A low-cost non instrumental method for semiquantitative determination of mercury in fish

Yallouz, Allegra Viviane; Campos, Reinaldo Calixto de; Paciornik, Sidnei

doi:10.1007/s002160050093

Cited by 19 publications

(18 citation statements)

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“…The experimental conditions are shown in Table 1 and were optimized as described elsewhere. 15 For determining the relationship between the amount of mercury passing through and trapped on the detecting paper, as well as the respective developed color characteristic and intensity, 20 mL of solutions containing increasing mercury masses (up to 6 μg of Hg) were used: SnCl 2 was added as reductant and the Hg 0 formed aerated to the detecting paper. The papers were then analyzed by DIA and, subsequently, by cold vapor AAS, after digestion.…”

Section: Preparation Of Samples For Image Analysismentioning

confidence: 99%

“…In this case, 15 mL of 50% (m/v) SnCl 2 should be used as reductant. 15 The analytical curve was performed with aqueous analytical solutions containing 100, 200, 300, 400, 500 and 600 ng of mercury, and their correspondent color digitally analyzed as described. The papers were not digested, that is a 100% recovery was considered, and a correlation coefficient of 0.997 was observed.…”

Section: Analytical Figures Of Meritmentioning

confidence: 99%

“…14 In a previous work a non-instrumental semi-quantitative method for Hg determination in fish based on a spot test reaction was described. 15 The method aimed at be used in isolated Amazonian communities where few facilities are available and fish is the main protein source. 16 However, spot-tests are normally limited in their applicability because a human operator evaluates the color and only concentration ranges can be discriminated.…”

Section: Introductionmentioning

confidence: 99%

“…More details on this preparation procedure are given elsewhere. 15,18 The covered papers were hermetically stored in a container with controlled humidity (95%).…”

Section: Detecting Papersmentioning

confidence: 99%

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Scanner image analysis in the quantification of mercury using spot-tests

Paciornik¹,

Yallouz²,

Campos³

et al. 2006

J. Braz. Chem. Soc.

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O presente trabalho apresenta um procedimento de análise digital de imagem para a quantificação de mercúrio, baseado no desenvolvimento de uma cor derivada de uma reação de "spot test", utilizando-se um scanner comercial. A análise digital de imagem da cor desenvolvida foi usada para estabelecer uma relação quantitativa entre os componentes da cor e a concentração de mercúrio, sendo a sua intensidade relacionada à concentração na amostra original. Diferentes modelos de separação de cor foram comparados quanto à sensibilidade frente à variação da concentração de mercúrio. O modelo HLS (Hue-Lightness-Saturation) apresentou os melhores resultados, mostrando a relação linear mais sensível entre o valor da componente H (Hue) e a concentração de mercúrio. O procedimento, quando aplicado a amostras de peixes de exercícios interlaboratoriais, apresentou boa correspondência entre os valores encontrados e aqueles relatados. Os limites de detecção foram estimados em função dos intervalos de confiança das curvas analíticas obtidas, mostrando-se fortemente dependentes da faixa de calibração e apresentando um valor de 0,007 μg g -1 na amostra original (peso úmido), para um intervalo de calibração de 100 a 600 ng.A digital image analysis procedure for mercury quantification based on the analysis of the color developed in a spot-test derived reaction using a commercial scanner is described. The color intensity is related to the concentration in the original sample. Digital image analysis of the developed color was used to establish a quantitative relationship between the color components and the mercury concentration. Different color separation models were compared and their sensitivity to the variation of the mercury concentration was evaluated. The Hue-LightnessSaturation (HLS) model provided the best results, showing a linear relationship between the Hue value and the mercury concentration. The procedure was used for the analysis of interlaboratorial fish samples and good concordance between found and reported values was found. The detection limits were estimated from the confidence bands of the analytical curves. For a calibration interval of 100 to 600 ng, a detection limit of 0.007 μg g -1 (wet weight) was found.

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Section: Preparation Of Samples For Image Analysismentioning

confidence: 99%

Section: Analytical Figures Of Meritmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…More details on this preparation procedure are given elsewhere. 15,18 The covered papers were hermetically stored in a container with controlled humidity (95%).…”

Section: Detecting Papersmentioning

confidence: 99%

See 2 more Smart Citations

Scanner image analysis in the quantification of mercury using spot-tests

Paciornik¹,

Yallouz²,

Campos³

et al. 2006

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…For this reason, a wide variety of spectrophotometric methods for the determination of mercury have been reported. [9][10][11][12][13][14][15][16][17][18][19] Each chromogenic system has its advantages and disadvantages with respect to sensitivity, selectivity and analysis speed.…”

Section: Introductionmentioning

confidence: 99%

Solid phase extraction and spectrophotometric determination of mercury in tobacco and tobacco additives with 5-(p-aminobenzylidene)-thiothiorhodanine

