A lens-free single-shot fluorescent imaging system using CMOS image sensors with dielectric multi-layer filter

Imai, K.; Nishigaki, M.; Onozuka, Yutaka; Akimoto, Yusuke; Nagai, Miyu; Matsumoto, Satoru; Kousai, Shouhei

doi:10.1109/transducers.2017.7994007

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…If the absorption and emission peaks are not close, this effect is not significant [ 25 ]. However, for example, in the case of imaging cells containing GFP, the peaks are close to each other.…”

Section: Problems Of Conventional Emission Filters In Lens-free Immentioning

confidence: 99%

Highly sensitive lens-free fluorescence imaging device enabled by a complementary combination of interference and absorption filters

Sasagawa

Kimura

Haruta

et al. 2018

Biomed. Opt. Express

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We report a lens-free fluorescence imaging device using a composite filter composed of an interference filter and an absorption filter, each applied to one side of a fiber optic plate (FOP). The transmission of angled excitation light through the interference filter is absorbed by the absorption filter. The auto-fluorescence of the absorption filter is reduced by the reflection from the interference filter of normally incident excitation light. As a result, high-performance rejection of excitation light is achieved in a lens-free device. The FOP provides a flat, hard imaging device surface that does not degrade the spatial resolution. We demonstrate excitation rejection of approximately 108:1 at a wavelength of 450 nm in a fabricated lens-free device. The resolution of fluorescence imaging is approximately 12 µm. Time-lapse imaging of cells containing green fluorescent protein was performed in a 5-µm thin-film chamber. The small dimensions of the device allow observation of cell culturing in a CO2 incubator. We also demonstrate that the proposed lens-free filter is compatible with super-resolution bright-field imaging techniques. These features open a way to develop a high-performance, dual-mode, lens-free imaging device that is expected to be a powerful tool for many applications, such as imaging of labeled cells and point-of-care assay.

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Section: Problems Of Conventional Emission Filters In Lens-free Immentioning

confidence: 99%

Highly sensitive lens-free fluorescence imaging device enabled by a complementary combination of interference and absorption filters

Sasagawa

Kimura

Haruta

et al. 2018

Biomed. Opt. Express

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“…For instance, lensless microscopy [ 20 ] takes advantage of the large field-of-view (FOV) and small pixel size provided by large commercial cameras (CMOS or CCD). Various methods of lensless imaging can be found today, whether based on shadowing [ 21 , 22 , 23 ], fluorescence [ 24 , 25 , 26 ], holography [ 27 , 28 , 29 ], or 3D imaging [ 30 , 31 ]. The refinement of high-resolution (HR) techniques and the improvement of computational resources [ 32 , 33 , 34 ] have boosted the spatial resolution below the pixel size limitation.…”

Section: Introductionmentioning

confidence: 99%

Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy

Moreno

Canals

Moro

et al. 2021

Sensors

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Recent research into miniaturized illumination sources has prompted the development of alternative microscopy techniques. Although they are still being explored, emerging nano-light-emitting-diode (nano-LED) technologies show promise in approaching the optical resolution limit in a more feasible manner. This work presents the exploration of their capabilities with two different prototypes. In the first version, a resolution of less than 1 µm was shown thanks to a prototype based on an optically downscaled LED using an LED scanning transmission optical microscopy (STOM) technique. This research demonstrates how this technique can be used to improve STOM images by oversampling the acquisition. The second STOM-based microscope was fabricated with a 200 nm GaN LED. This demonstrates the possibilities for the miniaturization of on-chip-based microscopes.

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“…Nearinfrared filters and visible filters made of alternating SiO 2 and TiO 2 are deposited on the surface of a CIS in [124]. An emission filter made of a stack of 25 pairs of SiO 2 and Ti 3 O 5 is deposited on a CIS in [125]. A long-pass filter made of 20 layers of ZnS and Chapter 5.…”

Section: Discussionmentioning

confidence: 99%

Electrochemical and optical CMOS sensors design for biochemical detection

Jiang¹

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The contributions of the co-authors are as follows:• Prof Yu provided the initial ideal and edited the manuscript drafts.• Prof. Zhang and Prof. Feng revised the manuscript drafts.• I prepared the manuscript drafts, designed the ISFET pixel array with the pH-TVC readout circuit, worked in the full chip integration, built the testing system, and performed all the measurements, experiments, and data processing.

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A lens-free single-shot fluorescent imaging system using CMOS image sensors with dielectric multi-layer filter

Cited by 4 publications

References 13 publications

Highly sensitive lens-free fluorescence imaging device enabled by a complementary combination of interference and absorption filters

Highly sensitive lens-free fluorescence imaging device enabled by a complementary combination of interference and absorption filters

Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy

Electrochemical and optical CMOS sensors design for biochemical detection

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