1992
DOI: 10.1007/bf00040604
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A major barley allergen associated with baker's asthma disease is a glycosylated monomeric inhibitor of insect ?-amylase: cDNA cloning and chromosomal location of the gene

Abstract: A 14.5 kDa barley endosperm protein that is a major allergen in baker's asthma disease, as previously shown by both in vitro (IgE binding) and in vivo tests, has been identified as a glycosylated monomeric member of the multigene family of inhibitors of alpha-amylase/trypsin from cereals. A cDNA encoding this allergen (renamed BMAI-1) has been isolated and characterized. The deduced sequence for the mature protein, which is 132 residues long, is identical in its N-terminal end to the 20 amino acid partial sequ… Show more

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Cited by 56 publications
(33 citation statements)
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“…Both components are rye members of the BMAI-1 (a barley monomeric inhibitor which does not seem to have the corresponding wheat homologue) subfamily [6]. However, components 1 and 2 behave as homodimeric proteins and, unlike BMAI-1 [12], are not glycosylated. This last difference is likely to be responsible for the strong IgE-binding of BMAI-1 when tested with sera of allergic patients, in contrast with the lack of response of the rye components.…”
Section: Discussionmentioning
confidence: 99%
“…Both components are rye members of the BMAI-1 (a barley monomeric inhibitor which does not seem to have the corresponding wheat homologue) subfamily [6]. However, components 1 and 2 behave as homodimeric proteins and, unlike BMAI-1 [12], are not glycosylated. This last difference is likely to be responsible for the strong IgE-binding of BMAI-1 when tested with sera of allergic patients, in contrast with the lack of response of the rye components.…”
Section: Discussionmentioning
confidence: 99%
“…In wheat and barley the glycosylated forms of WTAI-CM16, BTAICMb and BMAI-1 have been found to be the most prominent allergens, both in vi tro and in vivo (García-Casado et al, 1995;Sanchez-Monge et al, 1992). cDNA clones encoding these three proteins have also been isolated (García-Maroto et al, 1990;Medina et alA993;Mena et al, 1992). …”
Section: Allergenic Propertíesmentioning
confidence: 99%
“…In this study, we focused our attention on a-amylase and trypsin inhibitors in grains, which block the key digestive hydrolases of insect guts, resulting in impaired insect growth. Since immunological and clinical data also indicate that the a-amylase/trypsin inhibitor family can lead to allergies, baker's asthma (a respiratory allergy to wheat proteins) and atopic dermatitis in humans (Mena et al 1992;Sanchezmonge et al 1992;Imaizumi et al 2000;Tatham & Shewry 2008), we also checked their specificity against human salivary and pancreatic amylases.…”
Section: Introductionmentioning
confidence: 99%