A Maize Gene Encoding an NADPH Binding Enzyme Highly Homologous to Isoflavone Reductases Is Activated in Response to Sulfur Starvation

Petrucco, Stefania; Bolchi, Angelo; Foroni, Chiara; Percudani, Riccardo; Rossi, Gian Luigi; Ottonello, Simone

doi:10.2307/3870069

Cited by 36 publications

(43 citation statements)

References 38 publications

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“…In monocots such as wheat, lignans are mostly located in the aleurone layer of seeds [5], and in eudicots such as forsthysia, lignans occur in the fruits and stems [6].…”

Section: Introductionmentioning

confidence: 99%

Enhancing lignan biosynthesis by over‐expressing pinoresinol lariciresinol reductase in transgenic wheat

Ayella

Trick

Wang³

2007

Molecular Nutrition Food Res

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Abbreviations: HPLC, high performance liquid chromatography; MS, mass spectrum; PCR, polymerase chain reaction; PLR, pinoresinol lariciresinol reductase; SDG, secoisolariciresinol diglucoside; Ubi, ubiquitin. were found to be 2.2-times higher in one of the three positive transgenic sub-lines at TB 2 B than that in the wild-type (117.9 ± 4.5 vs. 52.9 ± 19.8 µg/g, p < 0.005). To the best of our knowledge, this is the first study that elevated lignan levels in a transgenic wheat line has been successfully achieved through genetic engineering of over-expressed PLR gene. Although future studies are needed for a stably expression and more efficient transformants, the new wheat line with significantly higher SDG contents obtained from this study may have potential application in providing additive health benefits for cancer prevention.

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“…In monocots such as wheat, lignans are mostly located in the aleurone layer of seeds [5], and in eudicots such as forsthysia, lignans occur in the fruits and stems [6].…”

Section: Introductionmentioning

confidence: 99%

Enhancing lignan biosynthesis by over‐expressing pinoresinol lariciresinol reductase in transgenic wheat

Ayella

Trick

Wang³

2007

Molecular Nutrition Food Res

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“…Interestingly, glyoxalase II (spot 4305) was more abundant in 'Frida' at 0, 2, and 42 d exhibiting a 4-, 9-, and 10-foldhigher levels, respectively. The IFR-related protein (spot 1423), exhibiting 2-fold higher levels in 'Jonsok' than 'Frida' at all time points, may act in preservation of reductants or synthesis of antioxidants (Petrucco et al, 1996).…”

Section: Antioxidative and Detoxification Proteinsmentioning

confidence: 99%

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Koehler

Wilson²,

Goodpaster

et al. 2012

Plant Physiology

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To gain insight into the molecular basis contributing to overwintering hardiness, a comprehensive proteomic analysis comparing crowns of octoploid strawberry (Fragaria 3 ananassa) cultivars that differ in freezing tolerance was conducted. Four cultivars were examined for freeze tolerance and the most cold-tolerant cultivar ('Jonsok') and least-tolerant cultivar ('Frida') were compared with a goal to reveal how freezing tolerance is achieved in this distinctive overwintering structure and to identify potential cold-tolerance-associated biomarkers. Supported by univariate and multivariate analysis, a total of 63 spots from twodimensional electrophoresis analysis and 135 proteins from label-free quantitative proteomics were identified as significantly differentially expressed in crown tissue from the two strawberry cultivars exposed to 0-, 2-, and 42-d cold treatment. Proteins identified as cold-tolerance-associated included molecular chaperones, antioxidants/detoxifying enzymes, metabolic enzymes, pathogenesis-related proteins, and flavonoid pathway proteins. A number of proteins were newly identified as associated with cold tolerance. Distinctive mechanisms for cold tolerance were characterized for two cultivars. In particular, the 'Frida' cold response emphasized proteins specific to flavonoid biosynthesis, while the more freezing-tolerant 'Jonsok' had a more comprehensive suite of known stress-responsive proteins including those involved in antioxidation, detoxification, and disease resistance. The molecular basis for 'Jonsok'-enhanced cold tolerance can be explained by the constitutive level of a number of proteins that provide a physiological stress-tolerant poise. Strawberry (Fragaria 3 ananassa) cultivation predominates in regions with mild winters. In colder climates, overwintering hardiness is an essential trait for strawberry cultivation. Freezing injury of strawberry plants is one of the greatest factors in reducing crop yield and quality in temperate regions. Winter damage in Norway, for example, on average causes losses of 20%. Thus, production of cultivars with improved freezing hardiness is one of Norway's major objectives for their strawberry breeding programs. Improvement of cold hardiness is desirable for securing economic sustainability of the existing crops, and for expanding the growing regions of temperate fruit crops. Because strawberry is a representative species for the Rosaceae crops (e

