A liquid chromatography/mass spectrometry method for the evaluation of intestinal permeability

Lostia, Alfonso; Lionetto, Luana; Principessa, Luigi; Evangelisti, Melania; Gamba, A.; Villa, Maria Pia; Simmaco, Maurizio

doi:10.1016/j.clinbiochem.2008.03.016

Cited by 48 publications

(53 citation statements)

References 30 publications

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“…With this method, it is possible to detect common saccharides in extracts from plants [27]. Several HPLC procedures are available for the determination of sugars in urine [10,12,16,[28][29][30]. Some methods are based on refractive index detection [28], which has the advantage of being universal.…”

Section: Introductionmentioning

confidence: 99%

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Modern approach for determination of lactulose, mannitol and sucrose in human urine using HPLC–MS/MS for the studies of intestinal and upper digestive tract permeability

Kubica

Kot‐Wasik

Wasik

et al. 2012

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

“…Electrochemical detection was applied for sugars analysis as well [34]. Specific and sensitive mass spectrometry detection in multiple reaction monitoring mode (MRM) combined with HPLC analysis was recently presented for the measurement of these compounds in urine of children affected by abdominal recurrent pain [29].…”

Section: Introductionmentioning

confidence: 99%

Modern approach for determination of lactulose, mannitol and sucrose in human urine using HPLC–MS/MS for the studies of intestinal and upper digestive tract permeability

Kubica

Kot‐Wasik

Wasik

et al. 2012

Journal of Chromatography B

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“…Throughout the world, saccharides with different molecular weights, absorption routes, and levels of urinary excretion have been used as parameters to determine intestinal permeability 100–104…”

Section: Analytic Methods To Determine Intestinal Permeabilitymentioning

confidence: 99%

Intestinal permeability in a patient with liver cirrhosis

Valadez

Rivera-Espinosa

Méndez-Guerrero

et al. 2016

TCRM

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Liver cirrhosis is a worldwide public health problem, and patients with this disease are at high risk of developing complications, bacterial translocation from the intestinal lumen to the mesenteric nodes, and systemic circulation, resulting in the development of severe complications related to high mortality rate. The intestinal barrier is a structure with a physical and biochemical activity to maintain balance between the external environment, including bacteria and their products, and the internal environment. Patients with liver cirrhosis develop a series of alterations in different components of the intestinal barrier directly associated with the severity of liver disease that finally increased intestinal permeability. A “leaky gut” is an effect produced by damaged intestinal barrier; alterations in the function of tight junction proteins are related to bacterial translocation and their products. Instead, increasing serum proinflammatory cytokines and hemodynamics modification, which results in the appearance of complications of liver cirrhosis such as hepatic encephalopathy, variceal hemorrhage, bacterial spontaneous peritonitis, and hepatorenal syndrome. The intestinal microbiota plays a fundamental role in maintaining the proper function of the intestinal barrier; bacterial overgrowth and dysbiosis are two phenomena often present in people with liver cirrhosis favoring bacterial translocation. Increased intestinal permeability has an important role in the genesis of these complications, and treating it could be the base for prevention and partial treatment of these complications.

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“…Examples of potential interference by isomers include the quantification of testosterone (biologically inactive isomer: epitestosterone) (59 ), 2-arachidonoylglycerol (biologically inactive isomer: 1-arachidonoylglycerol ) (60 ), cortisone (synthetic isomer: prednisolone) (61 ), methylmalonic acid (isobaric endogenous metabolite: succinic acid) (41 ), and mannitol (stereoisomer: sorbitol) (62 ). The last example demonstrates that LC-MS/MS may ultimately fail in specifically quantifying known compounds because the separation of these 2 sugars by use of LC is extremely difficult.…”

Section: Pitfalls Of Lc-ms/msmentioning

confidence: 99%

Pitfalls Associated with the Use of Liquid Chromatography–Tandem Mass Spectrometry in the Clinical Laboratory

2010

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BACKGROUND Novel mass spectrometric techniques such as atmospheric pressure ionization and tandem mass spectrometry have substantially extended the spectrum of clinical chemistry methods during the past decade. In particular, liquid chromatography tandem–mass spectrometry (LC-MS/MS) has become a standard tool in research laboratories as well as in many clinical laboratories. Although LC-MS/MS has features that suggest it has a very high analytical accuracy, potential sources of inaccuracy have recently been identified. CONTENT The sources of inaccuracy in LC-MS/MS methods used in the routine quantification of small molecules are described and discussed. Inaccuracy of LC-MS/MS methods can be related to the process of ionization through the insource transformation of conjugate metabolites or target analytes and may also be attributable to ionization matrix effects that have a differential impact on target analytes and internal-standard compounds. Inaccuracy can also be associated with the process of ion selection, which mainly occurs when compounds from the sample matrix share mass transitions with a target analyte. In individual assays, most potential sources of inaccuracy can be controlled by sufficient LC separation–based sample workup before MS analysis. SUMMARY LC-MS/MS methods should undergo rigorous and systematic validation before introduction into patient care.

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A liquid chromatography/mass spectrometry method for the evaluation of intestinal permeability

Cited by 48 publications

References 30 publications

Modern approach for determination of lactulose, mannitol and sucrose in human urine using HPLC–MS/MS for the studies of intestinal and upper digestive tract permeability

Modern approach for determination of lactulose, mannitol and sucrose in human urine using HPLC–MS/MS for the studies of intestinal and upper digestive tract permeability

Intestinal permeability in a patient with liver cirrhosis

Pitfalls Associated with the Use of Liquid Chromatography–Tandem Mass Spectrometry in the Clinical Laboratory

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