A Leymus chinensis histidine-rich Ca2+-binding protein binds Ca2+/Zn2+ and suppresses abscisic acid signaling in Arabidopsis

Yang, Joon-Eon; Zhang, Ting; Mao, Huiping; Jin, Huiqing; Sun, Yawang; Qi, Zhi

doi:10.1016/j.jplph.2020.153209

Cited by 9 publications

(7 citation statements)

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“…This protein initially identified in many mammalian species, including rabbits, humans, mice, rats and monkeys [ 52 – 55 ], and served as the regulators of Ca ion uptake, storage and release [ 56 ]. However, recent study about the homolog in L. chinensis found this gene might had distinct Ca ion binding sites and could interact with a histone deacetylation protein to mediate the transient rises for Ca ion concentration in plant nuclei [ 57 ]. Additionally, overexpressing the nucleus-localized Ca ion binding histidine-rich calcium-binding protein could alter Ca ion homeostasis in Arabidopsis nucleus [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study reveals the genetic architecture for calcium accumulation in grains of hexaploid wheat (Triticum aestivum L.)

Shi

Zhou

et al. 2022

BMC Plant Biol

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Background Hexaploid wheat (Triticum aestivum L.) is a leading cereal crop worldwide. Understanding the mechanism of calcium (Ca) accumulation in wheat is important to reduce the risk of human micronutrient deficiencies. However, the mechanisms of Ca accumulation in wheat grain are only partly understood. Results Here, a genome-wide association study (GWAS) was performed to dissect the genetic basis of Ca accumulation in wheat grain using an association population consisting of 207 varieties, with phenotypic data from three locations. In total, 11 non-redundant genetic loci associated with Ca concentration were identified and they explained, on average, 9.61–26.93% of the phenotypic variation. Cultivars containing more superior alleles had increased grain Ca concentrations. Notably, four non-redundant loci were mutually verified by different statistical models in at least two environments, indicating their stability across different environments. Four putative candidate genes linked to Ca accumulation were revealed from the stable genetic loci. Among them, two genes, associated with the stable genetic loci on chromosomes 4A (AX-108912427) and 3B (AX-110922471), encode the subunits of V-type Proton ATPase (TraesCS4A02G428900 and TraesCS3B02G241000), which annotated as the typical generators of a proton gradient that might be involved in Ca homeostasis in wheat grain. Conclusion To identify genetic loci associated with Ca accumulation, we conducted GWAS on Ca concentrations and detected 11 genetic loci; whereas four genetic loci were stable across different environments. A genetic loci hot spot exists at the end of chromosome 4A and associated with the putative candidate gene TraesCS4A02G428900. The candidate gene TraesCS4A02G428900 encodes V-type proton ATPase subunit e and highly expressed in wheat grains, and it possibly involved in Ca accumulation. This study increases our understanding of the genetic architecture of Ca accumulation in wheat grains, which is potentially helpful for wheat Ca biofortification pyramid breeding.

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Section: Discussionmentioning

confidence: 99%

Genome-wide association study reveals the genetic architecture for calcium accumulation in grains of hexaploid wheat (Triticum aestivum L.)

Shi

Zhou

et al. 2022

BMC Plant Biol

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Section: Cloning Resistance Genes From Common Wheat Using Forward Gen...mentioning

confidence: 99%

“…It was recently found that HRC/His-S from Leymus chinensis (named LcHRC in the original article), which showed identical amino acid sequence to wheat HRC/His-S, bound calcium and zinc ion in vitro ( Yang et al., 2020 ). Arabidopsis thaliana seedlings overexpressing LcHRC showed sensitivity to abscisic acid (ABA) ( Yang et al., 2020 ). A protein that participates in heterochromatin silencing was identified as an LcHRC interactor through the screening of Arabidopsis yeast cDNA library ( Yang et al., 2020 ).…”

Section: Cloning Resistance Genes From Common Wheat Using Forward Gen...mentioning

confidence: 99%

“…Arabidopsis thaliana seedlings overexpressing LcHRC showed sensitivity to abscisic acid (ABA) ( Yang et al., 2020 ). A protein that participates in heterochromatin silencing was identified as an LcHRC interactor through the screening of Arabidopsis yeast cDNA library ( Yang et al., 2020 ). These results suggest a potential role of LcHRC in the regulation of genes involved in abiotic stress response.…”

Section: Cloning Resistance Genes From Common Wheat Using Forward Gen...mentioning

confidence: 99%

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Exploring and applying genes to enhance the resistance to Fusarium head blight in wheat

Liu²,

Zhao³

et al. 2022

Front. Plant Sci.