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“…In addition, sulfur-limited plants preferentially express proteins having few Cys and Met residues. These changes have been shown to occur for seed storage proteins and vascular plant enzymes that function in secondary metabolism (Naito et al, 1994;Petrucco et al, 1996). Plant and algal cells also have high levels of sulfur-containing lipids such as sulfoquinovosyl diacylglycerol, which may be important for photosynthetic function under certain environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The induction of genes encoding ATP sulfurylase (Ats1), adenosine 5Ј-phosphosulfate reductase (adenosine 5Ј-phosphosulfate sulfotransferase), and Ser acetyltransferase during sulfur-limited growth may facilitate reduction of sulfate and the synthesis of Cys and Met (Gutierrez-Marcos et al, 1996;Setya et al, 1996;Yildiz et al, 1996;Takahashi et al, 1997). Sulfur limitation may also result in functional substitutions of proteins rich in sulfur with those having few sulfur-containing amino acids (Naito et al, 1994;Petrucco et al, 1996) and provoke protein degradation and recapture of the sulfur contained in Cys and Met (Collier and Grossman, 1992; Ferreira and Teixeira, 1992).The acclimation of C. reinhardtii to sulfur limitation has been well characterized recently and has facilitated the isolation of mutants defective for the synthesis of the extracellular arylsulfatase and elevated sulfate uptake upon imposition of limitation conditions (Davies et al, 1994(Davies et al, , 1996(Davies et al, , 1999. One of the mutants was defective in a gene designated SacI, which encodes a polypeptide that resembles an integral membrane, nutrient transporter (Davies and Grossman, 1998).…”

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confidence: 99%

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confidence: 99%

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Sulfur Economy and Cell Wall Biosynthesis during Sulfur Limitation of Chlamydomonas reinhardtii

2001

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We have identified two novel periplasmic/cell wall polypeptides that specifically accumulate during sulfur limitation of Chlamydomonas reinhardtii. These polypeptides, present at high levels in the extracellular polypeptide fraction from a sulfur-deprived, cell wall-minus C. reinhardtii strain, have apparent molecular masses of 76 and 88 kD and are designated Ecp76 and Ecp88. N-terminal sequences of these polypeptides facilitated the isolation of full-length Ecp76 and Ecp88 cDNAs. Ecp76 and Ecp88 polypeptides are deduced to be 583 and 595 amino acids, respectively. Their amino acid sequences are similar to each other, with features characteristic of cell wall-localized hydroxyproline-rich glycoproteins; the N terminus of each polypeptide contains a predicted signal sequence, whereas the C terminus is rich in proline, alanine, and serine. Ecp76 and Ecp88 have either no (Ecp88) or one (Ecp76) sulfur-containing amino acid and transcripts encoding these polypeptides are not detected in cultures maintained on complete medium, but accumulate when cells are deprived of sulfur. This accumulation is temporally delayed relative to the accumulation of sulfur stress-induced arylsulfatase and ATP sulfurylase transcripts. The addition of sulfate back to sulfur-starved cultures caused a rapid decline in Ecp76 and Ecp88 mRNAs (half lives Ͻ 10 min). Furthermore, the C. reinhardtii sac1 mutant, which lacks a regulatory protein critical for acclimation to sulfur limitation, does not accumulate Ecp76 or Ecp88 transcripts. These results suggest that the Ecp76 and Ecp88 genes are under SacI control, and that restructuring of the C. reinhardtii cell wall during sulfur limitation may be important for redistribution of internal and efficient utilization of environmental sulfur-containing molecules.Sulfur is an essential macronutrient that is taken up by plant, algal, and microbial cells, primarily as the inorganic sulfate anion. Limitations for sulfur in the environment can inhibit plant growth and productivity (Grossman and Takahashi, 2001). However, plants are able to acclimate to sulfur-limited growth conditions by synthesizing enzymes that function in the efficient acquisition and utilization of both external and internal sulfur sources. Sulfur limitation may promote expression of genes encoding sulfatases and high-affinity sulfate transporters in both plants and algae (de Hostos et al., 1989;Yildiz et al., 1994;Smith et al., 1997;Takahashi et al., 2000). In Chlamydomonas reinhardtii and Volvox carteri, an extracellular arylsulfatase (encoded by the Ars1 and Ars2 genes in C. reinhardtii) can facilitate utilization of exogenous, esterified sulfate (de Hostos et al., 1988; Hallman and Sumper, 1994;Davies and Grossman, 1998). The induction of genes encoding ATP sulfurylase (Ats1), adenosine 5Ј-phosphosulfate reductase (adenosine 5Ј-phosphosulfate sulfotransferase), and Ser acetyltransferase during sulfur-limited growth may facilitate reduction of sulfate and the synthesis of Cys and Met (Gutierrez-Marcos et al., 1996;Setya et al., 199...

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A Maize Gene Encoding an NADPH Binding Enzyme Highly Homologous to Isoflavone Reductases Is Activated in Response to Sulfur Starvation

Cited by 36 publications

References 38 publications

Enhancing lignan biosynthesis by over‐expressing pinoresinol lariciresinol reductase in transgenic wheat

Enhancing lignan biosynthesis by over‐expressing pinoresinol lariciresinol reductase in transgenic wheat

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Sulfur Economy and Cell Wall Biosynthesis during Sulfur Limitation of Chlamydomonas reinhardtii

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