A Low noise CMOS image sensor with an emission filter for fluorescence applications

Abstract

This paper presents a 128x128 low noise CMOS image sensor with emission filter for fluorescence detection. The imager, fabricated in 0.18µm CMOS technology, provides lownoise operation by employing both the active reset (AR) technique and the active column sensor (ACS) readout method. The emission filter was fabricated using PDMS and Sudan II Blue dye mixed, spin-coated and deposited in the class 1000 clean room. The designed filter is suitable for excitation at wavelengths below 340 nm and emission at 450nm and above. Filter properties, such as thickness, transmission and efficiency of utilization with the fabricated imager are discussed. Preliminary measurements of the system using microbeads are also presented.

Gene expression analysis with an integrated CMOS microarray by time-resolved fluorescence detection

“…These optical or spectral differentiation techniques can deliver at best 60dB of background rejection (Dandin M. 2007), which is considered inadequate for practical applications. These active substrates (Thrush, Levi et al 2003; Fixe, Chu et al 2004; Novak, Neuzil et al 2007; Beiderman, Tam et al 2008; Jang, Cao et al 2009) have consisted of simple photodiodes (Thrush, Levi et al 2003; Fixe, Chu et al 2004) or more complex imager arrays (Parikh, Gulak et al 2007; Schwartz, Gong et al 2008). In some cases, fluorescence detection is avoided through the use of bioluminescence (Eltoukhy, Salama et al 2006) or radioactive markers (Salin, Tujasinovic et al 2002).…”

A CMOS/Thin-Film Fluorescence Contact Imaging Microsystem for DNA Analysis

“…The applications include brain neural activity monitoring [18], particle and pathogen detection [19], [20], and DNA detection [10]. The filters in these designs are generally fabricated directly on the surface of a CMOS die.…”

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