A loop mediated isothermal amplification (LAMP) assay for rapid and reliable detection of Anguina wevelli, a grass parasitic nematode

Yu, Lu-zhen; Song, Shuai; Qi, Long-jun; Yu, Zi-xiang; Jiao, Binbin; Yang, Juan

doi:10.1007/s10658-017-1320-8

Cited by 13 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many other LAMP assays have been developed to detect PPN, such as Aphelenchoides besseyi [42], A. ritzemabosi [43], Anguina wevelli [44] and A. agrostis [45], Radopholus similis, directly from infected plant tissues [46], Ditylenchus destructor from complex plant/nematode DNA mixtures [47] and Tylenchulus semipenetrans in soil samples [48,49].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, Globodera pallida

et al. 2021

View full text Add to dashboard Cite

The potato cyst nematode (PCN) Globodera pallida has acquired significant importance throughout Europe due to its nefarious effects on potato production. Rapid and reliable diagnosis of PCN is critical during the surveillance programs and for the implementation of control measures. Molecular DNA-based methods are available, but they require expensive laboratory facilities, equipment and trained technicians. Moreover, there is an additional need of time for sample shipment and testing. In this work, we have developed a new and simple assay which reliably discriminates G. pallida from other cyst nematodes in less than 40 min. This assay may be applied either on cysts or juveniles with the ability to detect a single juvenile of G. pallida in a sample of at least 40 juveniles of the non-target species G. rostochiensis. This test should be a tool to improve the performance of the laboratory and has the potential to be performed on-site.

show abstract

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, Globodera pallida

et al. 2021

View full text Add to dashboard Cite

show abstract

“…by LAMP assay in soil and roots were obtained at 63 °C for 60 min (Niu et al 2011). For the detection of Anguina wevelli LAMP assay resulted after 45 min at 63°C (Yu et al 2018).…”

Section: Discussionmentioning

confidence: 99%

“…In view of these advantages, this technology has been used commercially in a variety of pathogens detection kits (Mori and Notomi 2009). In the eld of plant nematology, LAMP assays have been developed for the detection of parasitic worms of pine wood Bursaphelenchus xylophilus (Kikuchi et al 2009;Meng et al 2018), coconut red ring worm B. cocophilus (Ide et al 2017), burrowing worm Radopholus similis (Peng et al 2012), citrus root worm Tylenchulus semipenetrans (Song et al 2017), grass pararsitic worm Anguina wevelli (Yu et al 2018) and tropical root-knot worm Meloidogyne incognita, M. enterolobii (Niu et al 2011;Niu et al 2012) as well as the temperate root-knot worm M. hapla (Peng et al 2017), and apple root-knot worm M. mali (Zhou et al 2017). Quick and speci c detection of G. pallida in the soil is imperative for development of management strategy against the particular species.…”

Section: Introductionmentioning

confidence: 99%

Detection of Globodera pallida directly from soil sample using mt-COI region based LAMP assay

Bairwa

Dipta

Verma

et al. 2021

Preprint

View full text Add to dashboard Cite

Potato cyst nematodes (PCN), Globodera rostochiensis (Golden/yellow) and G. pallida (White), are economically important and relatively specialized pest of potato (Solanum tuberosum L.). Both the species are being identified based on cyst colour after 55-60 days after planting (DAP) however, after 65 DAP, we cannot differentiate based on cyst colour as both species turns brown. Moreover, the molecular techniques available to detect the PCN at species level is laborious, time consuming and costly. Therefore, development of rapid, accurate and economically cheap technique for detection of PCN at species level from the field is important to device effective management strategies for sustainable potato production. Accordingly, in the first instance, loop-mediated isothermal amplification (LAMP) assay was developed to detect G. pallida directly from soil by using the mitochondrial (mt-COI) gene specific primer. The LAMP assay was completed within 60 min at 60 °C isothermal conditions and the primer, efficiently detects the G. pallida without any cross reaction with G. rostochiensis, Meloidogyne incognita, M. javanica, Heterodera avenae, H. carotae, and Cactodera spp. In analytical sensitivity tests, the assay was able to detect G. pallida with 1000 times less DNA concentration (10 fg/µl) as compared to conventional PCR (10 pg/µl) and the LAMP product was visualized by using SYBR Gold nucleic acid dye and the assay can be highly useful in detection of G. pallida.

show abstract

“…No additional suitable loop primers were identified using Primer Explorer V5, with the short size of the template as the only limiting factor. However, loop primers have been demonstrated to be not essential for a successful LAMP reaction, although a reduction in amplification times can sometimes be achieved [34][35][36].…”

Section: Design and Selection Of Lamp Primersmentioning

confidence: 99%

A Powerful LAMP Weapon against the Threat of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens

et al. 2020

View full text Add to dashboard Cite

Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is a Gram-positive phytopathogenic bacterium attacking leguminous crops and causing systemic diseases such as the bacterial wilt of beans and bacterial spot of soybeans. Since the early 20th century, Cff is reported to be present in North America, where it still causes high economic losses. Currently, Cff is an emerging plant pathogen, rapidly spreading worldwide and occurring in many bean-producing countries. Infected seeds are the main dissemination pathway for Cff, both over short and long distances. Cff remains viable in the seeds for long times, even in field conditions. According to the most recent EU legislation, Cff is included among the quarantine pests not known to occur in the Union territory, and for which the phytosanitary inspection consists mainly of the visual examination of imported bean seeds. The seedborne nature of Cff combined with the globalization of trades urgently call for the implementation of a highly specific diagnostic test for Cff, to be routinely and easily used at the official ports of entry and into the fields. This paper reports the development of a LAMP (Loop-Mediated Isothermal Amplification) specific for Cff, that allows the detection of Cff in infected seeds, both by fluorescence and visual monitoring, after 30 min of reaction and with a detection limit at around 4 fg/μL of pure Cff genomic DNA.

show abstract

A loop mediated isothermal amplification (LAMP) assay for rapid and reliable detection of Anguina wevelli, a grass parasitic nematode

Cited by 13 publications

References 25 publications

Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, Globodera pallida

Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, Globodera pallida

Detection of Globodera pallida directly from soil sample using mt-COI region based LAMP assay

A Powerful LAMP Weapon against the Threat of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens

Contact Info

Product

Resources

About