A magnetoimpedance biosensor microfluidic platform for detection of glial fibrillary acidic protein in blood for acute stroke classification

Sayad, Abkar; Uddin, Shah Mukim; Yao, Scarlett; Wilson, H. A.; Chan, Jianxiong; Zhao, Henry; Donnan, Geoffrey A; Davis, Stephen M.; Skafidas, Efstratios; Yan, Bernard; Kwan, Patrick

doi:10.1016/j.bios.2022.114410

Cited by 16 publications

(4 citation statements)

References 45 publications

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“…The results of RT-qPCR showed that these four characteristic genes were significantly upregulated in a mouse MCAO model, which verified our results. Glial fibrillary acidic protein (GFAP), an intermediate filament protein only produced by astrocytes, is a well-established marker of astrocyte activation in central nervous system (CNS) diseases ( Sayad et al, 2022 ). Growing evidence suggests the potential clinical application value of blood GFAP levels in numerous neuroinflammatory and neurodegenerative diseases, as they can be used to detect even subtle injury to the CNS ( Abdelhak et al, 2022 ; Heimfarth et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Expression pattern and clinical value of Key RNA methylation modification regulators in ischemic stroke

et al. 2022

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Ischemic stroke (IS) is one of the major causes of death and disability worldwide, and effective diagnosis and treatment methods are lacking. RNA methylation, a common epigenetic modification, plays an important role in disease progression. However, little is known about the role of RNA methylation modification in the regulation of IS. The aim of this study was to investigate RNA methylation modification patterns and immune infiltration characteristics in IS through bioinformatics analysis. We downloaded gene expression profiles of control and IS model rat brain tissues from the Gene Expression Omnibus database. IS profiles were divided into two subtypes based on RNA methylation regulators, and functional enrichment analyses were conducted to determine the differentially expressed genes (DEGs) between the subtypes. Weighted gene co-expression network analysis was used to explore co-expression modules and genes based on DEGs. The IS clinical diagnosis model was successfully constructed and four IS characteristic genes (GFAP, GPNMB, FKBP9, and CHMP5) were identified, which were significantly upregulated in IS samples. Characteristic genes were verified by receiver operating characteristic curve and real-time quantitative PCR analyses. The correlation between characteristic genes and infiltrating immune cells was determined by correlation analysis. Furthermore, GPNMB was screened using the protein-protein interaction network, and its regulatory network and the potential therapeutic drug chloroquine were predicted. Our finding describes the expression pattern and clinical value of key RNA methylation modification regulators in IS and novel diagnostic and therapeutic targets of IS from a new perspective.

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Section: Discussionmentioning

confidence: 99%

Expression pattern and clinical value of Key RNA methylation modification regulators in ischemic stroke

et al. 2022

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“…The meander configuration can use the space of the element and consequently has a higher spatial resolution (Kikuchi et al, 2020). Meanwhile, meanders can be miniaturized and integrated by microelectromechanical systems (Feng et al, 2019b;Sayad et al, 2022;Wang et al, 2019c;Yang et al, 2019). Thin-film meanders were first concerned due to their compatibility with integrated electronic circuits, miniaturization and high spatial resolution.…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, meanders can be miniaturized and integrated by microelectromechanical technology for constructing integrated magnetic detection systems (Feng et al. , 2019b; Sayad et al. , 2022; Wang et al.…”

Section: Introductionmentioning

confidence: 99%

Line spacing effect on the giant magnetoimpedance behavior on microribbon with meander type: a comparison of theory and experiment

Wang,

Yang,

Liu

et al. 2024

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Purpose Microribbon with meander type based on giant magnetoimpedance (GMI) effect has become a research hot spot due to their higher sensitivity and spatial resolution. The purpose of this paper is to further optimize the line spacing to improve the performance of meanders for sensor application. Design/methodology/approach The model of GMI effect of microribbon with meander type is established. The effect of line spacing (Ls) on GMI behavior in meanders is analyzed systematically. Findings Comparison of theory and experiment indicates that decreasing the line spacing increases the negative mutual inductance and a consequent increase in the GMI effect. The maximum value of the GMI ratio increases from 69% to 91.8% (simulation results) and 16.9% to 51.4% (experimental results) when the line spacing is reduced from 400 to 50 µm. The contribution of line spacing versus line width to the GMI ratio of microribbon with meander type was contrasted. This behavior of the GMI ratio is dominated by the overall negative contribution of the mutual inductance. Originality/value This paper explores the effect of line spacing on the GMI ratio of meander type by comparing the simulation results with the experimental results. The superior line spacing is found in the identical sensing area. The findings will contribute to the design of high-performance micropatterned ribbon with meander-type GMI sensors and the establishment of a ribbon-based magnetic-sensitive biosensing system.

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“…Finally, thin film can be miniaturized and developed by micro-electro-mechanical (MEMS) technology such as magnetron sputtering and micro-electroplating, and integrated with circuits easily. Recent observation of multilayered thin film meanders has tended to focus on biomedical sensing [ 4 , 5 , 6 , 7 ], nondestructive testing [ 8 , 9 ], and pressure detection [ 10 ] based on the ultrasensitive and easy-to-implement GMI effect.…”

Section: Introductionmentioning

confidence: 99%

Giant Magnetoimpedance Effect of Multilayered Thin Film Meanders Formed on Flexible Substrates

et al. 2023

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The giant magnetoimpedance effect of multilayered thin films under stress has great application prospects in magnetic sensing, but related studies are rarely reported. Therefore, the giant magnetoimpedance effects in multilayered thin film meanders under different stresses were thoroughly investigated. Firstly, multilayered FeNi/Cu/FeNi thin film meanders with the same thickness were manufactured on polyimide (PI) and polyester (PET) substrates by DC magnetron sputtering and MEMS technology. The characterization of meanders was analyzed by SEM, AFM, XRD, and VSM. The results show that multilayered thin film meanders on flexible substrates also have the advantages of good density, high crystallinity, and excellent soft magnetic properties. Then, we observed the giant magnetoimpedance effect under tensile and compressive stresses. The results show that the application of longitudinal compressive stress increases the transverse anisotropy and enhances the GMI effect of multilayered thin film meanders, while the application of longitudinal tensile stress yields the opposite result. The results provide novel solutions for the fabrication of more stable and flexible giant magnetoimpedance sensors, as well as for the development of stress sensors.

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A magnetoimpedance biosensor microfluidic platform for detection of glial fibrillary acidic protein in blood for acute stroke classification

Cited by 16 publications

References 45 publications

Expression pattern and clinical value of Key RNA methylation modification regulators in ischemic stroke

Expression pattern and clinical value of Key RNA methylation modification regulators in ischemic stroke

Line spacing effect on the giant magnetoimpedance behavior on microribbon with meander type: a comparison of theory and experiment

Giant Magnetoimpedance Effect of Multilayered Thin Film Meanders Formed on Flexible Substrates

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