Weizhu¹,

Hu²,

Ma³

et al. 2006

J. Braz. Chem. Soc.

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Foi desenvolvido um método altamente sensível, seletivo e rápido para a determinação de mercúrio, a partir da reação rápida de mercúrio(II) com 5-(p-aminobenzilideno)-tiorodanina (ABTR) e posterior extração em fase sólida do quelato colorido, utilizando discos C 18 . Em pH 3,5 e na presença do emulsificante-OP, ABTR reage com mercúrio(II) para formar um quelato vermelho na razão molar 1:2 (mercúrio:ABTR). O quelato foi enriquecido pela extração em fase sólida com discos C 18 e o quelato retido, eluído com dimethyl formamida (DMF). Um fator de enriquecimento na ordem de 50 foi obtido. Em DMF, a absortividade molar do quelato é 1,21×10 5 L mol -1 cm -1 a 545 nm, e a lei de Beer é obedecida no intervalo 0,01~3 μg mL -1 na solução medida. O desvio padrão relativo para onze replicatas a 0,01 μg mL -1 é 1,7%. Este método foi aplicado para a determinação de mercúrio em tabaco e aditivos de tabaco. Bom coeficiente de preconcentração foi encontrado, comparando-se o método proposto com outros similares.A highly sensitive, selective and rapid method for the determination of mercury based on the rapid reaction of mercury(II) with 5-(p-aminobenzylidene)-thiorhodanine (ABTR) and the solid phase extraction of the colored chelate with C 18 disks has been developed. At pH 3.5 and in the presence of emulsifier-OP medium, ABTR reacts with mercury(II) to form a red chelate of a 1:2 (mercury to ABTR) molar ratio. This chelate was enriched by solid phase extraction with C 18 disks and the retained chelate eluted form the disks with dimethyl formamide (DMF). An enrichment factor of 50 was achieved. In the DMF medium, the molar absorptivity of the chelate is 1.21×10 5 L mol -1 cm -1 at 545 nm, and Beer's law is obeyed in the 0.01~3 μg mL -1 range in the measured solution. The relative standard deviation for eleven sample replicate measurements at the 0.01 μg mL -1 level is 1.7%. This method was applied to the determination of mercury in tobacco and tobacco additives and good preconcentration was found between proposed and comparative methods results.

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Study on the Solid Phase Extraction of Hg(II)‐ABR Chelate with C₁₈ Disks and its Application to the Determination of Mercury in Tobacco and Tobacco Additives

Chen¹,

Yang²,

Hu³

et al. 2004

J Chinese Chemical Soc

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A highly sensitive, selective and rapid method for the determination of mercury based on the rapid reaction of mercury(II) with 5-(p-aminobenzylidene)-rhodanine (ABR) and the solid phase extraction of the colored chelate with C 18 disks has been developed. In the presence of pH = 3.5 sodium acetate-acetic acid buffer solution and Emulsifier-OP medium, ABR reacts with mercury(II) to form a red chelate of a molar ratio 1:2 (mercury to ABR). This chelate was enriched by the solid phase extraction with C18 disks and eluted the retained chelate from the disks with dimethyl formamide (DMF). The enrichment factor of 50 was achieved. In the DMF medium, the molar absorptivity of the chelate is 1.16´10 5 L.mol -1 .cm -1 at 540 nm. Beer's law is obeyed in the range of 0.01~3 mg/mL in the measured solution. The relative standard deviation for eleven replicated samples of 0.01 mg/mL level is 1.83%. This method was applied to the determination of mercury in tobacco and tobacco additives with good results.

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A low-cost non instrumental method for semiquantitative determination of mercury in fish

Cited by 19 publications

References 1 publication

Scanner image analysis in the quantification of mercury using spot-tests

Scanner image analysis in the quantification of mercury using spot-tests

Solid phase extraction and spectrophotometric determination of mercury in tobacco and tobacco additives with 5-(p-aminobenzylidene)-thiothiorhodanine

Study on the Solid Phase Extraction of Hg(II)‐ABR Chelate with C₁₈ Disks and its Application to the Determination of Mercury in Tobacco and Tobacco Additives

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A low-cost non instrumental method for semiquantitative determination of mercury in fish

Cited by 19 publications

References 1 publication

Scanner image analysis in the quantification of mercury using spot-tests

Scanner image analysis in the quantification of mercury using spot-tests

Solid phase extraction and spectrophotometric determination of mercury in tobacco and tobacco additives with 5-(p-aminobenzylidene)-thiothiorhodanine

Study on the Solid Phase Extraction of Hg(II)‐ABR Chelate with C18 Disks and its Application to the Determination of Mercury in Tobacco and Tobacco Additives

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Product

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About

Study on the Solid Phase Extraction of Hg(II)‐ABR Chelate with C₁₈ Disks and its Application to the Determination of Mercury in Tobacco and Tobacco Additives