A Lower Proportion of Regulatory B Cells in Patients with Henoch–Schoenlein Purpura Nephritis

Abstract

BackgroundHenoch—Schoenlein purpura is the one of most common types of systemic vasculitis that involves impaired renal function and Henoch-Schoenlein purpura nephritis (HSPN). The diagnosis of this condition is largely based on immunohistologic detection of immunoglobulin A1-containing immune complex in the glomerular deposits of mesangium. Despite clinical advances, the etiopathogenesis of HSPN is still largely unknown.MethodsIn this study, we enrolled 25 newly diagnosed HSPN patients and 14 healthy controls. Then, fractions of B cell subtypes were determined in venous blood using flow cytometry. The serum interleukin (IL)-10 concentration was determined by enzyme-linked immunosorbent assay.ResultsCompared to those in healthy controls, the numbers of CD38+CD19+, CD86+CD19+, CD38+CD86+CD19+, and CD95+CD19+ B cells per microliter of blood were significantly higher in HSPN patients. In contrast, the numbers of CD5+CD19+, IL-10+CD19+, CD5+CD1d+CD19+, and IL-10+CD5+CD1d+CD19+ B cells per microliter of blood and the serum IL-10 concentration were significantly lower in HSPN patients. Following treatment, the numbers of CD38+CD19+ and CD86+CD19+ B cells per microliter of blood were significantly reduced in HSPN patients. However, the numbers of CD5+CD1d+CD19+, CD5+CD1d+IL-10+CD19+, and IL-10+CD19+ B cells per microliter of blood and the serum IL-10 concentration were significantly increased in HSPN patients following treatment. The estimated glomerular filtration rate (eGFR) was negatively correlated with the number of CD38+CD19+ B cells but positively correlated with the numbers of IL-10+CD19+, CD1d+CD5+CD19+, and IL-10+CD1d+CD5+CD19+B cells per microliter of blood and the serum IL-10 concentration. The 24-h urinary protein concentration was positively correlated with the number of CD38+CD19+B cells but negatively correlated with the numbers of IL-10+CD19+, CD1d+CD5+CD19+, and IL-10+CD1d+CD5+CD19+B cells per microliter of blood and the serum IL-10 concentration.ConclusionOur results suggest that CD38+CD19+ and CD1d+CD5+CD19+ B cells (Bregs) contribute to the pathogenesis of HSPN.

Regulatory B cells: the cutting edge of immune tolerance in kidney transplantation

“…The amounts of IL-10 + CD19 + and IL-10 + CD5 + CD1d + B cells, as well as the serum IL-10 concentration were significantly lower in Henoch–Schonlein purpura nephritis patients compared with healthy controls (HC); after treatment, they significantly increased. Meanwhile, the estimated glomerular filtration rate was also positively correlated with the amount of Bregs and the serum IL-10 concentration 54 . Although markers of Bregs might differ, a similar decrease or impairment of IL-10-producing B cells was also observed in patients with rheumatoid arthritis 44 , 55 – 57 , systemic lupus erythematosus (SLE) 24 , 55 , 58 , 59 , multiple sclerosis 26 , 55 , systemic sclerosis 60 , Myasthenia Gravis 61 , and other autoimmune diseases.…”

Effect of Montmorillonite powder on intestinal mucosal barrier in children with abdominal Henoch–Schonlein purpura

“…[ 1 ] HSP is one of the most common small vasculitis in childhood, with clinical manifestations including nonthrombocytopenic purpura, arthritis or arthralgia, abdominal pain, gastrointestinal bleeding and so on. [ 2 , 3 ] Skin purpura and digestive tract symptoms are the most common complains in patients with this disease. [ 4 ] In the past decades, the incidence and recurrence of HSP were gradually increasing, which has invoked great interest of researchers globally.…”

“…[ 4 ] In the past decades, the incidence and recurrence of HSP were gradually increasing, which has invoked great interest of researchers globally. [ 2 – 5 ] In recent years, a large number of studies have shown that the intestinal tract of children with HSP has varying degrees of congestion, edema, erosion, ulcers, bleeding, and other morphological changes. [ 6 – 8 ]…”

Ураження Нирок При Геморагічному Васкуліті Шенлейна — Геноха, Що Почався В Дитячому І Дорослому Віці

“…При ГВ выполнение нефробиопсии показано для оценки прогнозирования течения хронической болезни почек [12]. Как хорошо известно, наиболее типичным морфологическим признаком ГГН является наличие депозитов IgA в эндотелии капилляров и мезангиуме клубочков [11], а также гломерулярные отложения IgG, IgM, С3, C1q [18] и инфильтрация почечных структур клетками с рецепцией CD45 (фодрина) и CD68 (гликопротеина макросиалина) [14]. У взрослых больных примерно в 1/4-1/3 случаев ГГН встречается III морфологический тип (класс), тогда как у детей превалирует ІІ [17,24].…”