A Maize Glycine-Rich Protein Is Synthesized in the Lateral Root Cap and Accumulates in the Mucilage1

Matsuyama, Takashi; Satoh, Hidetaka; Yamada, Yasuyuki; Hashimoto, Takashi

doi:10.1104/pp.120.3.665

Cited by 41 publications

(19 citation statements)

References 41 publications

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“…The release of an array of newly synthesized proteins from living cells, free of microbial contamination, confirmed the secretion of proteins from the pea root cap during border cell separation (Brigham et al, 1995). In recent years the secretion of several specific endogenous proteins from the root cap, including a Gly-rich glycoprotein and ribosome inactivating proteins, has been documented (Matsuyama et al, 1999;Park et al, 2002). Basu et al (2006) used MudPIT and LC-MS/MS to document that 52 extracytosolic proteins are released from roots of Arabidopsis grown in liquid shake culture for 11 d. There was little overlap among the proteins identified in that study and those in the pea root cap secretome (Table II).…”

Section: Discussionmentioning

confidence: 75%

Extracellular Proteins in Pea Root Tip and Border Cell Exudates

et al. 2006

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Newly generated plant tissue is inherently sensitive to infection. Yet, when pea (Pisum sativum) roots are inoculated with the pea pathogen, Nectria haematococca, most newly generated root tips remain uninfected even though most roots develop lesions just behind the tip in the region of elongation. The resistance mechanism is unknown but is correlated spatially with the presence of border cells on the cap periphery. Previously, an array of .100 extracellular proteins was found to be released while border cell separation proceeds. Here we report that protein secretion from pea root caps is induced in correlation with border cell separation. When this root cap secretome was proteolytically degraded during inoculation of pea roots with N. haematococca, the percentage of infected root tips increased from 4% 6 3% to 100%. In control experiments, protease treatment of conidia or roots had no effect on growth and development of the fungus or the plant. A complex of .100 extracellular proteins was confirmed, by multidimensional protein identification technology, to comprise the root cap secretome. In addition to defense-related and signaling enzymes known to be present in the plant apoplast were ribosomal proteins, 14-3-3 proteins, and others typically associated with intracellular localization but recently shown to be extracellular components of microbial biofilms. We conclude that the root cap, long known to release a high molecular weight polysaccharide mucilage and thousands of living cells into the incipient rhizosphere, also secretes a complex mixture of proteins that appear to function in protection of the root tip from infection.

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Section: Discussionmentioning

confidence: 75%

Extracellular Proteins in Pea Root Tip and Border Cell Exudates

et al. 2006

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“…1 GRPs with a putative N-terminal signal peptide are usually located in the cell wall or the cell wall-membrane interface (Sachetto-Martins et al 2000); 2 expression of the GRP genes is highly organ or tissue-specific-usually vascular tissue-specific (Keller et al 1988;Matsuyama et al 1999); and 3 expression of the GRP genes is regulated developmentally and is induced by factors such as plant hormones, the circadian clock, and light and biotic or abiotic stresses such as salt, water, wounding, and pathogens (Ringli et al 2001;Sachetto-Martins et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Root and vascular tissue-specific expression of glycine-rich protein AtGRP9 and its interaction with AtCAD5, a cinnamyl alcohol dehydrogenase, in Arabidopsis thaliana

Chen

Zhong

et al. 2007

J Plant Res

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The vascular tissue of roots performs essential roles in the physical support and transport of water, nutrients, and signaling molecules in higher plants. The molecular mechanisms underlying the function of root vascular tissue are poorly understood. In this study, we analyzed the expression pattern of AtGRP9, a salt stress-responsive gene encoding a glycine-rich protein, and its interacting partner, in Arabidopsis thaliana. Analysis of GUS or GFP expression under the control of the AtGRP9 promoter showed that AtGRP9 was expressed in the vascular tissue of the root; subcellular localization analysis further demonstrated that AtGRP9 proteins were localized in the cell wall and in the cytoplasm. Yeast two-hybrid analysis revealed that AtGRP9 interacted with AtCAD5, a major cinnamyl alcohol dehydrogenase (CAD) involved in lignin biosynthesis, for which tissue-specific distribution was comparable with that of AtGRP9. These results suggest that AtGRP9 may be involved in lignin synthesis in response to salt stress as a result of its interaction with AtCAD5 in A. thaliana.

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“…Two GRP isoforms, GRP-1 (transcripts 185 and 1723) and GRP-2 (transcripts 430, 446, 1112, and 1539), showed ‫%06ف‬ amino acid sequence identity to the maize ZmGRP4 in overlapping regions. ZmGRP4 has been localized to the root cap, but its function is unknown (Matsuyama et al, 1999). One hypothesis is that this class of transcripts might function as an immediate defense to stress, for example, by strengthening cell walls.…”

Section: The Time-specific Response Of Transcripts In Pokkalimentioning

confidence: 99%

Gene Expression Profiles during the Initial Phase of Salt Stress in Rice

et al. 2001

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Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis to one tenth of the prestress value within minutes. Hybridizations of RNA to microarray slides probed for changes in transcripts from 15 min to 1 week after salt shock. Beginning 15 min after the shock, Pokkali showed upregulation of transcripts. Approximately 10% of the transcripts in Pokkali were significantly upregulated or downregulated within 1 hr of salt stress. The initial differences between control and stressed plants continued for hours but became less pronounced as the plants adapted over time. The interpretation of an adaptive process was supported by the similar analysis of salinity-sensitive rice (var IR29), in which the immediate response exhibited by Pokkali was delayed and later resulted in downregulation of transcription and death. The upregulated functions observed with Pokkali at different time points during stress adaptation changed over time. Increased protein synthesis and protein turnover were observed at early time points, followed by the induction of known stress-responsive transcripts within hours, and the induction of transcripts for defenserelated functions later. After 1 week, the nature of upregulated transcripts (e.g., aquaporins) indicated recovery.

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A Maize Glycine-Rich Protein Is Synthesized in the Lateral Root Cap and Accumulates in the Mucilage1

Cited by 41 publications

References 41 publications

Extracellular Proteins in Pea Root Tip and Border Cell Exudates

Extracellular Proteins in Pea Root Tip and Border Cell Exudates

Root and vascular tissue-specific expression of glycine-rich protein AtGRP9 and its interaction with AtCAD5, a cinnamyl alcohol dehydrogenase, in Arabidopsis thaliana

Gene Expression Profiles during the Initial Phase of Salt Stress in Rice

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