A major facilitator superfamily transporter in Colletotrichum fructicola (CfMfs1) is required for sugar transport, appressorial turgor pressure, conidiation and pathogenicity

Abstract

The major facilitator superfamily (MFS) is one of the largest membrane‐protein families. To investigate the role of MFS proteins in the fungal plant anthracnose pathogen Colletotrichum fructicola, the CfMFS1 gene was deleted. This resulted in reduced mycelial growth, conidial yield and decreased virulence on tea oil camellia leaves. In addition, ∆Cfmfs1 showed increased sensitivity to osmotic stress and to a cell‐wall stressor. Further analysis revealed that CfMfs1 is required for conidial penetration and appressorial turgor pressure, both important for fungal pathogen invasion. Confocal fluorescence microscopy showed that CfMfs1 is localized to membranes of both hyphae and conidia, suggesting that it may be a membrane transporter. Our study provides evidence that CfMfs1 has a role in conidiation, sugar transport, stress response, conidial penetration, appressorial turgor pressure and virulence against tea oil camellia.

A HOPS protein, CfVps39, is required for appressorium formation, environmental stress response and vacuolar fusion of Colletotrichum fructicola

“…Anthracnose is one of the main diseases of Ca . oleifera , causing flowers, fruits, and buds to fall and even the whole plant to die, resulting in severe economic losses (Chen et al, 2019).…”

“…fructicola were studied. Previously, we had shown that CfSNF1 , CfSTE50 , CfMFS1 and CfHAC1 were involved in regulating growth, stress response and pathogenicity (Chen et al, 2019, 2020; Yao et al, 2019; Zhang et al, 2019). Shang et al (2020) studied the effector molecule CfEC92 of C .…”

“…In this preliminary study, the functions of some genes of C. fructicola were studied. Previously, we had shown that CfSNF1, CfSTE50, CfMFS1 and CfHAC1 were involved in regulating growth, stress response and pathogenicity (Chen et al, 2019(Chen et al, , 2020Yao et al, 2019;Zhang et al, 2019). Shang et al (2020) studied the effector molecule CfEC92 of C. fructicola and found that the effector molecule inhibits the immune response of plants in the early stage of infection (Shang et al, 2020).…”

CgMFS1, a Major Facilitator Superfamily Transporter, Is Required for Sugar Transport, Oxidative Stress Resistance, and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis

“…The MFS1 gene of Colletotrichum lindemuthianum is only expressed at necrotrophic phase and is required for sugar utilization during interaction within the plant host [15]. In the Colletotrichum fructicola, the fungal pathogen of the tea oil camellia plant, the MFS transporter CfMfs1 has a role in sugar transport; in addition, the transporter also plays roles in conidiation and pathogenicity [16]. Bcmfs1 is involved in the protection of Botrytis cinerea against natural plant defense compounds during the pathogenic phase and antimicrobial compounds during its saprophytic growth phase [17].…”

Genome-Wide Analysis of Major Facilitator Superfamily and Its Expression in Response of Poplar to Fusarium oxysporum

“…The major facilitator superfamily (MFS) is one of the largest membrane transporter families currently known ( Chen et al, 2019 ). MFS’s diverse members are found in essentially all organisms in the biosphere.…”