A lipoprotein characterizing obstructive jaundice. I. Method for quantitative separation and identification of lipoproteins in jaundiced subjects

Seidel, D.; Alaupovic, P.; Furman, Robert H.

doi:10.1172/jci106085

Cited by 324 publications

(130 citation statements)

References 34 publications

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“…The abnormal fraction which appeared between the chylomicron and LDL fractions in lipoprotein electrophoresis might be suspected of being lipoprotein X (Lp-X) [47], which has been reported in patients with cholestatic liver diseases [11,33,44], patients with a deficiency of lecithin/cholesterol acyltransferase (LCAT) [16,36,48] and during intravenous infusion of fat emulsions [2,50,51]. Lp-X is a bilayer vesicle of phospholipids (51-66%) and free cholesterol (22-30%) and contains a small amount of proteins essentially including albumin and apoCs but also including apoE and apoAI [2,16,33,35,36,39,44,48].…”

Section: Discussionmentioning

confidence: 99%

APA Hamster Model for Diabetic Atherosclerosis. 2. Analysis of Lipids and Lipoproteins.

et al. 2000

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Syrian hamsters of the APA strain (APA hamsters) have recently been shown to have atheromatous lesions in the aortic arches under diabetic condition induced by a single injection of streptozotocin (SZ). In that model, fatty streaks, which are the initial lesions of atherogenesis, develop by 6 weeks after the injection (WAI). In this study, we evaluated plasma lipid concentrations and lipoprotein profiles in diabetic APA hamsters at 6 WAI to reveal the early stage of atherogenesis clinicopathologically. As a result, by biochemical analysis, hyperglycemic APA hamsters showed signs of hypercholesterolemia and hypertriglyceridemia. Low-density lipoprotein (LDL) cholesterol significantly increased, but high-density lipoprotein (HDL) cholesterol significantly decreased. Agarose gel electrophoresis showed an obvious increase in the fractions of chylomicron, LDL and abnormal lipoprotein. Plasma LDL in diabetic animals was in a state more susceptible to oxidization. In addition, a significant increase in glycated LDL was also found in the diabetic animals by enzyme linked immunosorbent assay (ELISA). Moreover, lipid peroxidation product (4-hydroxynonenal (4 HNE))-adducted proteins and advanced glycation end-products (AGE) were immunohistochemically detected in the foam cells of the fatty streaks. These results revealed that diabetic APA hamsters had hyperlipidemia characterized by increases in chylomicron, LDL and abnormal lipoprotein, and suggested that oxidized LDL and/or glycated LDL might be actively uptaken by macrophages and play an important role in the initial stage of atherogenesis.

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Section: Discussionmentioning

confidence: 99%

APA Hamster Model for Diabetic Atherosclerosis. 2. Analysis of Lipids and Lipoproteins.

et al. 2000

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“…In the group of biliary atresia, serum levels of total cholesterol and phospholipids were increased and the levels of the latter were correlated to the Lp-X levels determined by a semiquan titative assay. The fact that the major components of Lp-X are unesterified cholesterol (22%) and phospholipids (66%) (Seidel et al 1969) may provide a reason for correlation between Lp-X and phospholipid levels. However, in Lp-X positive infants with neonatal hepatitis, its level was not related to the values of serum cholesterol or phospholipids; these values were not different from those in Lp-X negative infants with the illness.…”

Section: Discussionmentioning

confidence: 99%

“…serum Recently, lipoprotein-X (Lp-X) which is an abnormal lipoprotein appearing in the serum of obstructive jaundice has been shown to be a reliable parameter of cholestasis (Seidel et al 1969(Seidel et al , 1973a. Since its detection methods are simple and easy, the Lp-X test would be useful as an initial diagnostic tool in obstructive jaundice in early infancy.…”

mentioning

confidence: 99%

Semiquantitative Assay of Serum Lipoprotein-X in Differential Diagnosis of Neonatal Hepatitis and Congenital Biliary Atresia

Tazawa

Konno

1980

Tohoku J. Exp. Med.

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“…These are complexes mainly of phospholipid, unesterified cholesterol and VLDL apoproteins (the delipidated proteins from VLDL, see below) that form in patients with obstructive liver disease. The lipoprotein is separable by Cohn-fractionation and is identified by an antibody that also reacts with apo-VLDL (Seidel et al, 1969). The accompanying lipoprotein pattern is variable.…”

Section: Major Lipoprotein Familiesmentioning

confidence: 99%

Classification of Hyperlipidaemias and Hyperlipoproteinaemias ,

Beaumont¹,

Carlson²,

Cooper³

et al. 1972

Standard Methods of Clinical Chemistry

255

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Many studies ofatherosclerosis have indicated hyperlipidaemia as a predisposing factor to vascular disease. The relationship holds even for mild degrees of hyperlipidaemia, a fact that underlines the importance ofthis category ofdisorders. Both primary and secondary hyperlipidaemias represent such a variety ofabnormalities that an internationally acceptable provisional classification is highly desirable in order to facilitate communication between scientists with different backgrounds.The present memorandum presents such a classification; it briefly describes the criteria for diagnosis ofthe main types ofhyperlipidaemia as well as the methods oftheir determination. Because lipoproteins offer more information than analysis ofplasma lipids (most of the plasma lipids being bound to various proteins), the classification is based on lipoprotein analyses by electrophoresis and ultracentrifugation. Simpler methods, however, such as the observation ofplasma and measurements of cholesterol and triglycerides, are used to the fullest possible extent in determining the lipoprotein patterns.

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A lipoprotein characterizing obstructive jaundice. I. Method for quantitative separation and identification of lipoproteins in jaundiced subjects

Cited by 324 publications

References 34 publications

APA Hamster Model for Diabetic Atherosclerosis. 2. Analysis of Lipids and Lipoproteins.

APA Hamster Model for Diabetic Atherosclerosis. 2. Analysis of Lipids and Lipoproteins.

Semiquantitative Assay of Serum Lipoprotein-X in Differential Diagnosis of Neonatal Hepatitis and Congenital Biliary Atresia

Classification of Hyperlipidaemias and Hyperlipoproteinaemias ,

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