A Linkage Map of the Tomato Spotted Wilt Virus Resistance Gene Sw-5 Using Near Isogenic Lines and an Interspecific Cross

Stevens, Mikel R.; Heiny, Dana Kelly; Rhoads, David B.; Griffiths, Phillip D.; Scott, John F.

doi:10.17660/actahortic.1996.431.33

Cited by 10 publications

(8 citation statements)

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“…Sw-5 does not produce 100% resistance (98.7% penetrance, reviewed by Stevens et al 1996) and is semidominant (heterozygotes are less resistant than homozygotes, reviewed by Brommonschenkel et al 2000). It will be difficult or impossible to detect the resistance gene by screening for resistance in some cases.…”

Section: Resultsmentioning

confidence: 99%

The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

Garland¹,

Sharman²,

Persley³

et al. 2005

Aust. J. Agric. Res.

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Abstract. Sw-5 is an important disease resistance gene of tomato, providing broad resistance to Tomato spotted wilt virus (TSWV). A cleaved amplified polymorphic sequence (CAPS) marker, closely linked to the gene, has been reported. Although the Sw-5 locus has been characterised, a gene-specific marker has not been developed. This paper presents a PCR-based marker-system that consists of the co-amplification of a dominant marker representing the Sw-5 gene sequence, and the modified CAPS marker as a positive control and indicator of genotype.Additional keyword: rapid DNA extraction.

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Section: Resultsmentioning

confidence: 99%

The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

Garland¹,

Sharman²,

Persley³

et al. 2005

Aust. J. Agric. Res.

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“…The tospovirus resistance protein A of the gene Sw5-a is complement to the sequence of the Sw-5 locus located at AY007366 from 20824 to 24561 base and the tospovirus resistance protein B of the gene Sw5-b is complement to the sequence of the Sw-5 locus located at AY007366 from 29513 to 33253 base (Folkertsma et al1999, GenBank Accession AY007366). Garland et al (2005) reported a dominant PCR-based marker representing the Sw-5 gene sequence co-segregated with the RFLP marker CT 220 (Stevens et al 1995(Stevens et al , 1996 in 50 individuals of an F 2 mapping population.…”

Section: Introductionmentioning

confidence: 99%

“…Stevens et al (1995) mapped Sw-5 between restriction fragment length polymorphism (RFLP) markers CT71 and CT220 near a telomeric region of chromosome 9 and also reported one random amplified polymorphic DNA (RAPD) marker 421R likely within 0.5 cM of Sw-5. Using a segregating L. esculentum backcross population derived from near isogenic lines (NILs), Stevens et al (1996) further mapped the RAPD marker 421R linked to Sw-5 within a distance of 1 cM and the RFLP marker CT220 about 2.4 cM from Sw-5. Chague et al (1996) reported four RAPD markers linked to Sw-5 within a distance of 10.5 cM on chromosome 9 and one sequence characterized amplified region (SCAR) marker developed from the four RAPD makers.…”

Section: Introductionmentioning

confidence: 99%

Identification of molecular markers for Sw-5 gene of tomato spotted wilt virus resistance

Shi¹,

Vierling²

2011

AJBMS

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Tomato spotted wilt virus (TSWV) is one of the most destructive viral diseases that threaten worldwide tomato (Solanuum lycopersicum L. syn Lycopersicon esculentum Mill.) production. Use of host resistance appears to be the best way to control the disease. Marker assisted selection (MAS) has become very important and useful in the selection of TSWV resistance genes. The objective of this research was to identify Sw-5 gene-specific PCR-based molecular markers and gene-derived single nucleotide polymorphism (SNP) markers for MAS in tomato breeding. A gene-specific PCR-based marker and two gene-derived SNP markers specific for TSWV resistance Sw5-b allele were identified and validated in different tomato germplasm. It is expected that the present findings will be helpful in advancing the MAS for TSWV resistance in tomato.

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“…Several molecular markers associated to virus-resistant genes have been identified, although their use has not been frequent when they are not closely associated to the gene(s) with an interest allele (s) (Yu et al, 1993;Webb et al, 1995;Silva et al, 2003;Santos, 2004;Ribeiro et al, 2006). The distance between the Sw-421 marker and the Sw-5 allele is smaller than 1.0 cM (Stevens et al, 1996), and this allows the selection of the Sw-5 allele with a good safety margin.…”

Section: S H R H R S R H R S H R H R H H S S S S S S S S S S S S Smentioning

confidence: 99%

“…Resistance in the cultivar Stevens is controlled by one gene, denominated Sw-5 with dominant allelic interaction (Stevens et al, 1992;Juliatti & Maluf, 1995), whereas for the cultivar Rey de los Tempranos resistance is controlled by at least 1 to 3 genes with semi-dominant allelic interaction (Juliatti & Maluf, 1995). Stevens et al (1995) developed the UBC 421 marker from a RAPD (Random amplified polymorphic DNA) decamer primer (ACG GCC CAC C), which shows a 940 bp band (421R band) in resistant genotypes and a 900 bp band (421S band) in susceptible materials at a distance smaller than 1.0 cM from the Sw-5 gene, with band polymorphism of the co-dominance type; that is, two bands at the allegedly heterozygote genotypes (Stevens et al, 1996). The 'UBC 421' marker was converted in another co-dominant SCAR marker type (20 pb primers 'Sw-421-1 and Sw-421-2'), but its efficiency is still to be proved in advanced segregating populations of tomato plants.…”

Section: Introductionmentioning

confidence: 99%

Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies

Nascimento

Maluf²,

Figueira

et al. 2009

Sci. agric. (Piracicaba, Braz.)

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The SCAR (Sequence Characterized Amplified Region) 'Sw-421' molecular marker is located at 1.0 cM from the Sw-5 allele, originated from Lycopersicon peruvianum (L.), which confers resistance to the tomato spotted wilt virus (TSWV). However, it had not been tested yet in advanced tomato populations. The goal of this study was to distinguish resistant homozygotes (Sw-5/Sw-5) and heterozygotes (Sw-5/Sw-5 + ) from susceptible (Sw-5 + /Sw-5 + ) plants in crossing populations with the Stevens cultivar and advanced backcrossing populations by using 'Sw421' SCAR marker. The amplification of 940 bp and 900 bp bands characterized the resistant homozygotes and susceptible controls, respectively. A two band pattern (900 bp and 940 bp) was observed in heterozygote genotypes (Sw-5/Sw-5 + ), which confirmed the co-dominant inheritance mechanism of the marker. Fifty seven plants from the isogenic progenies were characterized based on bands pattern: 18 plants (31.6%) were identified as resistant homozygotes, 8 plants (14.0%) as resistant heterozygotes and 31 plants (54.4%) were characterized as susceptible. The SCAR 'Sw-421' marker is an important tool for selection and pyramid resistance alleles, mainly when other resistance sources to the TSWV are available, such as the Rey de los Tempranos source.

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A Linkage Map of the Tomato Spotted Wilt Virus Resistance Gene Sw-5 Using Near Isogenic Lines and an Interspecific Cross

Cited by 10 publications

References 0 publications

The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

Identification of molecular markers for Sw-5 gene of tomato spotted wilt virus resistance

Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies

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