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Fusarium head blight (FHB) is a destructive disease in wheat worldwide. Fusarium graminearum species complex (FGSC) is the main causal pathogen causing severe damage to wheat with reduction in both grain yield and quality. Additionally, mycotoxins produced by the FHB pathogens are hazardous to the health of human and livestock. Large numbers of genes conferring FHB resistance to date have been characterized from wheat and its relatives, and some of them have been widely used in breeding and significantly improved the resistance to FHB in wheat. However, the disease spreads rapidly and has been severe due to the climate and cropping system changes in the last decade. It is an urgent necessity to explore and apply more genes related to FHB resistant for wheat breeding. In this review, we summarized the genes with FHB resistance and mycotoxin detoxication identified from common wheat and its relatives by using forward- and reverse-genetic approaches, and introduced the effects of such genes and the genes with FHB resistant from other plant species, and host-induced gene silencing (HIGS) in enhancing the resistance to FHB in wheat. We also outlined the molecular rationale of the resistance and the application of the cloned genes for FHB control. Finally, we discussed the future challenges and opportunities in this field.

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“…Mixed breeding has produced a few sheepgrass varieties and molecular module breeding has not been applied in sheepgrass (Peng et al, 2011; Gao, 2021). Sheepgrass was transformed successfully with Agrobacterium ‐mediated transformation and bombardment system before (Liu et al, 2004; Shu et al, 2005; Wang et al, 2009; Di et al, 2021), while the lack of genetic manipulation system, such as clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR‐associated nuclease 9 (Cas9) (Shan et al, 2013; Wang et al, 2014), was still one of the factors holding back sheepgrass breeding (Zhao et al, 2019; Yang et al, 2020; Wei and Gao, 2022). Moreover, many Gramineae are recalcitrant to Agrobacterium ‐mediated transformation.…”

Section: Introductionmentioning

confidence: 99%

Efficient CRISPR/Cas9‐mediated genome editing in sheepgrass (Leymus chinensis)

Lin,

Chen,

Tang

et al. 2023

JIPB

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The lack of genome editing platforms has hampered efforts to study and improve forage crops that can be grown on lands not suited to other crops. Here, we established efficient Agrobacterium‐mediated CRISPR/Cas9 genome editing in a perennial, stress‐tolerant forage grass, sheepgrass (Leymus chinensis). By screening for active single‐guide RNAs (sgRNAs), accessions that regenerate well, suitable Agrobacterium strains, and optimal culture media, and co‐expressing the morphogenic factor TaWOX5, we achieved 11% transformation and 5.83% editing efficiency in sheepgrass. Knocking out Teosinte Branched1 (TB1) significantly increased tiller number and biomass. This study opens avenues for studying gene function and breeding in sheepgrass.This article is protected by copyright. All rights reserved.

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A Leymus chinensis histidine-rich Ca2+-binding protein binds Ca2+/Zn2+ and suppresses abscisic acid signaling in Arabidopsis

Cited by 9 publications

References 53 publications

Genome-wide association study reveals the genetic architecture for calcium accumulation in grains of hexaploid wheat (Triticum aestivum L.)

Genome-wide association study reveals the genetic architecture for calcium accumulation in grains of hexaploid wheat (Triticum aestivum L.)

Exploring and applying genes to enhance the resistance to Fusarium head blight in wheat

Efficient CRISPR/Cas9‐mediated genome editing in sheepgrass (Leymus chinensis)

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