Enhancing lignan biosynthesis by over‐expressing pinoresinol lariciresinol reductase in transgenic wheat

Abstract

Abbreviations: HPLC, high performance liquid chromatography; MS, mass spectrum; PCR, polymerase chain reaction; PLR, pinoresinol lariciresinol reductase; SDG, secoisolariciresinol diglucoside; Ubi, ubiquitin. were found to be 2.2-times higher in one of the three positive transgenic sub-lines at TB 2 B than that in the wild-type (117.9 ± 4.5 vs. 52.9 ± 19.8 µg/g, p < 0.005). To the best of our knowledge, this is the first study that elevated lignan levels in a transgenic wheat line has been successfully achieved through genetic engineering of over-expressed PLR gene. Although future studies are needed for a stably expression and more efficient transformants, the new wheat line with significantly higher SDG contents obtained from this study may have potential application in providing additive health benefits for cancer prevention.

Cytostatic inhibition of cancer cell growth by lignan secoisolariciresinol diglucoside

“…Based 217 upon our previous experience in flavonoid apigenin [26] and lignan analysis [9][10]27] those potential metabolites in this moment. Although SDG and/or its metabolites were not 225 detectable in the treated cells, it should be noted that it was not expected for intact SDG to be 226 absorbed, since a phytochemical glycoside is usually deglycosilation by β-glucosidase or lactase 227 phlorozin hydrolase [28].…”

Wheat Mds-1 encodes a heat-shock protein and governs susceptibility towards the Hessian fly gall midge

“…PCR product was directionally inserted into pENTR/ D-TOPO. The final RNAi construct was made by recombination from an LR clonase reaction using a Gateway LR clonase enzyme mix between the entry vector carrying the Mds-1 fragment and the pANDA-mini vector 56 , from which the identical Mds-1 fragment was inserted into both sides of the 920-bp GUS linker in antisense and sense orientations. The transcribed RNA contains a hairpin that forms dsRNA.…”

“…For silencing, the pANDA construct and pAHC20 containing the bar gene were co-bombarded with 1:1 ratio into embryogenic calli as described by Altpeter 58 and modified by Ayella et al 56 Briefly, embryos (2-5 mm) were then excised from immature seeds and plated on CM4 media to initiate the formation of somatic embryo for 2-7 days. Somatic embryos were selected for highly embryogenic calli and were co-bombarded with pAHC20 and GOI plasmids at 1:1 ratio by using the particle inflow gun.…”

A comprehensive review on the phytochemical and pharmacological aspects of Podophyllum hexandrum: a high value medicinal plant

“…Quite recently accumulation of a large amount of pinoresinol has been achieved by pathway engineering in F. koreana. Overexpression of the F. intermedia PLR gene in wheat results in an increase of an endogenous dibenzylbutane-class lignan, Secoisolariciresinol diglucoside (SDG) [59]. The transgenic periwinkle (Catharanthus roseus) cell culture, expressing an alkaloid biosynthetic gene with re-engineered substrate specificity, acquired the capacity to produce various exogenous alkaloids when co-cultured with the substrates [60].…”