A Light and Electron Microscopic Anterograde WGA-HRP Tracing Study on the Sensory Innervation of Junctional and Sulcular Epithelium in the Rat Molar

Kondo, Teruyoshi; Ayasaka, Norio; Nagata, Emi; Takano, Teruo

doi:10.1177/00220345920710011001

Cited by 25 publications

(13 citation statements)

References 31 publications

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“…These findings suggest that the PIE, vasculature, nerves, and leukocytes are targets of substance P in periimplant mucosal tissue. Kondo et al (1992) have provided evidence that trigeminal sensory fibers innervate the JE to a much greater degree than the oral sulcular or oral epithelium. Our immunoelectron-microscopic study has demonstrated that substance P, which is a neurotransmitter contained within C-fibers and occasionally within small Aδ fibers (Léránth et al 1984), is located in naked intraepithelial nerve endings adjacent to JE cells (Tanaka et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Distribution of substance P and neurokinin-1 receptors in the peri-implant epithelium around titanium dental implants in rats

et al. 2008

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We examined the distribution of substance P and neurokinin-1 (NK1) receptors and substance-P-containing nerve fibers in the peri-implant mucosa around titanium dental implants in rats. Immunohistochemistry and immunocytochemistry revealed that substance-P-immunoreactive nerve fibers abundantly innervated the peri-implant epithelium (PIE) compared with other epithelia of the peri-implant mucosa. NK1 receptor mRNA and protein expression in the peri-implant mucosa were confirmed by reverse transcription with the polymerase chain reaction and immunoblotting. Immunoelectron microscopy revealed that NK1 receptor immunoreactivity was preferentially localized in peri-implant epithelial cells. NK1-receptor-positive products were found on the plasma membrane and in vesicles and granules in PIE cells. Neutrophils and intraepithelial nerve axons in the PIE were positive for the NK1 receptor. NK1 receptor immunoreactivity was also detected in endothelial cells, fibroblasts, and nerve fibers in the connective tissue beneath the PIE. These findings suggest that peri-implant tissue receives sensory information through regenerated nerves expressing substance P and the NK1 receptor. In the peri-implant mucosa, the substance P/NK1 receptor system may play a role in pain transmission, the endocytosis of neutrophils, the extravasation of crevicular fluid, and the migration of macrophages and neutrophils in response to neurogenic inflammation, as in healthy gingiva.

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Section: Discussionmentioning

confidence: 99%

Distribution of substance P and neurokinin-1 receptors in the peri-implant epithelium around titanium dental implants in rats

et al. 2008

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“…These intraepithelial terminal regions of the axon have penetrated the basement membrane and lost their myelin sheath and are prone to varicosity formation. Strings of varicosities have been reported to occur in these intraepithelial terminal areas of the TG axons in vivo (Kondo et al, 1992, Ibuki et al, 1996). …”

Section: Discussionmentioning

confidence: 99%

α-Herpesvirus glycoprotein D interaction with sensory neurons triggers formation of varicosities that serve as virus exit sites

Regge¹,

Nauwynck²,

Geenen³

et al. 2006

The Journal of Cell Biology

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α-Herpesviruses constitute closely related neurotropic viruses, including herpes simplex virus in man and pseudorabies virus (PRV) in pigs. Peripheral sensory neurons, such as trigeminal ganglion (TG) neurons, are predominant target cells for virus spread and lifelong latent infections. We report that in vitro infection of swine TG neurons with the homologous swine α-herpesvirus PRV results in the appearance of numerous synaptophysin-positive synaptic boutons (varicosities) along the axons. Nonneuronal cells that were juxtaposed to these varicosities became preferentially infected with PRV, suggesting that varicosities serve as axonal exit sites for the virus. Viral envelope glycoprotein D (gD) was found to be necessary and sufficient for the induction of varicosities. Inhibition of Cdc42 Rho GTPase and p38 mitogen-activated protein kinase signaling pathways strongly suppressed gD-induced varicosity formation. These data represent a novel aspect of the cell biology of α-herpesvirus infections of sensory neurons, demonstrating that virus attachment/entry is associated with signaling events and neuronal changes that may prepare efficient egress of progeny virus.

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“…Interestingly, our laboratory (Kondo et al 1992, 1995; Tanaka et al 1996) reported that substance P (SP)‐containing nerve terminals exist adjacent to the JE cells. Furthermore, we (Kido et al 1999) found neurokinin 1 receptors (NK1‐R), known to have the highest affinity for SP (Regoli et al 1988), on JE cells but not on OE or OSE cells.…”

Section: Discussionmentioning

confidence: 99%

Difference in penetration of horseradish peroxidase tracer as a foreign substance into the peri‐implant or junctional epithelium of rat gingivae

Ikeda

Shiraiwa

Yamaza

et al. 2002

Clinical Oral Implants Res

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Horseradish peroxidase (HRP) tracer was applied to the gingival sulcus of implants or natural teeth at 5, 25, or 50 mg/ml to investigate the sealing capacities of the peri-implant epithelium (PIE) and junctional epithelium (JE); the extent of HRP penetration was observed under electron microscopy. A Ti-6Al-4V implant was inserted either immediately (immediate implantation) or 2 weeks (delayed implantation) after extraction of the maxillary left first molar of rats. The JE of the right molar was used as a control. Although the whole PIE of undecalcified sections appeared to be attached to the implant surface, electron microscopically, the internal basement lamina (IBL) and hemidesmosomes were deficient in the coronal-middle region of the PIE. There were extensive extracellular deposits of HRP in the intercellular spaces between PIE cells, and HRP was blocked to some extent by the lamina lucida and lamina densa of the external basal lamina and basal cell junction. HRP was detected in the connective tissue under the PIE, but was not found in the connective tissue under the JE. Intracellularly, HRP was found in the vesicles and granules of PIE cells and JE cells. These were fewer in number in PIE cells than in JE cells. There were no differences between the findings for immediate and delayed implantation. The results indicate that a deficiency in the IBL permitted penetration of HRP from the gingival sulcus into the connective tissue under the PIE, and suggest that the endocytotic capacity of PIE cells is inferior to that of JE cells.

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A Light and Electron Microscopic Anterograde WGA-HRP Tracing Study on the Sensory Innervation of Junctional and Sulcular Epithelium in the Rat Molar

Cited by 25 publications

References 31 publications

Distribution of substance P and neurokinin-1 receptors in the peri-implant epithelium around titanium dental implants in rats

Distribution of substance P and neurokinin-1 receptors in the peri-implant epithelium around titanium dental implants in rats

α-Herpesvirus glycoprotein D interaction with sensory neurons triggers formation of varicosities that serve as virus exit sites

Difference in penetration of horseradish peroxidase tracer as a foreign substance into the peri‐implant or junctional epithelium of rat gingivae

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