This study introduces a state-of-the-art road debris removal system (i.e., automated road debris removal system (ROBOS)) and analyzes its impact on traffic flow using the microscopic simulation model, VISSIM. Robust simulation scenarios were established, and the major parameters of individual scenarios were also included based on extensive interviews with road workers. The performances of road workers and the ROBOS in regard to road debris removal were investigated in terms of four measures of effectiveness. These included queue, density, percentage delay, and number of vehicle stops. To mimic the major arterial roadways in South Korea, two-lane, three-lane, and four-lane roads were considered for implementing the road debris removal process. The simulation results as well as the statistical analysis revealed that the ROBOS outperforms the direct removal by road workers in all aspects. Thus, the ROBOS can remove the debris more safely and efficiently. The findings of this research can improve institutional operational guidelines for the ROBOS by providing an appropriate range for its parameters. This can result in enhancing traffic safety and efficiency. Notwithstanding the simulation results, the more unexpected cases, such as the driver carelessness during removal work, should be reflected in future research to thoroughly prevent the potential risk of accidents as well as maintaining smooth traffic flow.