sgt In L Donovanisupporting

confidence: 93%

Section: Construction and Preparation Of Overexpression Systemsmentioning

confidence: 99%

Section: Construction and Preparation Of Overexpression Systemsmentioning

confidence: 99%

See 1 more Smart Citation

The co-chaperone SGT of Leishmania donovani is essential for the parasite's viability

Ommen

Chrobak

Clos

2010

Cell Stress and Chaperones

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“…In another, more recent example, a protein which has a strong impact on the cell cycle of L. donovani and L. major was identified [48]. The gene, tentatively termed Leishmania donovani Growth Factor 1 (LdGF1), was selected under long-term in vitro cultivation.…”

Section: Examples For Functional Cloning In Leishmaniamentioning

confidence: 99%

“…Secondly, the necessary period of in vitro amplification of the selected sub-population following the functional selection will favor cells with fast in vitro growth rates. Under these conditions, a considerable selection within the survivor population can happen, favouring or eliminating previously selected cosmids [48]. This can be avoided, however, by early division of the survivor populations into clones, e.g.…”

Section: The Caveatsmentioning

confidence: 99%

Functional Cloning as a Means to Identify Leishmania Genes Involved in Drug Resistance

Clos¹,

Choudhury

2006

MRMC

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Resistance to anti-leishmanial drugs is a mounting problem in high-endemicity regions of South Asia and, potentially, in the context of HIV-Leishmania coinfections in Southern Europe. The molecular basis for clinical drug resistance is still largely unknown. It is important, however, to identify all relevant drug resistance markers for further drug development and for epidemiological surveys. An elegant and powerful method to identify such drug resistance markers without bias is functional cloning, using cosmid-based genomic DNA libraries. This review discusses the merits and caveats of this approach.

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Improvement of the green fluorescent protein reporter system in Leishmania spp. for the in vitro and in vivo screening of antileishmanial drugs

et al. 2012

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A Leishmania donovani gene that confers accelerated recovery from stationary phase growth arrest

Cited by 17 publications

References 34 publications

The co-chaperone SGT of Leishmania donovani is essential for the parasite's viability

The co-chaperone SGT of Leishmania donovani is essential for the parasite's viability

Functional Cloning as a Means to Identify Leishmania Genes Involved in Drug Resistance

Improvement of the green fluorescent protein reporter system in Leishmania spp. for the in vitro and in vivo screening of antileishmanial drugs

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