A longitudinal study of Shiga-toxigenic Escherichia coli (STEC) prevalence in three Australian dairy herds

Cobbold, Rowland N.; Desmarchelier, Patricia

doi:10.1016/s0378-1135(99)00173-x

Cited by 132 publications

(123 citation statements)

References 24 publications

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“…The farm raised post-weaned heifers only. In a previous report, weanlings and heifers showed a higher isolation rate of EHEC O157 compared with those of calves and lactating cattle [7]. EHEC O157 was also isolated from environmental samples, though the isolation rate was low in comparison with that from faeces.…”

Section: Isolation and Prevalence Of Ehec O157 From Faeces Of Heifersmentioning

confidence: 70%

“…The prevalence of EHEC O157 in cattle has been reported to be 0.2-60.4% in the United States [10,13,23,28], 1.9% in Australia [7], and 4.2-66.7% in Europe [5,6,16,24]. In Japan, it was reported that the isolation rate of the EHEC was between 0.3% [12] and 6.5% [15] in faeces samples from animals delivered to a slaughterhouse.…”

Section: Shiga Toxin (Stx)-producing Escherichia Coli (Stec) Ismentioning

confidence: 99%

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A Three-Year Study of Enterohemorrhagic Escherichia coli O157 on a Farm in Japan

Ezawa

Gocho

Saitoh

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. A long-term study was performed on the prevalence of enterohemorrhagic Escherichia coli (EHEC) O157 in bovine faeces. The present study was conducted on heifers raised on a farm showing a high isolation rate of EHEC O157 in previous years. The p revalence of EHEC O157 isolated from faecal samples was 10.6% (222/2104), 5.6% (181/3225), and 5.6% (153/2744) from 1998 to 2000, respectively. The numbers of EHEC O157-positive heifers for the same 3 years were 46.3% (185/400), 36.8% (147/399), and 31.7% (130/410), respectively. The seasonal prevalence of EHEC O157 varied according to the year. Most positive heifers excreted the EHEC O157 only once during the survey, though it was excreted 2 or 3 times by some heifers. The results obtained in the present study showed that the farm examined was heavily contaminated with EHEC O157. It is assumed that EHEC O157 does not remain in individual catt le long-term, but does exist long-term on farms due to repeated infection.

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Section: Isolation and Prevalence Of Ehec O157 From Faeces Of Heifersmentioning

confidence: 70%

Section: Shiga Toxin (Stx)-producing Escherichia Coli (Stec) Ismentioning

confidence: 99%

A Three-Year Study of Enterohemorrhagic Escherichia coli O157 on a Farm in Japan

Ezawa

Gocho

Saitoh

et al. 2004

The Journal of Veterinary Medical Science

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“…Aliquots (1 mL) were taken and centrifuged (12). The supernatant was discarded and the pellet was streaked onto cystine lactose electrolyte deficient agar.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Shigatoxigenic Escherichia coli serogroups O157, O111 and O113 in feces, water and milk samples from dairy farms

Vicente¹,

Amaral²,

Cerqueira³

2005

Braz. J. Microbiol.

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The objective of this study was to determine the prevalence of Shigatoxigenic Escherichia coli (STEC) and STEC serogroups O157, O111 and O113 in feces, water and milk sampled in dairy farms in Jaboticabal, SP, Brazil. Feces (n=454), water (n=54) and milk samples (n=30) were collected from 10 herds and assessed for the presence of the virulence genes stx 1 , stx 2 and eae by polymerase chain reaction (PCR). All stx and eae positive samples were submitted to a second PCR reaction targeting the sequences rfb O157, rfb O111 and rfb O113. High prevalence of stx was detected (59.9%) in fecal samples, whereas the prevalence of sequences rfb O157, rfb O111 and rfb O113 was 18.9%, 3.3% and 30.4%, respectively. All sequences were detected more frequently in calves and heifers. Sequences stx2 and eae were prevalent in the fecal samples. stx sequences were detected in 1.9% and 3.3% of water and milk samples, respectively. Low prevalence of E. coli O113 was observed in water samples, whereas no E. coli O157 or E. coli O111 was detected. Furthermore, none of the serogroups were identified in milk samples. STEC was identified in all herds (100%), and serogroups O157, O111 and O113 were observed in 40%, 50% and 90% of the herds, respectively. In conclusion, the high STEC prevalence detected in dairy herds evidences that bovine feces might play an important role as a contamination source in the region of Jaboticabal, Brazil.

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“…EHEC O157 can be isolated from various animals such as cattle [2,4,5,8,9,12,16,23], sheep [6], deer [1], and so on. In particular, it is often isolated from faeces of cattle showing no clinical signs of disease.…”

mentioning

confidence: 99%

“…In particular, it is often isolated from faeces of cattle showing no clinical signs of disease. It was reported that the prevalence of EHEC O157 in cattle was 0.2-28% in the United States [7,8,23], 1.9% in Australia [5], and 4.2-20% in Europe [2,4,9,16]. Therefore, cattle can harbour the organisms in their faeces and are regarded as a natural reservoir and source of infection [3].…”

mentioning

confidence: 99%

High Prevalence of Enterohemorrhagic Escherichia coli (EHEC) O157 from Cattle in Selected Regions of Japan

Ezawa

Gocho

Kawata

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The prevalence of enterohemorrhagic Escherichia coli (EHEC) O157 was examined in bovine faeces. EHEC O157 was isolated from the faeces of 42 (13.0%) of 324 cattle. Of the 4 farms and the facilities tested, the 3 farms and the facilities wer e found positive for EHEC O157. The highest isolation rate among the farms was 33.7%. The prevalence of EHEC O157 in heifers was higher than that in calves and other cattle. No cattle positive for EHEC O157 showed any clinical signs except 2 calves with diarrhea in a veterinary hospital. Almost all isolates possessed the stx gene, and Stx-positive strains carrying both stx 1 and stx 2 genes were predominant. These results indicate that EHEC O157 are distributed in bovine faeces, and that dairy and beef farms in selected regions of Japan are heavily contaminated with the organisms. KEY WORDS: cattle, EHEC O157, prevalence.

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A longitudinal study of Shiga-toxigenic Escherichia coli (STEC) prevalence in three Australian dairy herds

Cited by 132 publications

References 24 publications

A Three-Year Study of Enterohemorrhagic Escherichia coli O157 on a Farm in Japan

A Three-Year Study of Enterohemorrhagic Escherichia coli O157 on a Farm in Japan

Shigatoxigenic Escherichia coli serogroups O157, O111 and O113 in feces, water and milk samples from dairy farms

High Prevalence of Enterohemorrhagic Escherichia coli (EHEC) O157 from Cattle in Selected Regions of Japan

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